TY - JOUR
T1 - Human respiratory coronavirus HKU1 versus other coronavirus infections in Italian hospitalised patients
AU - Gerna, Giuseppe
AU - Percivalle, Elena
AU - Sarasini, Antonella
AU - Campanini, Giulia
AU - Piralla, Antonio
AU - Rovida, Francesca
AU - Genini, Emilia
AU - Marchi, Antonietta
AU - Baldanti, Fausto
PY - 2007/3
Y1 - 2007/3
N2 - Background: Human respiratory coronavirus (hCoV) HKU1 infections were reported for the first time in 2005 in Hong Kong. Objective: To investigate epidemiological, clinical, and diagnostic features of HKU1 infections. Study design: Longitudinal, prospective study from November 2005 through May 2006 in a hospitalised patient population. Results: Overall, 48/426 (11.3%) patients were found to be infected by hCoV acute respiratory tract infections (ARTI). Of these, 10 (19.2%) were caused by HKU1 (6 single infections and 4 coinfections) during the period January-May 2006. Diagnosis was made by using RT-PCR for all four hCoVs, and in parallel, in-house developed group-specific monoclonal antibodies (MAbs) for HKU1 and 229E. HKU1-specific MAb was able to retrospectively identify 8 of 10 HKU1 strains detected by RT-PCR. Phylogenetic analysis showed that four HKU1 strains were genotype A and six genotype B. In HKU1-infected patients, the predominant clinical symptom was rhinorrhea (nine patients). Within group II hCoV, HKU1-infected patients had a significantly lower rate of lower ARTI compared to OC43-infected patients. Conclusion: HKU1 hCoV strains circulated in northern Italy during the winter-spring season 2005-2006. Both HKU1 genotypes were detected. HKU1-specific MAb may contribute to the rapid diagnosis of HKU1 infections currently performed by RT-PCR.
AB - Background: Human respiratory coronavirus (hCoV) HKU1 infections were reported for the first time in 2005 in Hong Kong. Objective: To investigate epidemiological, clinical, and diagnostic features of HKU1 infections. Study design: Longitudinal, prospective study from November 2005 through May 2006 in a hospitalised patient population. Results: Overall, 48/426 (11.3%) patients were found to be infected by hCoV acute respiratory tract infections (ARTI). Of these, 10 (19.2%) were caused by HKU1 (6 single infections and 4 coinfections) during the period January-May 2006. Diagnosis was made by using RT-PCR for all four hCoVs, and in parallel, in-house developed group-specific monoclonal antibodies (MAbs) for HKU1 and 229E. HKU1-specific MAb was able to retrospectively identify 8 of 10 HKU1 strains detected by RT-PCR. Phylogenetic analysis showed that four HKU1 strains were genotype A and six genotype B. In HKU1-infected patients, the predominant clinical symptom was rhinorrhea (nine patients). Within group II hCoV, HKU1-infected patients had a significantly lower rate of lower ARTI compared to OC43-infected patients. Conclusion: HKU1 hCoV strains circulated in northern Italy during the winter-spring season 2005-2006. Both HKU1 genotypes were detected. HKU1-specific MAb may contribute to the rapid diagnosis of HKU1 infections currently performed by RT-PCR.
KW - Acute respiratory tract infections
KW - HKU1 genotypes
KW - Human coronavirus HKU1
KW - Monoclonal antibody
KW - Reverse transcription-PCR
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U2 - 10.1016/j.jcv.2006.12.008
DO - 10.1016/j.jcv.2006.12.008
M3 - Article
C2 - 17222582
AN - SCOPUS:33847053190
VL - 38
SP - 244
EP - 250
JO - Journal of Clinical Virology
JF - Journal of Clinical Virology
SN - 1386-6532
IS - 3
ER -