Hyaluronan-based pericellular matrix: Substrate electrostatic charges and early cell adhesion events

Caterina Fotia, Grazia M L Messina, Giovanni Marletta, Nicola Baldini, Gabriela Ciapetti

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Cells are surrounded by a hyaluronan-rich coat called 'pericellular matrix' (PCM), mainly constituted by hyaluronan, a long-chain linear polysaccharide which is secreted and resorbed by the cell, depending on its activity. Cell attachment to a surface is mediated by PCM before integrins and focal adhesions are involved. As hyaluronan is known to bear a negative charge at physiological pH, the relevance of its electrical properties in driving the early cell adhesion steps has been studied, exploring how PCM mediates cell adhesion to charged surfaces, such as polyelectrolyte multilayer (PEM) films. Poly(ethylene imine) (PEI) and poly(sodium 4-styrene sulphonate) (PSS), assembled as PEI/PSS and PEI/PSS/PEI layers, were used. The nanoscale morphology of such layers was analysed by atomic force microscopy, and the detailed surface structure was analysed by X-ray photoemission spectroscopy. PCMcoated and PCM-depleted MG63 osteoblast-like cells were used, and cell density, morphology and adhesive structures were analysed during early steps of cell attachment to the PEM surfaces (1-6 h). The present study demonstrates that the pericellular matrix is involved in cell adhesion to material surfaces, and its arrangement depends on the cell interaction with the surface. Moreover, the PCM/surface interaction is not simply driven by electrostatic effects, as the cell response may be affected by specific chemical groups at the material surface. In the development of biomimetic surfaces promoting cell adhesion and function, the role of this unrecognised outer cell structure has to be taken into account.

Original languageEnglish
Pages (from-to)133-149
Number of pages17
JournalEuropean Cells and Materials
Volume26
Publication statusPublished - Sep 20 2013

Fingerprint

Cell adhesion
Hyaluronic Acid
Static Electricity
Cell Adhesion
Electrostatics
Polyetherimides
Substrates
Polyelectrolytes
Cell-Matrix Junctions
Photoelectron Spectroscopy
Biomimetics
Focal Adhesions
Atomic Force Microscopy
Osteoblasts
Multilayer films
Integrins
Cell Communication
Adhesives
Polysaccharides
Photoelectron spectroscopy

Keywords

  • Cell adhesion
  • Hyaluronan
  • Pericellular matrix
  • Polyelectrolyte multilayers

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Bioengineering
  • Biomedical Engineering
  • Biomaterials

Cite this

Hyaluronan-based pericellular matrix : Substrate electrostatic charges and early cell adhesion events. / Fotia, Caterina; Messina, Grazia M L; Marletta, Giovanni; Baldini, Nicola; Ciapetti, Gabriela.

In: European Cells and Materials, Vol. 26, 20.09.2013, p. 133-149.

Research output: Contribution to journalArticle

@article{ef1d4556a68a4d4c8db502150869080f,
title = "Hyaluronan-based pericellular matrix: Substrate electrostatic charges and early cell adhesion events",
abstract = "Cells are surrounded by a hyaluronan-rich coat called 'pericellular matrix' (PCM), mainly constituted by hyaluronan, a long-chain linear polysaccharide which is secreted and resorbed by the cell, depending on its activity. Cell attachment to a surface is mediated by PCM before integrins and focal adhesions are involved. As hyaluronan is known to bear a negative charge at physiological pH, the relevance of its electrical properties in driving the early cell adhesion steps has been studied, exploring how PCM mediates cell adhesion to charged surfaces, such as polyelectrolyte multilayer (PEM) films. Poly(ethylene imine) (PEI) and poly(sodium 4-styrene sulphonate) (PSS), assembled as PEI/PSS and PEI/PSS/PEI layers, were used. The nanoscale morphology of such layers was analysed by atomic force microscopy, and the detailed surface structure was analysed by X-ray photoemission spectroscopy. PCMcoated and PCM-depleted MG63 osteoblast-like cells were used, and cell density, morphology and adhesive structures were analysed during early steps of cell attachment to the PEM surfaces (1-6 h). The present study demonstrates that the pericellular matrix is involved in cell adhesion to material surfaces, and its arrangement depends on the cell interaction with the surface. Moreover, the PCM/surface interaction is not simply driven by electrostatic effects, as the cell response may be affected by specific chemical groups at the material surface. In the development of biomimetic surfaces promoting cell adhesion and function, the role of this unrecognised outer cell structure has to be taken into account.",
keywords = "Cell adhesion, Hyaluronan, Pericellular matrix, Polyelectrolyte multilayers",
author = "Caterina Fotia and Messina, {Grazia M L} and Giovanni Marletta and Nicola Baldini and Gabriela Ciapetti",
year = "2013",
month = "9",
day = "20",
language = "English",
volume = "26",
pages = "133--149",
journal = "European Cells and Materials",
issn = "1473-2262",
publisher = "Swiss Society for Biomaterials",

