Hyaluronic acid hydrogel in the treatment of osteoarthritis

R. Barbucci, S. Lamponi, A. Borzacchiello, L. Ambrosio, M. Fini, P. Torricelli, R. Giardino

Research output: Contribution to journalArticle

124 Citations (Scopus)

Abstract

In order to overcome the problem of rapid clearance of the polysaccharide hyaluronic acid (Hyal) in the treatment of osteoarthritis (OA), a 50% cross-linked Hyal hydrogel (Hyal 50%) was synthesised. The 50% refers to the amount of COOH groups of the polysaccharide involved in the cross-linking reaction, i.e. 50% of the total amount. The rheological behaviour of the Hyal 50% hydrogel, and in particular the possibility to inject it through a needle, was studied. The results obtained demonstrated that the hydrogel injected through the needle still behaved like a gel, although it showed a reduction of the dynamic moduli. The most appropriate sterilisation technique for this kind of hydrogel was also evaluated. Liophilised Hyal 50% samples were sterilised by steam, Ethylene Oxide (EtO) and γ-rays. EtO and γ-rays did not modify the characteristics of the hydrogel in terms of swellability and morphology. Lastly, the in vivo effect of Hyal 50% hydrogel in the treatment of chondral defect in rabbit knee was also studied. The results obtained showed the Hyal 50% injections improved chondrocytes density and matrix appearance. Furthermore, the permanence in situ of the hydrogel was longer than that of the linear Hyal.

Original languageEnglish
Pages (from-to)4503-4513
Number of pages11
JournalBiomaterials
Volume23
Issue number23
DOIs
Publication statusPublished - Dec 2002

Fingerprint

Hyaluronic acid
Hydrogel
Hyaluronic Acid
Hydrogels
Osteoarthritis
Ethylene Oxide
Therapeutics
Polysaccharides
Needles
Ethylene
Oxides
Cross Reactions
Steam
Chondrocytes
Cartilage
Knee
Gels
Rabbits
Defects
Injections

Keywords

  • Hyaluronan hydrogel
  • In vivo tests
  • Osteoarthritis
  • Rheological properties
  • Sterilisation

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

Cite this

Barbucci, R., Lamponi, S., Borzacchiello, A., Ambrosio, L., Fini, M., Torricelli, P., & Giardino, R. (2002). Hyaluronic acid hydrogel in the treatment of osteoarthritis. Biomaterials, 23(23), 4503-4513. https://doi.org/10.1016/S0142-9612(02)00194-1

Hyaluronic acid hydrogel in the treatment of osteoarthritis. / Barbucci, R.; Lamponi, S.; Borzacchiello, A.; Ambrosio, L.; Fini, M.; Torricelli, P.; Giardino, R.

In: Biomaterials, Vol. 23, No. 23, 12.2002, p. 4503-4513.

Research output: Contribution to journalArticle

Barbucci, R, Lamponi, S, Borzacchiello, A, Ambrosio, L, Fini, M, Torricelli, P & Giardino, R 2002, 'Hyaluronic acid hydrogel in the treatment of osteoarthritis', Biomaterials, vol. 23, no. 23, pp. 4503-4513. https://doi.org/10.1016/S0142-9612(02)00194-1
Barbucci, R. ; Lamponi, S. ; Borzacchiello, A. ; Ambrosio, L. ; Fini, M. ; Torricelli, P. ; Giardino, R. / Hyaluronic acid hydrogel in the treatment of osteoarthritis. In: Biomaterials. 2002 ; Vol. 23, No. 23. pp. 4503-4513.
@article{4845fc51b9a245d7b4724bf702ed42e7,
title = "Hyaluronic acid hydrogel in the treatment of osteoarthritis",
abstract = "In order to overcome the problem of rapid clearance of the polysaccharide hyaluronic acid (Hyal) in the treatment of osteoarthritis (OA), a 50{\%} cross-linked Hyal hydrogel (Hyal 50{\%}) was synthesised. The 50{\%} refers to the amount of COOH groups of the polysaccharide involved in the cross-linking reaction, i.e. 50{\%} of the total amount. The rheological behaviour of the Hyal 50{\%} hydrogel, and in particular the possibility to inject it through a needle, was studied. The results obtained demonstrated that the hydrogel injected through the needle still behaved like a gel, although it showed a reduction of the dynamic moduli. The most appropriate sterilisation technique for this kind of hydrogel was also evaluated. Liophilised Hyal 50{\%} samples were sterilised by steam, Ethylene Oxide (EtO) and γ-rays. EtO and γ-rays did not modify the characteristics of the hydrogel in terms of swellability and morphology. Lastly, the in vivo effect of Hyal 50{\%} hydrogel in the treatment of chondral defect in rabbit knee was also studied. The results obtained showed the Hyal 50{\%} injections improved chondrocytes density and matrix appearance. Furthermore, the permanence in situ of the hydrogel was longer than that of the linear Hyal.",
keywords = "Hyaluronan hydrogel, In vivo tests, Osteoarthritis, Rheological properties, Sterilisation",
author = "R. Barbucci and S. Lamponi and A. Borzacchiello and L. Ambrosio and M. Fini and P. Torricelli and R. Giardino",
year = "2002",
month = "12",
doi = "10.1016/S0142-9612(02)00194-1",
language = "English",
volume = "23",
pages = "4503--4513",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier BV",
number = "23",

