Human skin fibroblast culture to test glycolic and lactic acid solutions

Alphahydroxy acids (AHAs) have become quite popular as skin rejuvenating agents. The action of AHAs on the skin is affected by the contact time, the concentration and the pH of the solution used. In a previous in vitro study we showed that by using salified derivatives of glycolic and lactic acid one may prolong the contact time for longer than with the pure form. In the present work we test the effects of different concentration of natural glycolic and lactic acid at a pH between 4.5 and 5.5, combined with a special protective mixture formed of gelatine-glycine-aminoacids at the same pH. The investigations performed brought to light no significant differences among the various solution employed. Further study are necessary in order to obtain in vitro results matching the in vivo clinical experience.