Hyaluronidases for treating complications by hyaluronic acid dermal fillers: Evaluation of the effects on cell cultures and human skin

M. Cavallini, B. Antonioli, R. Gazzola, M. Tosca, M. Galuzzi, V. Rapisarda, F. Ciancio, M. Marazzi

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Hyaluronidases are commonly employed in aesthetic medicine to reverse the effects of the injected hyaluronic acid filler, eliminating nodules, correcting the injection of excessive quantities of filler, or reducing the odds of ischemic complications, although toxicity studies and clear guidelines are lacking in the literature. The objective of this study was to evaluate effects of hyaluronidases on skin and soft tissue viability. Methods: Human fibroblast cell and human skin cultures were grown under different concentrations of hyaluronidases. Cell viability and proliferation were tested with the hemocytometric assay with Trypan blue and colorimetric assay with 4,5-dimethylthiazol-22,5-difenil tetrazolium bromide. Results: The viability of fibroblast cultures was not significantly affected by the enzyme concentration or the time of exposure, exceeding 70 % of viability in all cases. The viability of the tissue samples showed a decrease, inferior to 70 % of viability at 6.5 and 14 U, independently from the time of exposure, although not statistically significant. Conclusions: Hyaluronidases do not affect fibroblast proliferation, although high concentrations of the enzyme may be able to inhibit human skin viability. This result could be influenced by the absence of growth medium; this result should be confirmed by further studies.

Original languageEnglish
Pages (from-to)477-484
Number of pages8
JournalEuropean Journal of Plastic Surgery
Volume36
Issue number8
DOIs
Publication statusPublished - Aug 2013

Keywords

  • Fibroblast
  • Filler
  • Hyaluronic acid
  • Hyaluronidase
  • Skin
  • Tissue
  • Toxicity

ASJC Scopus subject areas

  • Surgery

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