Abstract
This chapter discusses techniques for the production and maintenance of human T cell clones, their growth characteristics and longevity in vitro, culture age-associated changes of parameters such as telomere lengths, DNA damage, and so on. Cloning peripheral T cells from young healthy donors can be performed with a high cloning efficiency (CE), suggesting that these particular cloning conditions are favorable for outgrowth of a majority of T cells. The CE was equally high when the cells were derived from a patient with chronic myelogenous leukemia, a donor putatively subjected to a high level of antigenic stress. CE was also similar in the extra-thymic T cell differentiation cultures employed. According to the protocol for cloning, for ease of handling and as a precaution against contamination, stack plates and aluminum foil should be used. Incubate for about a week and then examine the plates using an inverted microscope. T cell functions are mediated by intracellular signaling via surface receptors such as the antigen receptor and the costimulator CD28 from the membrane through the cytoplasm to the nucleus.
| Original language | English |
|---|---|
| Title of host publication | Handbook of Models for Human Aging |
| Publisher | Elsevier Inc. |
| Pages | 781-792 |
| Number of pages | 12 |
| ISBN (Print) | 9780123693914 |
| DOIs | |
| Publication status | Published - 2006 |
Fingerprint
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
Human T Cell Clones in Long-Term Culture as Models for the Impact of Chronic Antigenic Stress in Aging. / Pawelec, Graham; Tolson, Jon; Mariani, Erminia; Neri, Simona; Solana, Rafael; Dela Rosa, Olga; Forsey, Rosalyn; Larbi, Anis; Barnett, Yvonne; Fülöp, Tamas.
Handbook of Models for Human Aging. Elsevier Inc., 2006. p. 781-792.Research output: Chapter in Book/Report/Conference proceeding › Chapter
}
TY - CHAP
T1 - Human T Cell Clones in Long-Term Culture as Models for the Impact of Chronic Antigenic Stress in Aging
AU - Pawelec, Graham
AU - Tolson, Jon
AU - Mariani, Erminia
AU - Neri, Simona
AU - Solana, Rafael
AU - Dela Rosa, Olga
AU - Forsey, Rosalyn
AU - Larbi, Anis
AU - Barnett, Yvonne
AU - Fülöp, Tamas
PY - 2006
Y1 - 2006
N2 - This chapter discusses techniques for the production and maintenance of human T cell clones, their growth characteristics and longevity in vitro, culture age-associated changes of parameters such as telomere lengths, DNA damage, and so on. Cloning peripheral T cells from young healthy donors can be performed with a high cloning efficiency (CE), suggesting that these particular cloning conditions are favorable for outgrowth of a majority of T cells. The CE was equally high when the cells were derived from a patient with chronic myelogenous leukemia, a donor putatively subjected to a high level of antigenic stress. CE was also similar in the extra-thymic T cell differentiation cultures employed. According to the protocol for cloning, for ease of handling and as a precaution against contamination, stack plates and aluminum foil should be used. Incubate for about a week and then examine the plates using an inverted microscope. T cell functions are mediated by intracellular signaling via surface receptors such as the antigen receptor and the costimulator CD28 from the membrane through the cytoplasm to the nucleus.
AB - This chapter discusses techniques for the production and maintenance of human T cell clones, their growth characteristics and longevity in vitro, culture age-associated changes of parameters such as telomere lengths, DNA damage, and so on. Cloning peripheral T cells from young healthy donors can be performed with a high cloning efficiency (CE), suggesting that these particular cloning conditions are favorable for outgrowth of a majority of T cells. The CE was equally high when the cells were derived from a patient with chronic myelogenous leukemia, a donor putatively subjected to a high level of antigenic stress. CE was also similar in the extra-thymic T cell differentiation cultures employed. According to the protocol for cloning, for ease of handling and as a precaution against contamination, stack plates and aluminum foil should be used. Incubate for about a week and then examine the plates using an inverted microscope. T cell functions are mediated by intracellular signaling via surface receptors such as the antigen receptor and the costimulator CD28 from the membrane through the cytoplasm to the nucleus.
UR - http://www.scopus.com/inward/record.url?scp=33947416281&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33947416281&partnerID=8YFLogxK
U2 - 10.1016/B978-012369391-4/50067-9
DO - 10.1016/B978-012369391-4/50067-9
M3 - Chapter
AN - SCOPUS:33947416281
SN - 9780123693914
SP - 781
EP - 792
BT - Handbook of Models for Human Aging
PB - Elsevier Inc.
ER -