Human very-low-density lipoprotein receptor complementary DNA and deduced amino acid sequence and localization of its gene (VLDLR) to chromosome band 9p24 by fluorescence in situ hybridization

K. Oka, K. W. Tzung, M. Sullivan, E. Lindsay, A. Baldini, L. Chan

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

A complementary DNA for the very-low-density lipoprotein receptor (VLDLR) that codes for a protein of 873 amino acids was cloned from a human heart cDNA library. The mature protein of 846 amino acids, preceded by a 27- residue signal peptide, shares 97% amino acid sequence identity with the rabbit VLDLR. Like the low-density lipoprotein receptor, the VLDLR contains five different domains, all of which are highly conserved between human and rabbit. A tetrapeptide NPVY that potentially serves as a signal for clustering of the VLDLR on coated pits is present in the cytoplasmic domain, which is 100% conserved between human and rabbit. We localized the VLDLR gene to chromosome 9p24 by fluorescence in situ hybridization using the cloned cDNA as hybridization probe. The high amino acid sequence homology of the VLDLR between two mammalian species suggests that the receptor plays a fundamental role in lipoprotein metabolism and that energy metabolism mediated by triglyceride utilization may be an evolutionarily highly conserved mechanism.

Original languageEnglish
Pages (from-to)298-300
Number of pages3
JournalGenomics
Volume20
Issue number2
DOIs
Publication statusPublished - 1994

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Fluorescence In Situ Hybridization
Amino Acid Sequence
Complementary DNA
Chromosomes
Genes
Rabbits
Amino Acids
Amino Acid Sequence Homology
LDL Receptors
Protein Sorting Signals
Gene Library
Energy Metabolism
Lipoproteins
Cluster Analysis
VLDL receptor
Triglycerides
Proteins

ASJC Scopus subject areas

  • Genetics

Cite this

Human very-low-density lipoprotein receptor complementary DNA and deduced amino acid sequence and localization of its gene (VLDLR) to chromosome band 9p24 by fluorescence in situ hybridization. / Oka, K.; Tzung, K. W.; Sullivan, M.; Lindsay, E.; Baldini, A.; Chan, L.

In: Genomics, Vol. 20, No. 2, 1994, p. 298-300.

Research output: Contribution to journalArticle

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