Human serum albumin Cys34 oxidative modifications following infiltration in the carotid atherosclerotic plaque

Antonio Junior Lepedda, Angelo Zinellu, Gabriele Nieddu, Pierina De Muro, Ciriaco Carru, Rita Spirito, Anna Guarino, Franco Piredda, Marilena Formato

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Abstract

Objectives. To evaluate if the prooxidant environment present in atherosclerotic plaque may oxidatively modify filtered albumin. Methods. Fluorescein-5-maleimide labelled plasma samples and plaque extracts from 27 patients who had undergone carotid endarterectomy were analysed through nonreducing SDS-PAGE for albumin-Cys34 oxidation. Furthermore, degree and pattern of S-thiolation in both circulating and plaque-filtered albumin were assayed. Results. Albumin filtered in the atherosclerotic plaque showed higher levels of Cys34 oxidative modifications than the corresponding circulating form as well as different patterns of S-thiolation. Conclusions. Data indicate that the circulating albumin, once filtered in plaque, undergoes Cys34 oxidative modifications and demonstrate for the first time that albumin is a homocysteine and cysteinylglycine vehicle inside the plaque environment.

Original languageEnglish
Article number690953
JournalOxidative Medicine and Cellular Longevity
Volume2014
DOIs
Publication statusPublished - 2014

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ASJC Scopus subject areas

  • Cell Biology
  • Ageing
  • Biochemistry
  • Medicine(all)

Cite this

Lepedda, A. J., Zinellu, A., Nieddu, G., De Muro, P., Carru, C., Spirito, R., Guarino, A., Piredda, F., & Formato, M. (2014). Human serum albumin Cys34 oxidative modifications following infiltration in the carotid atherosclerotic plaque. Oxidative Medicine and Cellular Longevity, 2014, [690953]. https://doi.org/10.1155/2014/690953