TY - JOUR
T1 - Human spermatozoa cryopreservation
T2 - Comparison of three different freezing protocols
AU - Omes, Claudia
AU - Marchetti, Anna Lisa
AU - Masanti, Maria Luisa
AU - Bassani, Roberto
AU - Tinelli, Carmine
AU - Sanarica, Rossella Chiara
AU - Spinillo, Arsenio
AU - Nappi, Rossella Elena
PY - 2013/9
Y1 - 2013/9
N2 - Human spermatozoa cryopreservation has significantly improved over the last few decades, but the actual protocols are neither optimal nor standardized between different laboratories in spite of the importance of preserving male fertility or treating severely infertile males. In the present study we aimed to determine the best in-house method of rapid freezing in terms of sperm motility and vitality by comparing three different rapid methods of human spermatozoa cryopreservation. Our data showed that Ml (triphasic cooling) is the method associated with a significantly lower deterioration of semen quality in comparison with mono or biphasic cooling. Differences observed among the three protocols were supported by statistical analysis. These data reinforce previous evidences about the influence of sperm quality on IVF outcome and suggest the importance of improving sperm cryopreservation techniques especially when semen is seriously compromised at baseline.
AB - Human spermatozoa cryopreservation has significantly improved over the last few decades, but the actual protocols are neither optimal nor standardized between different laboratories in spite of the importance of preserving male fertility or treating severely infertile males. In the present study we aimed to determine the best in-house method of rapid freezing in terms of sperm motility and vitality by comparing three different rapid methods of human spermatozoa cryopreservation. Our data showed that Ml (triphasic cooling) is the method associated with a significantly lower deterioration of semen quality in comparison with mono or biphasic cooling. Differences observed among the three protocols were supported by statistical analysis. These data reinforce previous evidences about the influence of sperm quality on IVF outcome and suggest the importance of improving sperm cryopreservation techniques especially when semen is seriously compromised at baseline.
KW - Fertility preservation
KW - Freezing protocol
KW - Human spermatozoa cryopreservation
KW - Sperm motility
KW - Sperm viability
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M3 - Article
AN - SCOPUS:84887926089
VL - 34
SP - 535
EP - 543
JO - Cryo-Letters
JF - Cryo-Letters
SN - 0143-2044
IS - 5
ER -