Humoral and B-cell memory responses in children five years after pertussis acellular vaccine priming

Maria Carollo, Elisabetta Pandolfi, Alberto Eugenio Tozzi, Anne Marie Buisman, Françoise Mascart, Clara Maria Ausiello

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The resurgence of pertussis suggests the need for greater efforts in understanding the long-lasting protective responses induced by vaccination. In this paper we dissect the persistence of humoral and B-cell memory responses induced by primary vaccination with two different acellular pertussis (aP) vaccines, hexavalent Hexavac® vaccine (Hexavac) (Sanofi Pasteur MSD) and Infanrix hexa® (Infanrix) (GlaxoSmithKline Biologicals). We evaluated the specific immune responses in the two groups of children, 5 years after primary vaccination by measuring the persistence of IgG and antibody secreting cells (ASC) specific for vaccine antigens. Part of the enrolled children received only primary vaccination, while others had the pre-school boost dose. A similar level of antigen-specific IgG and ASC was found in Infanrix and Hexavac vaccinated children. The mean IgG levels were significantly higher in children that received the pre-school boost as compared with children that did not receive the boost dose. A longer persistence after the pre-school boost of IgG-Pertussis Toxin (PT) and IgG-pertactin levels was observed in Infanrix primed children, but it was not statistically significant. More than 80% of children presented a positive ASC B memory response. Around 50% of children still presented protective IgG-PT levels which are reduced to 36% in no-boosted children. The pre-school booster dose restores the percentage of protected children above 50%. In conclusion our data underline the importance of giving a booster dose 5 years after primary vaccination and suggest the need for a new vaccine able to induce a long lasting protective response.

Original languageEnglish
Pages (from-to)2093-2099
Number of pages7
JournalVaccine
Volume32
Issue number18
DOIs
Publication statusPublished - Apr 11 2014

Fingerprint

Acellular Vaccines
Pertussis Vaccine
B-lymphocytes
B-Lymphocytes
vaccines
Diphtheria-Tetanus-acellular Pertussis Vaccines
Immunoglobulin G
Vaccination
Antibody-Producing Cells
vaccination
pertussis toxin
Vaccines
Pertussis Toxin
dosage
antibodies
antigens
Antigens
Whooping Cough
immune response

Keywords

  • Antibodies
  • Booster vaccination
  • Children
  • Memory B-cell immune response
  • Pertussis
  • Vaccination

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)

Cite this

Humoral and B-cell memory responses in children five years after pertussis acellular vaccine priming. / Carollo, Maria; Pandolfi, Elisabetta; Tozzi, Alberto Eugenio; Buisman, Anne Marie; Mascart, Françoise; Ausiello, Clara Maria.

In: Vaccine, Vol. 32, No. 18, 11.04.2014, p. 2093-2099.

Research output: Contribution to journalArticle

Carollo, Maria ; Pandolfi, Elisabetta ; Tozzi, Alberto Eugenio ; Buisman, Anne Marie ; Mascart, Françoise ; Ausiello, Clara Maria. / Humoral and B-cell memory responses in children five years after pertussis acellular vaccine priming. In: Vaccine. 2014 ; Vol. 32, No. 18. pp. 2093-2099.
@article{61607e04bef44ab69f0c9e3355601341,
title = "Humoral and B-cell memory responses in children five years after pertussis acellular vaccine priming",
abstract = "The resurgence of pertussis suggests the need for greater efforts in understanding the long-lasting protective responses induced by vaccination. In this paper we dissect the persistence of humoral and B-cell memory responses induced by primary vaccination with two different acellular pertussis (aP) vaccines, hexavalent Hexavac{\circledR} vaccine (Hexavac) (Sanofi Pasteur MSD) and Infanrix hexa{\circledR} (Infanrix) (GlaxoSmithKline Biologicals). We evaluated the specific immune responses in the two groups of children, 5 years after primary vaccination by measuring the persistence of IgG and antibody secreting cells (ASC) specific for vaccine antigens. Part of the enrolled children received only primary vaccination, while others had the pre-school boost dose. A similar level of antigen-specific IgG and ASC was found in Infanrix and Hexavac vaccinated children. The mean IgG levels were significantly higher in children that received the pre-school boost as compared with children that did not receive the boost dose. A longer persistence after the pre-school boost of IgG-Pertussis Toxin (PT) and IgG-pertactin levels was observed in Infanrix primed children, but it was not statistically significant. More than 80{\%} of children presented a positive ASC B memory response. Around 50{\%} of children still presented protective IgG-PT levels which are reduced to 36{\%} in no-boosted children. The pre-school booster dose restores the percentage of protected children above 50{\%}. In conclusion our data underline the importance of giving a booster dose 5 years after primary vaccination and suggest the need for a new vaccine able to induce a long lasting protective response.",
keywords = "Antibodies, Booster vaccination, Children, Memory B-cell immune response, Pertussis, Vaccination",
author = "Maria Carollo and Elisabetta Pandolfi and Tozzi, {Alberto Eugenio} and Buisman, {Anne Marie} and Fran{\cc}oise Mascart and Ausiello, {Clara Maria}",
year = "2014",
month = "4",
day = "11",
doi = "10.1016/j.vaccine.2014.02.005",
language = "English",
volume = "32",
pages = "2093--2099",
journal = "Vaccine",
issn = "0264-410X",
publisher = "Elsevier BV",
number = "18",

