Human T cells expressing the γ/δ T-cell receptor (TcR-1): C(γ)1- and C(γ)2-encoded forms of the receptor correlate with distinctive morphology, cytoskeletal organization, and growth characteristics

C. E. Grossi, E. Ciccone, N. Migone, C. Bottino, D. Zarcone, M. C. Mingari, S. Ferrini, G. Tambussi, O. Viale, G. Casorati, R. Millo, L. Moretta, A. Moretta

Research output: Contribution to journalArticlepeer-review

Abstract

BB3 and δ-TCS1 monoclonal antibodies identify two distinct nonoverlapping populations of T-cell receptor (TcR) γ/δ (TcR-1)-positive cells, which express a disulfide-linked and a nondisulfide-linked form of TcR, respectively. BB3+ cells represented the majority of circulating TcR-1+ cells, but they were virtually undetectable in the thymus. On the other hand, δ-TCS1+ cells were largely predominant among TcR-1+ thymocytes but represented a minority in peripheral blood (PB). Similar distributions were observed by clonal analysis of thymocytes or PB TcR-1+ populations. The use of joining region (J)-specific probes indicated that BB3+ and δ-TCS1+ clones displayed different patterns of J rearrangement. Thus, the disulfide-linked form of TcR-1 (BB3+ clones) was associated with the expression of J segments upstream to the C(γ)1 gene segment, whereas the nondisulfide-linked form (δ-TCS1+ clones) was associated with the expression of J segments upstream to C(γ)2. δ-TCS1+ clones, in most instances, exhibited a growth pattern different from that of BB3+ or conventional TcR α/β+ clones as they adhered promptly to surfaces, spread, and emitted long filopodia ending with adhesion plaques. Ultrastructural analyses showed, exclusively in δ-TCS1+ cells, nuclear deformations, uropod formation, and abundant cytoskeletal structures. In addition, immunofluorescence studies of this subset of TcR-1+ cells revealed the presence of abundant microtubules, intermediate filaments, and submembranous microfilaments. Thus, our findings suggest that δ-TCS1+ cells are capable of active motility.

Original languageEnglish
Pages (from-to)1619-1623
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume86
Issue number5
Publication statusPublished - 1989

ASJC Scopus subject areas

  • General
  • Genetics

Fingerprint

Dive into the research topics of 'Human T cells expressing the γ/δ T-cell receptor (TcR-1): C(γ)1- and C(γ)2-encoded forms of the receptor correlate with distinctive morphology, cytoskeletal organization, and growth characteristics'. Together they form a unique fingerprint.

Cite this