Human renal epithelial cells produce the long pentraxin PTX3

Alma J. Nauta, Simone De Haij, Barbara Bottazzi, Alberto Mantovani, Maria C. Borrias, Jan Aten, Maria Pia Rastaldi, Mohamed R. Daha, Cees Van Kooten, Anja Roos

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

Background. Pentraxin 3 (PTX3) is a prototypic long pentraxin with structural similarities in the C-terminal domain to the classical short pentraxins C-reactive protein (CRP) and serum amyloid P component. PTX3 is suggested to play an important role in the innate resistance against pathogens, regulation of inflammatory reactions, and clearance of apoptotic cells. Unlike the classic pentraxins, PTX3 is mainly expressed extra-hepatically. The present study was designed to investigate the expression of PTX3 by human proximal renal tubular epithelial cells (PTECs). Methods. PTECs were cultured in the presence or absence of inflammatory cytokines. PTX3 mRNA expression was measured by reverse transcription-polymerase chain reaction (RT-PCR) in human kidney and PTECs. PTX3 protein levels in PTEC cultures were quantified by enzyme-linked immunosorbent assay (ELISA). Results. PTX3 mRNA was shown to be constitutively expressed in human kidney. Constitutive expression and production of PTX3 was shown in primary mesangial cells, in primary PTECs, and in renal fibroblasts. Further analysis showed that interleukin (IL)-1 and tumor necrosis factor-α (TNF-α) stimulation strongly enhanced the expression and production of PTX3 in PTECs in a dose- and time-dependent manner. In addition, activation of PTECs with IL-17 and CD40L, respectively, but not with IL-6 or IL-4, resulted in strongly increased production of PTX3, whereas granulocyte macrophage-colony-stimulating factor (GM-CSF) inhibited IL-1-induced PTX3 production. PTX3 produced by PTEC is functionally active in binding C1q. Conclusion. These results indicate that PTX3 is expressed and released by PTECs and that in proinflammatory conditions PTX3 production is up-regulated. Local expression of PTX3 may play a role in the innate immune response and inflammatory reactions in the kidney.

Original languageEnglish
Pages (from-to)543-553
Number of pages11
JournalKidney International
Volume67
Issue number2
DOIs
Publication statusPublished - Feb 2005

Fingerprint

Epithelial Cells
Kidney
PTX3 protein
Interleukin-1
Serum Amyloid P-Component
Messenger RNA
CD40 Ligand
Mesangial Cells
Interleukin-17
Granulocyte-Macrophage Colony-Stimulating Factor
Innate Immunity
Interleukin-4
C-Reactive Protein
Reverse Transcription
Interleukin-6
Cell Culture Techniques
Tumor Necrosis Factor-alpha
Fibroblasts
Enzyme-Linked Immunosorbent Assay
Cytokines

Keywords

  • Inflammation
  • Pentraxin 3
  • Tubular epithelial cells

ASJC Scopus subject areas

  • Nephrology

Cite this

Human renal epithelial cells produce the long pentraxin PTX3. / Nauta, Alma J.; De Haij, Simone; Bottazzi, Barbara; Mantovani, Alberto; Borrias, Maria C.; Aten, Jan; Rastaldi, Maria Pia; Daha, Mohamed R.; Van Kooten, Cees; Roos, Anja.

In: Kidney International, Vol. 67, No. 2, 02.2005, p. 543-553.

Research output: Contribution to journalArticle

Nauta, AJ, De Haij, S, Bottazzi, B, Mantovani, A, Borrias, MC, Aten, J, Rastaldi, MP, Daha, MR, Van Kooten, C & Roos, A 2005, 'Human renal epithelial cells produce the long pentraxin PTX3', Kidney International, vol. 67, no. 2, pp. 543-553. https://doi.org/10.1111/j.1523-1755.2005.67111.x
Nauta, Alma J. ; De Haij, Simone ; Bottazzi, Barbara ; Mantovani, Alberto ; Borrias, Maria C. ; Aten, Jan ; Rastaldi, Maria Pia ; Daha, Mohamed R. ; Van Kooten, Cees ; Roos, Anja. / Human renal epithelial cells produce the long pentraxin PTX3. In: Kidney International. 2005 ; Vol. 67, No. 2. pp. 543-553.
@article{40e5cf6c27dd4a3280bb1e548e9dea81,
title = "Human renal epithelial cells produce the long pentraxin PTX3",
abstract = "Background. Pentraxin 3 (PTX3) is a prototypic long pentraxin with structural similarities in the C-terminal domain to the classical short pentraxins C-reactive protein (CRP) and serum amyloid P component. PTX3 is suggested to play an important role in the innate resistance against pathogens, regulation of inflammatory reactions, and clearance of apoptotic cells. Unlike the classic pentraxins, PTX3 is mainly expressed extra-hepatically. The present study was designed to investigate the expression of PTX3 by human proximal renal tubular epithelial cells (PTECs). Methods. PTECs were cultured in the presence or absence of inflammatory cytokines. PTX3 mRNA expression was measured by reverse transcription-polymerase chain reaction (RT-PCR) in human kidney and PTECs. PTX3 protein levels in PTEC cultures were quantified by enzyme-linked immunosorbent assay (ELISA). Results. PTX3 mRNA was shown to be constitutively expressed in human kidney. Constitutive expression and production of PTX3 was shown in primary mesangial cells, in primary PTECs, and in renal fibroblasts. Further analysis showed that interleukin (IL)-1 and tumor necrosis factor-α (TNF-α) stimulation strongly enhanced the expression and production of PTX3 in PTECs in a dose- and time-dependent manner. In addition, activation of PTECs with IL-17 and CD40L, respectively, but not with IL-6 or IL-4, resulted in strongly increased production of PTX3, whereas granulocyte macrophage-colony-stimulating factor (GM-CSF) inhibited IL-1-induced PTX3 production. PTX3 produced by PTEC is functionally active in binding C1q. Conclusion. These results indicate that PTX3 is expressed and released by PTECs and that in proinflammatory conditions PTX3 production is up-regulated. Local expression of PTX3 may play a role in the innate immune response and inflammatory reactions in the kidney.",
keywords = "Inflammation, Pentraxin 3, Tubular epithelial cells",
author = "Nauta, {Alma J.} and {De Haij}, Simone and Barbara Bottazzi and Alberto Mantovani and Borrias, {Maria C.} and Jan Aten and Rastaldi, {Maria Pia} and Daha, {Mohamed R.} and {Van Kooten}, Cees and Anja Roos",
year = "2005",
month = "2",
doi = "10.1111/j.1523-1755.2005.67111.x",
language = "English",
volume = "67",
pages = "543--553",
journal = "Kidney International",
issn = "0085-2538",
publisher = "Nature Publishing Group",
number = "2",

