Human serum antibodies to a major defined epitope of human herpesvirus 8 small viral capsid antigen

Rosamaria Tedeschi, Paolo De Paoli, Thomas F. Schulz, Joakim Dillner

Research output: Contribution to journalArticle

Abstract

The major antibody-reactive epitope of the small viral capsid antigen (sVCA) of human herpesvirus 8 (HHV-8) was defined by use of overlapping peptides. Strong IgG reactivity was found among ~50% of 44 human immunodeficiency virus-positive or -negative patients with Kaposi's sarcoma and 13 subjects who were seropositive by immunofluorescence assay (IFA) for the latent HHV-8 nuclear antigen. Only 1 of 106 subjects seronegative for both lytic and latent HHV-8 antigens and 10 of 81 subjects IFA-seropositive only for the lytic HHV-8 antigen had strong IgG reactivity to this epitope. Among 534 healthy Swedish women, only 1.3% were strongly seropositive. Comparison of the peptide-based and purified sVCA protein-based ELISAs found 55% sensitivity and 98% specificity. However, only 1 of 452 serum samples from healthy women was positive in both tests. In conclusion, the defined sVCA epitope was a specific, but not very sensitive, serologic marker of active HHV-8 infection. Such infection appears to be rare among Swedish women, even with sexual risk-taking behavior.

Original languageEnglish
Pages (from-to)1016-1020
Number of pages5
JournalJournal of Infectious Diseases
Volume179
Issue number4
DOIs
Publication statusPublished - 1999

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

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