TY - JOUR
T1 - Human primary macrophages scavenge AuNPs and eliminate it through exosomes. A natural shuttling for nanomaterials
AU - Logozzi, Mariantonia
AU - Mizzoni, Davide
AU - Bocca, Beatrice
AU - Di Raimo, Rossella
AU - Petrucci, Francesco
AU - Caimi, Stefano
AU - Alimonti, Alessandro
AU - Falchi, Mario
AU - Cappello, Francesco
AU - Campanella, Claudia
AU - Bavisotto, Celeste Caruso
AU - David, Sabrina
AU - Bucchieri, Fabio
AU - Angelini, Daniela F.
AU - Battistini, Luca
AU - Fais, Stefano
PY - 2019/4/1
Y1 - 2019/4/1
N2 - The use of nanomaterials is increasing but the real risk associated with their use in humans has to be defined. In fact, nanomaterials tend to accumulate in organs over a long period of time and are slowly degraded or eliminated by the body. Exosomes are nanovesicles actively shuttle molecules, including chemical products and metals, through the body. Macrophages scavenge the body from both organic and inorganic substances, and they use to release high amounts of exosomes. We hypothesized that macrophages may have a role in eliminating nanomaterials through their exosomes. We treated human primary macrophages with 20 nm gold nanoparticles (AuNPs), analyzing the presence of AuNPs in both cells and the released exosomes by the implementation of different techniques, including SP-ICP-MS and NTA. We showed that macrophages endocytosed AuNPs and released them through exosomes. Our study on one hand provide the evidence for a new methodology in the early identification of the nanomaterials levels in exposed subjects. On the other hand we depict a way our body shuttle virtually intact nanoparticles through macrophage-released exosomes.
AB - The use of nanomaterials is increasing but the real risk associated with their use in humans has to be defined. In fact, nanomaterials tend to accumulate in organs over a long period of time and are slowly degraded or eliminated by the body. Exosomes are nanovesicles actively shuttle molecules, including chemical products and metals, through the body. Macrophages scavenge the body from both organic and inorganic substances, and they use to release high amounts of exosomes. We hypothesized that macrophages may have a role in eliminating nanomaterials through their exosomes. We treated human primary macrophages with 20 nm gold nanoparticles (AuNPs), analyzing the presence of AuNPs in both cells and the released exosomes by the implementation of different techniques, including SP-ICP-MS and NTA. We showed that macrophages endocytosed AuNPs and released them through exosomes. Our study on one hand provide the evidence for a new methodology in the early identification of the nanomaterials levels in exposed subjects. On the other hand we depict a way our body shuttle virtually intact nanoparticles through macrophage-released exosomes.
U2 - 10.1016/j.ejpb.2019.02.014
DO - 10.1016/j.ejpb.2019.02.014
M3 - Articolo
VL - 137
SP - 23
EP - 36
JO - European Journal of Pharmaceutics and Biopharmaceutics
JF - European Journal of Pharmaceutics and Biopharmaceutics
SN - 0939-6411
ER -