}

TY - JOUR

T1 - Humoral and B-cell memory responses in children five years after pertussis acellular vaccine priming

AU - Carollo, Maria

AU - Pandolfi, Elisabetta

AU - Tozzi, Alberto Eugenio

AU - Buisman, Anne Marie

AU - Mascart, Françoise

AU - Ausiello, Clara Maria

PY - 2014/4/11

Y1 - 2014/4/11

N2 - The resurgence of pertussis suggests the need for greater efforts in understanding the long-lasting protective responses induced by vaccination. In this paper we dissect the persistence of humoral and B-cell memory responses induced by primary vaccination with two different acellular pertussis (aP) vaccines, hexavalent Hexavac® vaccine (Hexavac) (Sanofi Pasteur MSD) and Infanrix hexa® (Infanrix) (GlaxoSmithKline Biologicals). We evaluated the specific immune responses in the two groups of children, 5 years after primary vaccination by measuring the persistence of IgG and antibody secreting cells (ASC) specific for vaccine antigens. Part of the enrolled children received only primary vaccination, while others had the pre-school boost dose. A similar level of antigen-specific IgG and ASC was found in Infanrix and Hexavac vaccinated children. The mean IgG levels were significantly higher in children that received the pre-school boost as compared with children that did not receive the boost dose. A longer persistence after the pre-school boost of IgG-Pertussis Toxin (PT) and IgG-pertactin levels was observed in Infanrix primed children, but it was not statistically significant. More than 80% of children presented a positive ASC B memory response. Around 50% of children still presented protective IgG-PT levels which are reduced to 36% in no-boosted children. The pre-school booster dose restores the percentage of protected children above 50%. In conclusion our data underline the importance of giving a booster dose 5 years after primary vaccination and suggest the need for a new vaccine able to induce a long lasting protective response.

AB - The resurgence of pertussis suggests the need for greater efforts in understanding the long-lasting protective responses induced by vaccination. In this paper we dissect the persistence of humoral and B-cell memory responses induced by primary vaccination with two different acellular pertussis (aP) vaccines, hexavalent Hexavac® vaccine (Hexavac) (Sanofi Pasteur MSD) and Infanrix hexa® (Infanrix) (GlaxoSmithKline Biologicals). We evaluated the specific immune responses in the two groups of children, 5 years after primary vaccination by measuring the persistence of IgG and antibody secreting cells (ASC) specific for vaccine antigens. Part of the enrolled children received only primary vaccination, while others had the pre-school boost dose. A similar level of antigen-specific IgG and ASC was found in Infanrix and Hexavac vaccinated children. The mean IgG levels were significantly higher in children that received the pre-school boost as compared with children that did not receive the boost dose. A longer persistence after the pre-school boost of IgG-Pertussis Toxin (PT) and IgG-pertactin levels was observed in Infanrix primed children, but it was not statistically significant. More than 80% of children presented a positive ASC B memory response. Around 50% of children still presented protective IgG-PT levels which are reduced to 36% in no-boosted children. The pre-school booster dose restores the percentage of protected children above 50%. In conclusion our data underline the importance of giving a booster dose 5 years after primary vaccination and suggest the need for a new vaccine able to induce a long lasting protective response.

KW - Antibodies

KW - Booster vaccination

KW - Children

KW - Memory B-cell immune response

KW - Pertussis

KW - Vaccination

UR - http://www.scopus.com/inward/record.url?scp=84896544462&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84896544462&partnerID=8YFLogxK

U2 - 10.1016/j.vaccine.2014.02.005

DO - 10.1016/j.vaccine.2014.02.005

M3 - Article

VL - 32

SP - 2093

EP - 2099

JO - Vaccine

JF - Vaccine

SN - 0264-410X

IS - 18

ER -