Abstract
LncRNAs play crucial roles in cellular processes and their regulatory effects in the adult brain and neural stem cells (NSCs) remain to be entirely characterized. We report that 10 lncRNAs (LincENC1, FABL, lincp21, HAUNT, PERIL, lincBRN1a, lincBRN1b, HOTTIP, TUG1 and FENDRR) are expressed during murine NSCs differentiation and interact with the RNA-binding protein ELAVL1/HuR. Furthermore, we characterize the function of two of the deregulated lncRNAs, lincBRN1a and lincBRN1b, during NSCs' differentiation. Their inhibition leads to the induction of differentiation, with a concomitant decrease in stemness and an increase in neuronal markers, indicating that they exert key functions in neuronal cells differentiation. Furthermore, we describe here that HuR regulates their half-life, suggesting their synergic role in the differentiation process. We also identify six human homologs (PANTR1, TUG1, HOTTIP, TP53COR, ELDRR and FENDRR) of the mentioned 10 lncRNAs and we report their deregulation during human iPSCs differentiation into neurons. In conclusion, our results strongly indicate a key synergic role for lncRNAs and HuR in neuronal stem cells fate.
| Original language | English |
|---|---|
| Pages (from-to) | 1471-85 |
| Number of pages | 15 |
| Journal | RNA Biology |
| Volume | 16 |
| Issue number | (10) |
| Early online date | Jul 26 2019 |
| DOIs | |
| Publication status | Published - 2019 |
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HuR interacts with lincBRN1a and lincBRN1b during neuronal stem cells differentiation. / Carelli, Stephana; Giallongo, Toniella; Rey, Federica; Latorre, Elisa; Bordoni, Matteo; Mazzucchelli, Serena; Gorio, Maria Carlotta; Pansarasa, Orietta; Provenzani, Alessandro; Cereda, Cristina; Di Giulio, Anna Maria.
In: RNA Biology, Vol. 16 , No. (10), 2019, p. 1471-85.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - HuR interacts with lincBRN1a and lincBRN1b during neuronal stem cells differentiation
AU - Carelli, Stephana
AU - Giallongo, Toniella
AU - Rey, Federica
AU - Latorre, Elisa
AU - Bordoni, Matteo
AU - Mazzucchelli, Serena
AU - Gorio, Maria Carlotta
AU - Pansarasa, Orietta
AU - Provenzani, Alessandro
AU - Cereda, Cristina
AU - Di Giulio, Anna Maria
PY - 2019
Y1 - 2019
N2 - LncRNAs play crucial roles in cellular processes and their regulatory effects in the adult brain and neural stem cells (NSCs) remain to be entirely characterized. We report that 10 lncRNAs (LincENC1, FABL, lincp21, HAUNT, PERIL, lincBRN1a, lincBRN1b, HOTTIP, TUG1 and FENDRR) are expressed during murine NSCs differentiation and interact with the RNA-binding protein ELAVL1/HuR. Furthermore, we characterize the function of two of the deregulated lncRNAs, lincBRN1a and lincBRN1b, during NSCs' differentiation. Their inhibition leads to the induction of differentiation, with a concomitant decrease in stemness and an increase in neuronal markers, indicating that they exert key functions in neuronal cells differentiation. Furthermore, we describe here that HuR regulates their half-life, suggesting their synergic role in the differentiation process. We also identify six human homologs (PANTR1, TUG1, HOTTIP, TP53COR, ELDRR and FENDRR) of the mentioned 10 lncRNAs and we report their deregulation during human iPSCs differentiation into neurons. In conclusion, our results strongly indicate a key synergic role for lncRNAs and HuR in neuronal stem cells fate.
AB - LncRNAs play crucial roles in cellular processes and their regulatory effects in the adult brain and neural stem cells (NSCs) remain to be entirely characterized. We report that 10 lncRNAs (LincENC1, FABL, lincp21, HAUNT, PERIL, lincBRN1a, lincBRN1b, HOTTIP, TUG1 and FENDRR) are expressed during murine NSCs differentiation and interact with the RNA-binding protein ELAVL1/HuR. Furthermore, we characterize the function of two of the deregulated lncRNAs, lincBRN1a and lincBRN1b, during NSCs' differentiation. Their inhibition leads to the induction of differentiation, with a concomitant decrease in stemness and an increase in neuronal markers, indicating that they exert key functions in neuronal cells differentiation. Furthermore, we describe here that HuR regulates their half-life, suggesting their synergic role in the differentiation process. We also identify six human homologs (PANTR1, TUG1, HOTTIP, TP53COR, ELDRR and FENDRR) of the mentioned 10 lncRNAs and we report their deregulation during human iPSCs differentiation into neurons. In conclusion, our results strongly indicate a key synergic role for lncRNAs and HuR in neuronal stem cells fate.
U2 - 10.1080/15476286.2019.1637698
DO - 10.1080/15476286.2019.1637698
M3 - Article
C2 - 31345103
VL - 16
SP - 1471
EP - 1485
JO - RNA Biology
JF - RNA Biology
SN - 1547-6286
IS - (10)
ER -