Human recombinant IGF-I induces the functional expression of AMPA/kainate receptors in cerebellar granule cells

Cristina Zona, Maria Teresa Ciotti, Pietro Calissano

Research output: Contribution to journalArticle


We have previously reported that an excitatory amino acid resistant phenotype (EAA-) of rat cerebellar granule cells becomes EAA sensitive (EAA+) when cultured in the presence of human recombinant IGF-I. In order to assess the mechanism through which this somatomedin upmodulates the functional expression EAAs receptors, we have performed studies using the whole-cell configuration of the patch-clamp technique to study macroscopic currents evoked by the application in the bath of kainate to (EAA-) and (EAA+) neurons, and compared their properties to sister cultures grown in classical conditions employing whole faetal calf serum (FCS). Kainate elicited macroscopic, 6-cyano-7-nitroquinoxaline-2,3-dione sensitive, inward currents at a holding potential of -60 mV in almost all the patched cells but the mean amplitude of the current was consistenly smaller in (EAA-) neurons compared to (EAA+) neurons although the amplitude was still smaller than that observed in FCS-cultured neurons. The amplitude of the responses induced by kainate was a linear function of the membrane potential in the three groups of cells and reversal potential of the currents was about 0 mV, suggesting that the general property of each channel is identical in all three types of neurons while th different conductances observed are due either to a decreased expression, a different permeability or an altered affinity of the single receptor.

Original languageEnglish
Pages (from-to)75-78
Number of pages4
JournalNeuroscience Letters
Issue number2-3
Publication statusPublished - Feb 17 1995


  • Cerebellar granule cells
  • Growth factors
  • Kainate induced currents
  • Patch-clamp

ASJC Scopus subject areas

  • Neuroscience(all)

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