Human T-cell lymphotropic virus tax and Epstein-Barr virus DNA in peripheral blood of multiple sclerosis patients during acute attack

P. Ferrante, E. Omodeo-Zorini, M. R. Zuffolato, R. Mancuso, R. Caldarelli-Stefano, S. Puricelli, M. Mediati, L. Losciale, D. Caputo

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Objectives - A study was performed to determine whether persistent or latent viruses are reactivated during the acute attack in relapsing remitting multiple sclerosis (MS). Material and Methods - DNA of herpes simplex virus type 1 and 2 (HSV-1 and -2), human cytomegalovirus (HCMV), Epstein-Barr virus (EBV), JC virus (JCV) and HTLV-I was searched, using nested polymerase chain reaction (PCR), in peripheral blood mononuclear cells (PBMCs) collected from 14 MS patients on the first day and, twice a week, during an acute attack of the disease. Results - Viral DNA was detected, in at least one PBMC sample, in all the patients. Interestingly, EBV DNA was found in 42.8% of the patients on the first day, while a sharp increase of the HTLV tax-rex DNA frequency (35.7%) was observed on the tenth day. Conclusions - In MS relapse EBV DNA detection is an early, frequent event, while the finding of tax-rex, but not of other HTLV-I genomic regions, is a secondary phenomenon, suggesting that these two factors could interact in the pathogenesis of MS relapses.

Original languageEnglish
Pages (from-to)79-85
Number of pages7
JournalActa Neurologica Scandinavica, Supplement
Volume95
Issue number169
Publication statusPublished - 1997

Fingerprint

Human Herpesvirus 4
Multiple Sclerosis
Viruses
T-Lymphocytes
Human T-lymphotropic virus 1
Human Herpesvirus 2
DNA
Human Herpesvirus 1
Blood Cells
JC Virus
Recurrence
Relapsing-Remitting Multiple Sclerosis
Viral DNA
Acute Disease
Cytomegalovirus
Polymerase Chain Reaction

Keywords

  • acute attack
  • Epstein-Barr virus
  • HTLV tax-rex, HSV-1, HSV-2, human cytomegalovirus, JC virus
  • multiple sclerosis
  • PCR

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)
  • Neurology

Cite this

Ferrante, P., Omodeo-Zorini, E., Zuffolato, M. R., Mancuso, R., Caldarelli-Stefano, R., Puricelli, S., ... Caputo, D. (1997). Human T-cell lymphotropic virus tax and Epstein-Barr virus DNA in peripheral blood of multiple sclerosis patients during acute attack. Acta Neurologica Scandinavica, Supplement, 95(169), 79-85.

Human T-cell lymphotropic virus tax and Epstein-Barr virus DNA in peripheral blood of multiple sclerosis patients during acute attack. / Ferrante, P.; Omodeo-Zorini, E.; Zuffolato, M. R.; Mancuso, R.; Caldarelli-Stefano, R.; Puricelli, S.; Mediati, M.; Losciale, L.; Caputo, D.

In: Acta Neurologica Scandinavica, Supplement, Vol. 95, No. 169, 1997, p. 79-85.

Research output: Contribution to journalArticle

Ferrante, P, Omodeo-Zorini, E, Zuffolato, MR, Mancuso, R, Caldarelli-Stefano, R, Puricelli, S, Mediati, M, Losciale, L & Caputo, D 1997, 'Human T-cell lymphotropic virus tax and Epstein-Barr virus DNA in peripheral blood of multiple sclerosis patients during acute attack', Acta Neurologica Scandinavica, Supplement, vol. 95, no. 169, pp. 79-85.
Ferrante, P. ; Omodeo-Zorini, E. ; Zuffolato, M. R. ; Mancuso, R. ; Caldarelli-Stefano, R. ; Puricelli, S. ; Mediati, M. ; Losciale, L. ; Caputo, D. / Human T-cell lymphotropic virus tax and Epstein-Barr virus DNA in peripheral blood of multiple sclerosis patients during acute attack. In: Acta Neurologica Scandinavica, Supplement. 1997 ; Vol. 95, No. 169. pp. 79-85.
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abstract = "Objectives - A study was performed to determine whether persistent or latent viruses are reactivated during the acute attack in relapsing remitting multiple sclerosis (MS). Material and Methods - DNA of herpes simplex virus type 1 and 2 (HSV-1 and -2), human cytomegalovirus (HCMV), Epstein-Barr virus (EBV), JC virus (JCV) and HTLV-I was searched, using nested polymerase chain reaction (PCR), in peripheral blood mononuclear cells (PBMCs) collected from 14 MS patients on the first day and, twice a week, during an acute attack of the disease. Results - Viral DNA was detected, in at least one PBMC sample, in all the patients. Interestingly, EBV DNA was found in 42.8{\%} of the patients on the first day, while a sharp increase of the HTLV tax-rex DNA frequency (35.7{\%}) was observed on the tenth day. Conclusions - In MS relapse EBV DNA detection is an early, frequent event, while the finding of tax-rex, but not of other HTLV-I genomic regions, is a secondary phenomenon, suggesting that these two factors could interact in the pathogenesis of MS relapses.",
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AU - Caldarelli-Stefano, R.

AU - Puricelli, S.

AU - Mediati, M.

AU - Losciale, L.

AU - Caputo, D.

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AB - Objectives - A study was performed to determine whether persistent or latent viruses are reactivated during the acute attack in relapsing remitting multiple sclerosis (MS). Material and Methods - DNA of herpes simplex virus type 1 and 2 (HSV-1 and -2), human cytomegalovirus (HCMV), Epstein-Barr virus (EBV), JC virus (JCV) and HTLV-I was searched, using nested polymerase chain reaction (PCR), in peripheral blood mononuclear cells (PBMCs) collected from 14 MS patients on the first day and, twice a week, during an acute attack of the disease. Results - Viral DNA was detected, in at least one PBMC sample, in all the patients. Interestingly, EBV DNA was found in 42.8% of the patients on the first day, while a sharp increase of the HTLV tax-rex DNA frequency (35.7%) was observed on the tenth day. Conclusions - In MS relapse EBV DNA detection is an early, frequent event, while the finding of tax-rex, but not of other HTLV-I genomic regions, is a secondary phenomenon, suggesting that these two factors could interact in the pathogenesis of MS relapses.

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