HuR interacts with lincBRN1a and lincBRN1b during neuronal stem cells differentiation

Stephana Carelli, Toniella Giallongo, Federica Rey, Elisa Latorre, Matteo Bordoni, Serena Mazzucchelli, Maria Carlotta Gorio, Orietta Pansarasa, Alessandro Provenzani, Cristina Cereda, Anna Maria Di Giulio

Research output: Contribution to journalArticle

Abstract

LncRNAs play crucial roles in cellular processes and their regulatory effects in the adult brain and neural stem cells (NSCs) remain to be entirely characterized. We report that 10 lncRNAs (LincENC1, FABL, lincp21, HAUNT, PERIL, lincBRN1a, lincBRN1b, HOTTIP, TUG1 and FENDRR) are expressed during murine NSCs differentiation and interact with the RNA-binding protein ELAVL1/HuR. Furthermore, we characterize the function of two of the deregulated lncRNAs, lincBRN1a and lincBRN1b, during NSCs' differentiation. Their inhibition leads to the induction of differentiation, with a concomitant decrease in stemness and an increase in neuronal markers, indicating that they exert key functions in neuronal cells differentiation. Furthermore, we describe here that HuR regulates their half-life, suggesting their synergic role in the differentiation process. We also identify six human homologs (PANTR1, TUG1, HOTTIP, TP53COR, ELDRR and FENDRR) of the mentioned 10 lncRNAs and we report their deregulation during human iPSCs differentiation into neurons. In conclusion, our results strongly indicate a key synergic role for lncRNAs and HuR in neuronal stem cells fate.

Original languageEnglish
Pages (from-to)1471-85
Number of pages15
JournalRNA Biology
Volume 16
Issue number(10)
Early online dateJul 26 2019
DOIs
Publication statusPublished - 2019

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Long Noncoding RNA
Neural Stem Cells
Cell Differentiation
Stem Cells
Adult Stem Cells
RNA-Binding Proteins
Brain Stem
Half-Life
Neurons

Cite this

Carelli, S., Giallongo, T., Rey, F., Latorre, E., Bordoni, M., Mazzucchelli, S., ... Di Giulio, A. M. (2019). HuR interacts with lincBRN1a and lincBRN1b during neuronal stem cells differentiation. RNA Biology, 16 ((10)), 1471-85. https://doi.org/10.1080/15476286.2019.1637698

HuR interacts with lincBRN1a and lincBRN1b during neuronal stem cells differentiation. / Carelli, Stephana; Giallongo, Toniella; Rey, Federica; Latorre, Elisa; Bordoni, Matteo; Mazzucchelli, Serena; Gorio, Maria Carlotta; Pansarasa, Orietta; Provenzani, Alessandro; Cereda, Cristina; Di Giulio, Anna Maria.

In: RNA Biology, Vol. 16 , No. (10), 2019, p. 1471-85.

Research output: Contribution to journalArticle

Carelli, S, Giallongo, T, Rey, F, Latorre, E, Bordoni, M, Mazzucchelli, S, Gorio, MC, Pansarasa, O, Provenzani, A, Cereda, C & Di Giulio, AM 2019, 'HuR interacts with lincBRN1a and lincBRN1b during neuronal stem cells differentiation', RNA Biology, vol. 16 , no. (10), pp. 1471-85. https://doi.org/10.1080/15476286.2019.1637698
Carelli, Stephana ; Giallongo, Toniella ; Rey, Federica ; Latorre, Elisa ; Bordoni, Matteo ; Mazzucchelli, Serena ; Gorio, Maria Carlotta ; Pansarasa, Orietta ; Provenzani, Alessandro ; Cereda, Cristina ; Di Giulio, Anna Maria. / HuR interacts with lincBRN1a and lincBRN1b during neuronal stem cells differentiation. In: RNA Biology. 2019 ; Vol. 16 , No. (10). pp. 1471-85.
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abstract = "LncRNAs play crucial roles in cellular processes and their regulatory effects in the adult brain and neural stem cells (NSCs) remain to be entirely characterized. We report that 10 lncRNAs (LincENC1, FABL, lincp21, HAUNT, PERIL, lincBRN1a, lincBRN1b, HOTTIP, TUG1 and FENDRR) are expressed during murine NSCs differentiation and interact with the RNA-binding protein ELAVL1/HuR. Furthermore, we characterize the function of two of the deregulated lncRNAs, lincBRN1a and lincBRN1b, during NSCs' differentiation. Their inhibition leads to the induction of differentiation, with a concomitant decrease in stemness and an increase in neuronal markers, indicating that they exert key functions in neuronal cells differentiation. Furthermore, we describe here that HuR regulates their half-life, suggesting their synergic role in the differentiation process. We also identify six human homologs (PANTR1, TUG1, HOTTIP, TP53COR, ELDRR and FENDRR) of the mentioned 10 lncRNAs and we report their deregulation during human iPSCs differentiation into neurons. In conclusion, our results strongly indicate a key synergic role for lncRNAs and HuR in neuronal stem cells fate.",
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AU - Bordoni, Matteo

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AB - LncRNAs play crucial roles in cellular processes and their regulatory effects in the adult brain and neural stem cells (NSCs) remain to be entirely characterized. We report that 10 lncRNAs (LincENC1, FABL, lincp21, HAUNT, PERIL, lincBRN1a, lincBRN1b, HOTTIP, TUG1 and FENDRR) are expressed during murine NSCs differentiation and interact with the RNA-binding protein ELAVL1/HuR. Furthermore, we characterize the function of two of the deregulated lncRNAs, lincBRN1a and lincBRN1b, during NSCs' differentiation. Their inhibition leads to the induction of differentiation, with a concomitant decrease in stemness and an increase in neuronal markers, indicating that they exert key functions in neuronal cells differentiation. Furthermore, we describe here that HuR regulates their half-life, suggesting their synergic role in the differentiation process. We also identify six human homologs (PANTR1, TUG1, HOTTIP, TP53COR, ELDRR and FENDRR) of the mentioned 10 lncRNAs and we report their deregulation during human iPSCs differentiation into neurons. In conclusion, our results strongly indicate a key synergic role for lncRNAs and HuR in neuronal stem cells fate.

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