Human TCR-γ++, CD8+ T lymphocytes recognize tetanus toxoid in an MHC-restricted fashion

D. Kozbor, G. Trinchieri, D. S. Monos, M. Isobe, G. Russo, J. A. Haney, C. Zmijewski, C. M. Croce

Research output: Contribution to journalArticlepeer-review

Abstract

We have analyzed the ability of human γ++ T cells to recognize a nominal antigen in association with MHC molecules. A TT-specific T cell line with ~40% γ++ T cells was established from a hyperimmunized donor, D.F., by stimulation with antigen and autologous APC. Three DF-derived γ++ clones were CD8+ as determined by immunofluorescence staining, and by Southern and Northern blotting with probes detecting δ chain rearrangement and δ and γ chain transcripts, respectively. The γ++ clones responded to stimulation with TT, but not TNP-BSA, and autologous APC by proliferation and IFN-γ production. No proliferation or IFN-γ production was detected when TT-specific T cell clones were stimulated with either TT or autologous APC only. The response to TT was enhanced by addition of exogenous IL-2. The use of allogeneic APC from 19 donors sharing one HLA-determinant with the autologous donor D.F., showed that the γ++ T cells responded to TT with HLA-DR4-related restriction as measured by proliferation and IFN-γ production. These results demonstrate that γ/δ receptors can recognize non-MHC-encoded foreign antigen in a self-MHC-restricted fashion.

Original languageEnglish
Pages (from-to)1847-1851
Number of pages5
JournalJournal of Experimental Medicine
Volume169
Issue number5
Publication statusPublished - 1989

ASJC Scopus subject areas

  • Immunology

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