}

TY - JOUR

T1 - Human renal epithelial cells produce the long pentraxin PTX3

AU - Nauta, Alma J.

AU - De Haij, Simone

AU - Bottazzi, Barbara

AU - Mantovani, Alberto

AU - Borrias, Maria C.

AU - Aten, Jan

AU - Rastaldi, Maria Pia

AU - Daha, Mohamed R.

AU - Van Kooten, Cees

AU - Roos, Anja

PY - 2005/2

Y1 - 2005/2

N2 - Background. Pentraxin 3 (PTX3) is a prototypic long pentraxin with structural similarities in the C-terminal domain to the classical short pentraxins C-reactive protein (CRP) and serum amyloid P component. PTX3 is suggested to play an important role in the innate resistance against pathogens, regulation of inflammatory reactions, and clearance of apoptotic cells. Unlike the classic pentraxins, PTX3 is mainly expressed extra-hepatically. The present study was designed to investigate the expression of PTX3 by human proximal renal tubular epithelial cells (PTECs). Methods. PTECs were cultured in the presence or absence of inflammatory cytokines. PTX3 mRNA expression was measured by reverse transcription-polymerase chain reaction (RT-PCR) in human kidney and PTECs. PTX3 protein levels in PTEC cultures were quantified by enzyme-linked immunosorbent assay (ELISA). Results. PTX3 mRNA was shown to be constitutively expressed in human kidney. Constitutive expression and production of PTX3 was shown in primary mesangial cells, in primary PTECs, and in renal fibroblasts. Further analysis showed that interleukin (IL)-1 and tumor necrosis factor-α (TNF-α) stimulation strongly enhanced the expression and production of PTX3 in PTECs in a dose- and time-dependent manner. In addition, activation of PTECs with IL-17 and CD40L, respectively, but not with IL-6 or IL-4, resulted in strongly increased production of PTX3, whereas granulocyte macrophage-colony-stimulating factor (GM-CSF) inhibited IL-1-induced PTX3 production. PTX3 produced by PTEC is functionally active in binding C1q. Conclusion. These results indicate that PTX3 is expressed and released by PTECs and that in proinflammatory conditions PTX3 production is up-regulated. Local expression of PTX3 may play a role in the innate immune response and inflammatory reactions in the kidney.

AB - Background. Pentraxin 3 (PTX3) is a prototypic long pentraxin with structural similarities in the C-terminal domain to the classical short pentraxins C-reactive protein (CRP) and serum amyloid P component. PTX3 is suggested to play an important role in the innate resistance against pathogens, regulation of inflammatory reactions, and clearance of apoptotic cells. Unlike the classic pentraxins, PTX3 is mainly expressed extra-hepatically. The present study was designed to investigate the expression of PTX3 by human proximal renal tubular epithelial cells (PTECs). Methods. PTECs were cultured in the presence or absence of inflammatory cytokines. PTX3 mRNA expression was measured by reverse transcription-polymerase chain reaction (RT-PCR) in human kidney and PTECs. PTX3 protein levels in PTEC cultures were quantified by enzyme-linked immunosorbent assay (ELISA). Results. PTX3 mRNA was shown to be constitutively expressed in human kidney. Constitutive expression and production of PTX3 was shown in primary mesangial cells, in primary PTECs, and in renal fibroblasts. Further analysis showed that interleukin (IL)-1 and tumor necrosis factor-α (TNF-α) stimulation strongly enhanced the expression and production of PTX3 in PTECs in a dose- and time-dependent manner. In addition, activation of PTECs with IL-17 and CD40L, respectively, but not with IL-6 or IL-4, resulted in strongly increased production of PTX3, whereas granulocyte macrophage-colony-stimulating factor (GM-CSF) inhibited IL-1-induced PTX3 production. PTX3 produced by PTEC is functionally active in binding C1q. Conclusion. These results indicate that PTX3 is expressed and released by PTECs and that in proinflammatory conditions PTX3 production is up-regulated. Local expression of PTX3 may play a role in the innate immune response and inflammatory reactions in the kidney.

KW - Inflammation

KW - Pentraxin 3

KW - Tubular epithelial cells

UR - http://www.scopus.com/inward/record.url?scp=19944434100&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=19944434100&partnerID=8YFLogxK

U2 - 10.1111/j.1523-1755.2005.67111.x

DO - 10.1111/j.1523-1755.2005.67111.x

M3 - Article

C2 - 15673302

AN - SCOPUS:19944434100

VL - 67

SP - 543

EP - 553

JO - Kidney International

JF - Kidney International

SN - 0085-2538

IS - 2

ER -