Human serum-dependent survival of GM-CFCs in vitro from patients with chronic granulocytic leukemia

Francesco Frassoni, Mario Repetto, Giovanna Piaggio, Marina Podesta, Alberto M. Marmont

Research output: Contribution to journalArticlepeer-review

Abstract

Bone marrow (BM) cells from 15 patients with chronic granulocytic leukemia (CGL) and 12 normal donors were placed in liquid culture for 4-7 days in the presence of fetal calf serum (FCS) or human AB serum. BM cells were plated in agar for GM-CFC growth at day 0, day 4, day 7 of culture, in the presence of human placenta conditioned medium (HPCM). The recovery of GM-CFCs on day 4 in FCS was 86 ± 18 and 15 ± 18% from normal or CGL BM cells respectively (p = 0.005). The recovery of GM-CFCs on day 4 in human AB serum was comparable for normal (66 ± 48%) and CGL (69 ± 32%) BM cells. Similar results were obtained on day 7 of culture. Cytochemical staining of agar plates showed a sharp drop of macrophage colonies in CGL BM cells kept in FCS cultures on day 4-7, when compared to both normal BM cells and baseline colonies. These data suggest that the survival in liquid culture of GM-CFCs from patients with CGL is dependent on a factor present in human and not in fetal calf serum. This is not the case for normal GM-CFCs.

Original languageEnglish
Pages (from-to)3-6
Number of pages4
JournalLeukemia Research
Volume11
Issue number1
DOIs
Publication statusPublished - 1987

Keywords

  • CSFs
  • GM-CFC
  • growth factors
  • leukemia
  • survival

ASJC Scopus subject areas

  • Cancer Research
  • Hematology
  • Oncology

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