TY - JOUR
T1 - Human serum ferritin G-peptide is recognized by anti-L ferritin subunit antibodies and concanavalin-A
AU - Santambrogio, P.
AU - Cozzi, A.
AU - Levi, S.
AU - Arosio, P.
PY - 1987
Y1 - 1987
N2 - Ferritin was purified from serum of patients with idiopathic haemochromatosis. Analysis on SDS electrophoresis showed that it is composed of two major bands of 19,000 and 23,000 Mr. The smaller peptide has an electrophoretic mobility and immunochemical reactivity similar to that of tissue L subunit. The larger, previously named G subunit, is recognized by concanavalin-A and by anti-ferritin L-subunit, but not by anti-H, monoclonal antibodies. All of the antibodies show higher affinity for the L than for the G subunit. Therefore, the G chain appears immunochemically similar, but not identical, to ferritin L chain, and is responsible for serum ferritin binding concanavalin-A.
AB - Ferritin was purified from serum of patients with idiopathic haemochromatosis. Analysis on SDS electrophoresis showed that it is composed of two major bands of 19,000 and 23,000 Mr. The smaller peptide has an electrophoretic mobility and immunochemical reactivity similar to that of tissue L subunit. The larger, previously named G subunit, is recognized by concanavalin-A and by anti-ferritin L-subunit, but not by anti-H, monoclonal antibodies. All of the antibodies show higher affinity for the L than for the G subunit. Therefore, the G chain appears immunochemically similar, but not identical, to ferritin L chain, and is responsible for serum ferritin binding concanavalin-A.
UR - http://www.scopus.com/inward/record.url?scp=0023155810&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023155810&partnerID=8YFLogxK
M3 - Article
C2 - 3828232
AN - SCOPUS:0023155810
VL - 65
SP - 235
EP - 237
JO - British Journal of Haematology
JF - British Journal of Haematology
SN - 0007-1048
IS - 2
ER -