Human tenascin gene: Structure of the 5′-region, identification, and characterization of the transcription regulatory sequences

Roberto Gherzi, Barbara Carnemolla, Annalisa Siri, Marco Ponassi, Enrica Balza, Luciano Zardi

Research output: Contribution to journalArticlepeer-review

Abstract

This report describes the genomic organization of the 5′-region of the human tenascin-C (TN) gene and the functional characterization of its promoter. Approximately 2300 base pairs of the TN gene 5′-flanking region have been cloned and sequenced. This genomic region contains several potential binding sites for transcription factors. By primer extension and Sl nuclease analysis we have localized the transcription start site. The first exon of the TN gene (179 base pairs long) is present in the two major TN transcripts, showing that the expression of these two mRNAs is regulated by a single promoter. The 220 bases upstream to the transcription start site are equally active in directing the expression of chloramphenicol acetyltransferase (CAT) reporter gene in TN producer and nonproducer cells. Using deletion fragments of the human 5′-flanking region we have shown the presence of putative "silencer" elements in the -220 to -2300 region active in both TN producer and nonproducer cell lines. Furthermore, we have demonstrated that the selective transcription in TN producing cells requires the presence of a 1.3-kilobase portion of the TN gene intron 1 in the CAT expression vectors. These findings indicate that complex mechanisms control the transcriptional regulation of TN gene.

Original languageEnglish
Pages (from-to)3429-3434
Number of pages6
JournalJournal of Biological Chemistry
Volume270
Issue number7
Publication statusPublished - Feb 17 1995

ASJC Scopus subject areas

  • Biochemistry

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