}

TY - JOUR

T1 - Hyaluronic acid hydrogel in the treatment of osteoarthritis

AU - Barbucci, R.

AU - Lamponi, S.

AU - Borzacchiello, A.

AU - Ambrosio, L.

AU - Fini, M.

AU - Torricelli, P.

AU - Giardino, R.

PY - 2002/12

Y1 - 2002/12

N2 - In order to overcome the problem of rapid clearance of the polysaccharide hyaluronic acid (Hyal) in the treatment of osteoarthritis (OA), a 50% cross-linked Hyal hydrogel (Hyal 50%) was synthesised. The 50% refers to the amount of COOH groups of the polysaccharide involved in the cross-linking reaction, i.e. 50% of the total amount. The rheological behaviour of the Hyal 50% hydrogel, and in particular the possibility to inject it through a needle, was studied. The results obtained demonstrated that the hydrogel injected through the needle still behaved like a gel, although it showed a reduction of the dynamic moduli. The most appropriate sterilisation technique for this kind of hydrogel was also evaluated. Liophilised Hyal 50% samples were sterilised by steam, Ethylene Oxide (EtO) and γ-rays. EtO and γ-rays did not modify the characteristics of the hydrogel in terms of swellability and morphology. Lastly, the in vivo effect of Hyal 50% hydrogel in the treatment of chondral defect in rabbit knee was also studied. The results obtained showed the Hyal 50% injections improved chondrocytes density and matrix appearance. Furthermore, the permanence in situ of the hydrogel was longer than that of the linear Hyal.

AB - In order to overcome the problem of rapid clearance of the polysaccharide hyaluronic acid (Hyal) in the treatment of osteoarthritis (OA), a 50% cross-linked Hyal hydrogel (Hyal 50%) was synthesised. The 50% refers to the amount of COOH groups of the polysaccharide involved in the cross-linking reaction, i.e. 50% of the total amount. The rheological behaviour of the Hyal 50% hydrogel, and in particular the possibility to inject it through a needle, was studied. The results obtained demonstrated that the hydrogel injected through the needle still behaved like a gel, although it showed a reduction of the dynamic moduli. The most appropriate sterilisation technique for this kind of hydrogel was also evaluated. Liophilised Hyal 50% samples were sterilised by steam, Ethylene Oxide (EtO) and γ-rays. EtO and γ-rays did not modify the characteristics of the hydrogel in terms of swellability and morphology. Lastly, the in vivo effect of Hyal 50% hydrogel in the treatment of chondral defect in rabbit knee was also studied. The results obtained showed the Hyal 50% injections improved chondrocytes density and matrix appearance. Furthermore, the permanence in situ of the hydrogel was longer than that of the linear Hyal.

KW - Hyaluronan hydrogel

KW - In vivo tests

KW - Osteoarthritis

KW - Rheological properties

KW - Sterilisation

UR - http://www.scopus.com/inward/record.url?scp=0036968183&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036968183&partnerID=8YFLogxK

U2 - 10.1016/S0142-9612(02)00194-1

DO - 10.1016/S0142-9612(02)00194-1

M3 - Article

C2 - 12322970

AN - SCOPUS:0036968183

VL - 23

SP - 4503

EP - 4513

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

IS - 23

ER -