}

TY - JOUR

T1 - Hyaluronan-based pericellular matrix

T2 - Substrate electrostatic charges and early cell adhesion events

AU - Fotia, Caterina

AU - Messina, Grazia M L

AU - Marletta, Giovanni

AU - Baldini, Nicola

AU - Ciapetti, Gabriela

PY - 2013/9/20

Y1 - 2013/9/20

N2 - Cells are surrounded by a hyaluronan-rich coat called 'pericellular matrix' (PCM), mainly constituted by hyaluronan, a long-chain linear polysaccharide which is secreted and resorbed by the cell, depending on its activity. Cell attachment to a surface is mediated by PCM before integrins and focal adhesions are involved. As hyaluronan is known to bear a negative charge at physiological pH, the relevance of its electrical properties in driving the early cell adhesion steps has been studied, exploring how PCM mediates cell adhesion to charged surfaces, such as polyelectrolyte multilayer (PEM) films. Poly(ethylene imine) (PEI) and poly(sodium 4-styrene sulphonate) (PSS), assembled as PEI/PSS and PEI/PSS/PEI layers, were used. The nanoscale morphology of such layers was analysed by atomic force microscopy, and the detailed surface structure was analysed by X-ray photoemission spectroscopy. PCMcoated and PCM-depleted MG63 osteoblast-like cells were used, and cell density, morphology and adhesive structures were analysed during early steps of cell attachment to the PEM surfaces (1-6 h). The present study demonstrates that the pericellular matrix is involved in cell adhesion to material surfaces, and its arrangement depends on the cell interaction with the surface. Moreover, the PCM/surface interaction is not simply driven by electrostatic effects, as the cell response may be affected by specific chemical groups at the material surface. In the development of biomimetic surfaces promoting cell adhesion and function, the role of this unrecognised outer cell structure has to be taken into account.

AB - Cells are surrounded by a hyaluronan-rich coat called 'pericellular matrix' (PCM), mainly constituted by hyaluronan, a long-chain linear polysaccharide which is secreted and resorbed by the cell, depending on its activity. Cell attachment to a surface is mediated by PCM before integrins and focal adhesions are involved. As hyaluronan is known to bear a negative charge at physiological pH, the relevance of its electrical properties in driving the early cell adhesion steps has been studied, exploring how PCM mediates cell adhesion to charged surfaces, such as polyelectrolyte multilayer (PEM) films. Poly(ethylene imine) (PEI) and poly(sodium 4-styrene sulphonate) (PSS), assembled as PEI/PSS and PEI/PSS/PEI layers, were used. The nanoscale morphology of such layers was analysed by atomic force microscopy, and the detailed surface structure was analysed by X-ray photoemission spectroscopy. PCMcoated and PCM-depleted MG63 osteoblast-like cells were used, and cell density, morphology and adhesive structures were analysed during early steps of cell attachment to the PEM surfaces (1-6 h). The present study demonstrates that the pericellular matrix is involved in cell adhesion to material surfaces, and its arrangement depends on the cell interaction with the surface. Moreover, the PCM/surface interaction is not simply driven by electrostatic effects, as the cell response may be affected by specific chemical groups at the material surface. In the development of biomimetic surfaces promoting cell adhesion and function, the role of this unrecognised outer cell structure has to be taken into account.

KW - Cell adhesion

KW - Hyaluronan

KW - Pericellular matrix

KW - Polyelectrolyte multilayers

UR - http://www.scopus.com/inward/record.url?scp=84884683059&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84884683059&partnerID=8YFLogxK

M3 - Article

C2 - 24052426

AN - SCOPUS:84884683059

VL - 26

SP - 133

EP - 149

JO - European Cells and Materials

JF - European Cells and Materials

SN - 1473-2262

ER -