Hybrid composites made of multiwalled carbon nanotubes functionalized with Fe 3O 4 nanoparticles for tissue engineering applications

C. Cunha, S. Panseri, D. Iannazzo, A. Piperno, A. Pistone, M. Fazio, A. Russo, M. Marcacci, S. Galvagno

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

A straightforward technique for functionalization of multiwalled carbon nanotubes (MWCNTs) with magnetite (Fe 3O 4) nanoparticles was developed. Iron oxide nanoparticles were deposited on MWCNT surfaces by a depositionprecipitation method using Fe 3+/Fe 2+ salts precursors in basic solution. The characterizations by HRTEM, XRD, SEM/EDX, AAS and TPR analyses confirmed the successful formation of magnetic iron oxide nanoparticles on the MWCNT surface. Fe 3O 4/MWCNT hybrid composites were analysed in vitro by incubation with mesenchymal stem cells for 1, 3 and 7 days, either in the presence or absence of a static magnetic field. Analysis of cell proliferation was performed by the MTT assay, quantification of cellular stress was performed by the Lactate Dehydrogenase assay and analysis of cell morphology was performed by actin immunofluorescence and scanning electron microscopy. Results demonstrate that the introduction of magnetite into the MWCNT structure increases biocompatibility of oxidized MWCNTs. In addition, the presence of a static magnetic field further increases Fe 3O 4/MWCNT influence on cell behaviour. These results demonstrate this novel Fe 3O 4/MWCNT hybrid composite has good potential for tissue engineering applications.

Original languageEnglish
Article number465102
JournalNanotechnology
Volume23
Issue number46
DOIs
Publication statusPublished - Nov 23 2012

Fingerprint

Multiwalled carbon nanotubes (MWCN)
Tissue engineering
Nanoparticles
Composite materials
Ferrosoferric Oxide
Magnetite
Iron oxides
Assays
Magnetic fields
Scanning electron microscopy
Bioelectric potentials
Cell proliferation
Stem cells
Biocompatibility
L-Lactate Dehydrogenase
Actins
Energy dispersive spectroscopy
Salts

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Electrical and Electronic Engineering
  • Mechanical Engineering
  • Mechanics of Materials
  • Materials Science(all)

Cite this

Hybrid composites made of multiwalled carbon nanotubes functionalized with Fe 3O 4 nanoparticles for tissue engineering applications. / Cunha, C.; Panseri, S.; Iannazzo, D.; Piperno, A.; Pistone, A.; Fazio, M.; Russo, A.; Marcacci, M.; Galvagno, S.

In: Nanotechnology, Vol. 23, No. 46, 465102, 23.11.2012.

Research output: Contribution to journalArticle

Cunha, C. ; Panseri, S. ; Iannazzo, D. ; Piperno, A. ; Pistone, A. ; Fazio, M. ; Russo, A. ; Marcacci, M. ; Galvagno, S. / Hybrid composites made of multiwalled carbon nanotubes functionalized with Fe 3O 4 nanoparticles for tissue engineering applications. In: Nanotechnology. 2012 ; Vol. 23, No. 46.
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