Human T lymphocyte subpopulations defined by Fc receptors and monoclonal antibodies. A comparison

E. L. Reinherz, L. Moretta, M. Roper, J. M. Breard, M. C. Mingari, M. D. Cooper, S. F. Schlossman

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Abstract

Human T cell subpopulations have been defined on the basis of differential expression of either Fc receptors or specfic cell-surface antigens. In this study, we utilized a series of monoclonal antibodies reactive with T cells, monocytes, and Ia antigens to characterize isolated subpopulations of T cells bearing receptors for the Fc portion of IgG (Tγ) and subpopulations of T cells bearing receptors for the Fc portion of IgM Tμ. The results showed that the Tμ population contained both inducer (OKT4 +) and cytotoxic/suppressor (OKT5 +) populations and was similar to the unfractionated T cell population, whereas the Tγ subset contained few T lymphocytes (OKT3 +) and was not enriched for either T cell subset defined by these monoclonal antibodies. Rather, the Tγ population was comprised largely of Ia - cells possessing a monocyte antigen (OKM1 +). In reciprocal studies, it was found that both isolated OKT4 + and OKT5 +T cell subsets contained few Tγ cells, whereas both subsets were mainly comprised of Tμ cells. We conclude that there is little correlation between T cell subsets defined by these monoclonal antibodies and those defined by Fc receptors.

Original languageEnglish
Pages (from-to)969-974
Number of pages6
JournalJournal of Experimental Medicine
Volume151
Issue number4
Publication statusPublished - 1980

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Fc Receptors
Lymphocyte Subsets
Monoclonal Antibodies
T-Lymphocytes
T-Lymphocyte Subsets
T-Cell Antigen Receptor
Population
Monocytes
Muromonab-CD3
Histocompatibility Antigens Class II
Surface Antigens
Immunoglobulin M
Immunoglobulin G
Antigens

ASJC Scopus subject areas

  • Immunology

Cite this

Reinherz, E. L., Moretta, L., Roper, M., Breard, J. M., Mingari, M. C., Cooper, M. D., & Schlossman, S. F. (1980). Human T lymphocyte subpopulations defined by Fc receptors and monoclonal antibodies. A comparison. Journal of Experimental Medicine, 151(4), 969-974.

Human T lymphocyte subpopulations defined by Fc receptors and monoclonal antibodies. A comparison. / Reinherz, E. L.; Moretta, L.; Roper, M.; Breard, J. M.; Mingari, M. C.; Cooper, M. D.; Schlossman, S. F.

In: Journal of Experimental Medicine, Vol. 151, No. 4, 1980, p. 969-974.

Research output: Contribution to journalArticle

Reinherz, EL, Moretta, L, Roper, M, Breard, JM, Mingari, MC, Cooper, MD & Schlossman, SF 1980, 'Human T lymphocyte subpopulations defined by Fc receptors and monoclonal antibodies. A comparison', Journal of Experimental Medicine, vol. 151, no. 4, pp. 969-974.
Reinherz, E. L. ; Moretta, L. ; Roper, M. ; Breard, J. M. ; Mingari, M. C. ; Cooper, M. D. ; Schlossman, S. F. / Human T lymphocyte subpopulations defined by Fc receptors and monoclonal antibodies. A comparison. In: Journal of Experimental Medicine. 1980 ; Vol. 151, No. 4. pp. 969-974.
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AU - Mingari, M. C.

AU - Cooper, M. D.

AU - Schlossman, S. F.

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