Abstract
Human T cell subpopulations have been defined on the basis of differential expression of either Fc receptors or specfic cell-surface antigens. In this study, we utilized a series of monoclonal antibodies reactive with T cells, monocytes, and Ia antigens to characterize isolated subpopulations of T cells bearing receptors for the Fc portion of IgG (Tγ) and subpopulations of T cells bearing receptors for the Fc portion of IgM Tμ. The results showed that the Tμ population contained both inducer (OKT4 +) and cytotoxic/suppressor (OKT5 +) populations and was similar to the unfractionated T cell population, whereas the Tγ subset contained few T lymphocytes (OKT3 +) and was not enriched for either T cell subset defined by these monoclonal antibodies. Rather, the Tγ population was comprised largely of Ia - cells possessing a monocyte antigen (OKM1 +). In reciprocal studies, it was found that both isolated OKT4 + and OKT5 +T cell subsets contained few Tγ cells, whereas both subsets were mainly comprised of Tμ cells. We conclude that there is little correlation between T cell subsets defined by these monoclonal antibodies and those defined by Fc receptors.
| Original language | English |
|---|---|
| Pages (from-to) | 969-974 |
| Number of pages | 6 |
| Journal | Journal of Experimental Medicine |
| Volume | 151 |
| Issue number | 4 |
| Publication status | Published - 1980 |
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ASJC Scopus subject areas
- Immunology
Cite this
Human T lymphocyte subpopulations defined by Fc receptors and monoclonal antibodies. A comparison. / Reinherz, E. L.; Moretta, L.; Roper, M.; Breard, J. M.; Mingari, M. C.; Cooper, M. D.; Schlossman, S. F.
In: Journal of Experimental Medicine, Vol. 151, No. 4, 1980, p. 969-974.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Human T lymphocyte subpopulations defined by Fc receptors and monoclonal antibodies. A comparison
AU - Reinherz, E. L.
AU - Moretta, L.
AU - Roper, M.
AU - Breard, J. M.
AU - Mingari, M. C.
AU - Cooper, M. D.
AU - Schlossman, S. F.
PY - 1980
Y1 - 1980
N2 - Human T cell subpopulations have been defined on the basis of differential expression of either Fc receptors or specfic cell-surface antigens. In this study, we utilized a series of monoclonal antibodies reactive with T cells, monocytes, and Ia antigens to characterize isolated subpopulations of T cells bearing receptors for the Fc portion of IgG (Tγ) and subpopulations of T cells bearing receptors for the Fc portion of IgM Tμ. The results showed that the Tμ population contained both inducer (OKT4 +) and cytotoxic/suppressor (OKT5 +) populations and was similar to the unfractionated T cell population, whereas the Tγ subset contained few T lymphocytes (OKT3 +) and was not enriched for either T cell subset defined by these monoclonal antibodies. Rather, the Tγ population was comprised largely of Ia - cells possessing a monocyte antigen (OKM1 +). In reciprocal studies, it was found that both isolated OKT4 + and OKT5 +T cell subsets contained few Tγ cells, whereas both subsets were mainly comprised of Tμ cells. We conclude that there is little correlation between T cell subsets defined by these monoclonal antibodies and those defined by Fc receptors.
AB - Human T cell subpopulations have been defined on the basis of differential expression of either Fc receptors or specfic cell-surface antigens. In this study, we utilized a series of monoclonal antibodies reactive with T cells, monocytes, and Ia antigens to characterize isolated subpopulations of T cells bearing receptors for the Fc portion of IgG (Tγ) and subpopulations of T cells bearing receptors for the Fc portion of IgM Tμ. The results showed that the Tμ population contained both inducer (OKT4 +) and cytotoxic/suppressor (OKT5 +) populations and was similar to the unfractionated T cell population, whereas the Tγ subset contained few T lymphocytes (OKT3 +) and was not enriched for either T cell subset defined by these monoclonal antibodies. Rather, the Tγ population was comprised largely of Ia - cells possessing a monocyte antigen (OKM1 +). In reciprocal studies, it was found that both isolated OKT4 + and OKT5 +T cell subsets contained few Tγ cells, whereas both subsets were mainly comprised of Tμ cells. We conclude that there is little correlation between T cell subsets defined by these monoclonal antibodies and those defined by Fc receptors.
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M3 - Article
VL - 151
SP - 969
EP - 974
JO - Journal of Experimental Medicine
JF - Journal of Experimental Medicine
SN - 0022-1007
IS - 4
ER -