Abstract
We have analyzed the ability of human γ+/δ+ T cells to recognize a nominal antigen in association with MHC molecules. A TT-specific T cell line with ~40% γ+/δ+ T cells was established from a hyperimmunized donor, D.F., by stimulation with antigen and autologous APC. Three DF-derived γ+/δ+ clones were CD8+ as determined by immunofluorescence staining, and by Southern and Northern blotting with probes detecting δ chain rearrangement and δ and γ chain transcripts, respectively. The γ+/δ+ clones responded to stimulation with TT, but not TNP-BSA, and autologous APC by proliferation and IFN-γ production. No proliferation or IFN-γ production was detected when TT-specific T cell clones were stimulated with either TT or autologous APC only. The response to TT was enhanced by addition of exogenous IL-2. The use of allogeneic APC from 19 donors sharing one HLA-determinant with the autologous donor D.F., showed that the γ+/δ+ T cells responded to TT with HLA-DR4-related restriction as measured by proliferation and IFN-γ production. These results demonstrate that γ/δ receptors can recognize non-MHC-encoded foreign antigen in a self-MHC-restricted fashion.
| Original language | English |
|---|---|
| Pages (from-to) | 1847-1851 |
| Number of pages | 5 |
| Journal | Journal of Experimental Medicine |
| Volume | 169 |
| Issue number | 5 |
| Publication status | Published - 1989 |
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ASJC Scopus subject areas
- Immunology
Cite this
Human TCR-γ+/δ+, CD8+ T lymphocytes recognize tetanus toxoid in an MHC-restricted fashion. / Kozbor, D.; Trinchieri, G.; Monos, D. S.; Isobe, M.; Russo, G.; Haney, J. A.; Zmijewski, C.; Croce, C. M.
In: Journal of Experimental Medicine, Vol. 169, No. 5, 1989, p. 1847-1851.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Human TCR-γ+/δ+, CD8+ T lymphocytes recognize tetanus toxoid in an MHC-restricted fashion
AU - Kozbor, D.
AU - Trinchieri, G.
AU - Monos, D. S.
AU - Isobe, M.
AU - Russo, G.
AU - Haney, J. A.
AU - Zmijewski, C.
AU - Croce, C. M.
PY - 1989
Y1 - 1989
N2 - We have analyzed the ability of human γ+/δ+ T cells to recognize a nominal antigen in association with MHC molecules. A TT-specific T cell line with ~40% γ+/δ+ T cells was established from a hyperimmunized donor, D.F., by stimulation with antigen and autologous APC. Three DF-derived γ+/δ+ clones were CD8+ as determined by immunofluorescence staining, and by Southern and Northern blotting with probes detecting δ chain rearrangement and δ and γ chain transcripts, respectively. The γ+/δ+ clones responded to stimulation with TT, but not TNP-BSA, and autologous APC by proliferation and IFN-γ production. No proliferation or IFN-γ production was detected when TT-specific T cell clones were stimulated with either TT or autologous APC only. The response to TT was enhanced by addition of exogenous IL-2. The use of allogeneic APC from 19 donors sharing one HLA-determinant with the autologous donor D.F., showed that the γ+/δ+ T cells responded to TT with HLA-DR4-related restriction as measured by proliferation and IFN-γ production. These results demonstrate that γ/δ receptors can recognize non-MHC-encoded foreign antigen in a self-MHC-restricted fashion.
AB - We have analyzed the ability of human γ+/δ+ T cells to recognize a nominal antigen in association with MHC molecules. A TT-specific T cell line with ~40% γ+/δ+ T cells was established from a hyperimmunized donor, D.F., by stimulation with antigen and autologous APC. Three DF-derived γ+/δ+ clones were CD8+ as determined by immunofluorescence staining, and by Southern and Northern blotting with probes detecting δ chain rearrangement and δ and γ chain transcripts, respectively. The γ+/δ+ clones responded to stimulation with TT, but not TNP-BSA, and autologous APC by proliferation and IFN-γ production. No proliferation or IFN-γ production was detected when TT-specific T cell clones were stimulated with either TT or autologous APC only. The response to TT was enhanced by addition of exogenous IL-2. The use of allogeneic APC from 19 donors sharing one HLA-determinant with the autologous donor D.F., showed that the γ+/δ+ T cells responded to TT with HLA-DR4-related restriction as measured by proliferation and IFN-γ production. These results demonstrate that γ/δ receptors can recognize non-MHC-encoded foreign antigen in a self-MHC-restricted fashion.
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M3 - Article
C2 - 2469770
AN - SCOPUS:0024596724
VL - 169
SP - 1847
EP - 1851
JO - Journal of Experimental Medicine
JF - Journal of Experimental Medicine
SN - 0022-1007
IS - 5
ER -