Human recombinant IGF-I induces the functional expression of AMPA/kainate receptors in cerebellar granule cells

Cristina Zona, Maria Teresa Ciotti, Pietro Calissano

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

We have previously reported that an excitatory amino acid resistant phenotype (EAA-) of rat cerebellar granule cells becomes EAA sensitive (EAA+) when cultured in the presence of human recombinant IGF-I. In order to assess the mechanism through which this somatomedin upmodulates the functional expression EAAs receptors, we have performed studies using the whole-cell configuration of the patch-clamp technique to study macroscopic currents evoked by the application in the bath of kainate to (EAA-) and (EAA+) neurons, and compared their properties to sister cultures grown in classical conditions employing whole faetal calf serum (FCS). Kainate elicited macroscopic, 6-cyano-7-nitroquinoxaline-2,3-dione sensitive, inward currents at a holding potential of -60 mV in almost all the patched cells but the mean amplitude of the current was consistenly smaller in (EAA-) neurons compared to (EAA+) neurons although the amplitude was still smaller than that observed in FCS-cultured neurons. The amplitude of the responses induced by kainate was a linear function of the membrane potential in the three groups of cells and reversal potential of the currents was about 0 mV, suggesting that the general property of each channel is identical in all three types of neurons while th different conductances observed are due either to a decreased expression, a different permeability or an altered affinity of the single receptor.

Original languageEnglish
Pages (from-to)75-78
Number of pages4
JournalNeuroscience Letters
Volume186
Issue number2-3
DOIs
Publication statusPublished - Feb 17 1995

Fingerprint

Kainic Acid Receptors
AMPA Receptors
Insulin-Like Growth Factor I
Kainic Acid
Neurons
6-Cyano-7-nitroquinoxaline-2,3-dione
Excitatory Amino Acids
Patch-Clamp Techniques
Somatomedins
Serum
Baths
Membrane Potentials
Permeability
Phenotype

Keywords

  • Cerebellar granule cells
  • Growth factors
  • Kainate induced currents
  • Patch-clamp

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Human recombinant IGF-I induces the functional expression of AMPA/kainate receptors in cerebellar granule cells. / Zona, Cristina; Ciotti, Maria Teresa; Calissano, Pietro.

In: Neuroscience Letters, Vol. 186, No. 2-3, 17.02.1995, p. 75-78.

Research output: Contribution to journalArticle

@article{a17eb69d6b52454f81fe737f7fb18fd8,
title = "Human recombinant IGF-I induces the functional expression of AMPA/kainate receptors in cerebellar granule cells",
abstract = "We have previously reported that an excitatory amino acid resistant phenotype (EAA-) of rat cerebellar granule cells becomes EAA sensitive (EAA+) when cultured in the presence of human recombinant IGF-I. In order to assess the mechanism through which this somatomedin upmodulates the functional expression EAAs receptors, we have performed studies using the whole-cell configuration of the patch-clamp technique to study macroscopic currents evoked by the application in the bath of kainate to (EAA-) and (EAA+) neurons, and compared their properties to sister cultures grown in classical conditions employing whole faetal calf serum (FCS). Kainate elicited macroscopic, 6-cyano-7-nitroquinoxaline-2,3-dione sensitive, inward currents at a holding potential of -60 mV in almost all the patched cells but the mean amplitude of the current was consistenly smaller in (EAA-) neurons compared to (EAA+) neurons although the amplitude was still smaller than that observed in FCS-cultured neurons. The amplitude of the responses induced by kainate was a linear function of the membrane potential in the three groups of cells and reversal potential of the currents was about 0 mV, suggesting that the general property of each channel is identical in all three types of neurons while th different conductances observed are due either to a decreased expression, a different permeability or an altered affinity of the single receptor.",
keywords = "Cerebellar granule cells, Growth factors, Kainate induced currents, Patch-clamp",
author = "Cristina Zona and Ciotti, {Maria Teresa} and Pietro Calissano",
year = "1995",
month = "2",
day = "17",
doi = "10.1016/0304-3940(95)11284-4",
language = "English",
volume = "186",
pages = "75--78",
journal = "Neuroscience Letters",
issn = "0304-3940",
publisher = "Elsevier Ireland Ltd",
number = "2-3",

}

TY - JOUR

T1 - Human recombinant IGF-I induces the functional expression of AMPA/kainate receptors in cerebellar granule cells

AU - Zona, Cristina

AU - Ciotti, Maria Teresa

AU - Calissano, Pietro

PY - 1995/2/17

Y1 - 1995/2/17

N2 - We have previously reported that an excitatory amino acid resistant phenotype (EAA-) of rat cerebellar granule cells becomes EAA sensitive (EAA+) when cultured in the presence of human recombinant IGF-I. In order to assess the mechanism through which this somatomedin upmodulates the functional expression EAAs receptors, we have performed studies using the whole-cell configuration of the patch-clamp technique to study macroscopic currents evoked by the application in the bath of kainate to (EAA-) and (EAA+) neurons, and compared their properties to sister cultures grown in classical conditions employing whole faetal calf serum (FCS). Kainate elicited macroscopic, 6-cyano-7-nitroquinoxaline-2,3-dione sensitive, inward currents at a holding potential of -60 mV in almost all the patched cells but the mean amplitude of the current was consistenly smaller in (EAA-) neurons compared to (EAA+) neurons although the amplitude was still smaller than that observed in FCS-cultured neurons. The amplitude of the responses induced by kainate was a linear function of the membrane potential in the three groups of cells and reversal potential of the currents was about 0 mV, suggesting that the general property of each channel is identical in all three types of neurons while th different conductances observed are due either to a decreased expression, a different permeability or an altered affinity of the single receptor.

AB - We have previously reported that an excitatory amino acid resistant phenotype (EAA-) of rat cerebellar granule cells becomes EAA sensitive (EAA+) when cultured in the presence of human recombinant IGF-I. In order to assess the mechanism through which this somatomedin upmodulates the functional expression EAAs receptors, we have performed studies using the whole-cell configuration of the patch-clamp technique to study macroscopic currents evoked by the application in the bath of kainate to (EAA-) and (EAA+) neurons, and compared their properties to sister cultures grown in classical conditions employing whole faetal calf serum (FCS). Kainate elicited macroscopic, 6-cyano-7-nitroquinoxaline-2,3-dione sensitive, inward currents at a holding potential of -60 mV in almost all the patched cells but the mean amplitude of the current was consistenly smaller in (EAA-) neurons compared to (EAA+) neurons although the amplitude was still smaller than that observed in FCS-cultured neurons. The amplitude of the responses induced by kainate was a linear function of the membrane potential in the three groups of cells and reversal potential of the currents was about 0 mV, suggesting that the general property of each channel is identical in all three types of neurons while th different conductances observed are due either to a decreased expression, a different permeability or an altered affinity of the single receptor.

KW - Cerebellar granule cells

KW - Growth factors

KW - Kainate induced currents

KW - Patch-clamp

UR - http://www.scopus.com/inward/record.url?scp=0028944979&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028944979&partnerID=8YFLogxK

U2 - 10.1016/0304-3940(95)11284-4

DO - 10.1016/0304-3940(95)11284-4

M3 - Article

C2 - 7777202

AN - SCOPUS:0028944979

VL - 186

SP - 75

EP - 78

JO - Neuroscience Letters

JF - Neuroscience Letters

SN - 0304-3940

IS - 2-3

ER -