Abstract
In order to overcome the problem of rapid clearance of the polysaccharide hyaluronic acid (Hyal) in the treatment of osteoarthritis (OA), a 50% cross-linked Hyal hydrogel (Hyal 50%) was synthesised. The 50% refers to the amount of COOH groups of the polysaccharide involved in the cross-linking reaction, i.e. 50% of the total amount. The rheological behaviour of the Hyal 50% hydrogel, and in particular the possibility to inject it through a needle, was studied. The results obtained demonstrated that the hydrogel injected through the needle still behaved like a gel, although it showed a reduction of the dynamic moduli. The most appropriate sterilisation technique for this kind of hydrogel was also evaluated. Liophilised Hyal 50% samples were sterilised by steam, Ethylene Oxide (EtO) and γ-rays. EtO and γ-rays did not modify the characteristics of the hydrogel in terms of swellability and morphology. Lastly, the in vivo effect of Hyal 50% hydrogel in the treatment of chondral defect in rabbit knee was also studied. The results obtained showed the Hyal 50% injections improved chondrocytes density and matrix appearance. Furthermore, the permanence in situ of the hydrogel was longer than that of the linear Hyal.
| Original language | English |
|---|---|
| Pages (from-to) | 4503-4513 |
| Number of pages | 11 |
| Journal | Biomaterials |
| Volume | 23 |
| Issue number | 23 |
| DOIs | |
| Publication status | Published - Dec 2002 |
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Keywords
- Hyaluronan hydrogel
- In vivo tests
- Osteoarthritis
- Rheological properties
- Sterilisation
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Biomedical Engineering
Cite this
Hyaluronic acid hydrogel in the treatment of osteoarthritis. / Barbucci, R.; Lamponi, S.; Borzacchiello, A.; Ambrosio, L.; Fini, M.; Torricelli, P.; Giardino, R.
In: Biomaterials, Vol. 23, No. 23, 12.2002, p. 4503-4513.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Hyaluronic acid hydrogel in the treatment of osteoarthritis
AU - Barbucci, R.
AU - Lamponi, S.
AU - Borzacchiello, A.
AU - Ambrosio, L.
AU - Fini, M.
AU - Torricelli, P.
AU - Giardino, R.
PY - 2002/12
Y1 - 2002/12
N2 - In order to overcome the problem of rapid clearance of the polysaccharide hyaluronic acid (Hyal) in the treatment of osteoarthritis (OA), a 50% cross-linked Hyal hydrogel (Hyal 50%) was synthesised. The 50% refers to the amount of COOH groups of the polysaccharide involved in the cross-linking reaction, i.e. 50% of the total amount. The rheological behaviour of the Hyal 50% hydrogel, and in particular the possibility to inject it through a needle, was studied. The results obtained demonstrated that the hydrogel injected through the needle still behaved like a gel, although it showed a reduction of the dynamic moduli. The most appropriate sterilisation technique for this kind of hydrogel was also evaluated. Liophilised Hyal 50% samples were sterilised by steam, Ethylene Oxide (EtO) and γ-rays. EtO and γ-rays did not modify the characteristics of the hydrogel in terms of swellability and morphology. Lastly, the in vivo effect of Hyal 50% hydrogel in the treatment of chondral defect in rabbit knee was also studied. The results obtained showed the Hyal 50% injections improved chondrocytes density and matrix appearance. Furthermore, the permanence in situ of the hydrogel was longer than that of the linear Hyal.
AB - In order to overcome the problem of rapid clearance of the polysaccharide hyaluronic acid (Hyal) in the treatment of osteoarthritis (OA), a 50% cross-linked Hyal hydrogel (Hyal 50%) was synthesised. The 50% refers to the amount of COOH groups of the polysaccharide involved in the cross-linking reaction, i.e. 50% of the total amount. The rheological behaviour of the Hyal 50% hydrogel, and in particular the possibility to inject it through a needle, was studied. The results obtained demonstrated that the hydrogel injected through the needle still behaved like a gel, although it showed a reduction of the dynamic moduli. The most appropriate sterilisation technique for this kind of hydrogel was also evaluated. Liophilised Hyal 50% samples were sterilised by steam, Ethylene Oxide (EtO) and γ-rays. EtO and γ-rays did not modify the characteristics of the hydrogel in terms of swellability and morphology. Lastly, the in vivo effect of Hyal 50% hydrogel in the treatment of chondral defect in rabbit knee was also studied. The results obtained showed the Hyal 50% injections improved chondrocytes density and matrix appearance. Furthermore, the permanence in situ of the hydrogel was longer than that of the linear Hyal.
KW - Hyaluronan hydrogel
KW - In vivo tests
KW - Osteoarthritis
KW - Rheological properties
KW - Sterilisation
UR - http://www.scopus.com/inward/record.url?scp=0036968183&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036968183&partnerID=8YFLogxK
U2 - 10.1016/S0142-9612(02)00194-1
DO - 10.1016/S0142-9612(02)00194-1
M3 - Article
C2 - 12322970
AN - SCOPUS:0036968183
VL - 23
SP - 4503
EP - 4513
JO - Biomaterials
JF - Biomaterials
SN - 0142-9612
IS - 23
ER -