Abstract
A straightforward technique for functionalization of multiwalled carbon nanotubes (MWCNTs) with magnetite (Fe 3O 4) nanoparticles was developed. Iron oxide nanoparticles were deposited on MWCNT surfaces by a depositionprecipitation method using Fe 3+/Fe 2+ salts precursors in basic solution. The characterizations by HRTEM, XRD, SEM/EDX, AAS and TPR analyses confirmed the successful formation of magnetic iron oxide nanoparticles on the MWCNT surface. Fe 3O 4/MWCNT hybrid composites were analysed in vitro by incubation with mesenchymal stem cells for 1, 3 and 7 days, either in the presence or absence of a static magnetic field. Analysis of cell proliferation was performed by the MTT assay, quantification of cellular stress was performed by the Lactate Dehydrogenase assay and analysis of cell morphology was performed by actin immunofluorescence and scanning electron microscopy. Results demonstrate that the introduction of magnetite into the MWCNT structure increases biocompatibility of oxidized MWCNTs. In addition, the presence of a static magnetic field further increases Fe 3O 4/MWCNT influence on cell behaviour. These results demonstrate this novel Fe 3O 4/MWCNT hybrid composite has good potential for tissue engineering applications.
| Original language | English |
|---|---|
| Article number | 465102 |
| Journal | Nanotechnology |
| Volume | 23 |
| Issue number | 46 |
| DOIs | |
| Publication status | Published - Nov 23 2012 |
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ASJC Scopus subject areas
- Bioengineering
- Chemistry(all)
- Electrical and Electronic Engineering
- Mechanical Engineering
- Mechanics of Materials
- Materials Science(all)
Cite this
Hybrid composites made of multiwalled carbon nanotubes functionalized with Fe 3O 4 nanoparticles for tissue engineering applications. / Cunha, C.; Panseri, S.; Iannazzo, D.; Piperno, A.; Pistone, A.; Fazio, M.; Russo, A.; Marcacci, M.; Galvagno, S.
In: Nanotechnology, Vol. 23, No. 46, 465102, 23.11.2012.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Hybrid composites made of multiwalled carbon nanotubes functionalized with Fe 3O 4 nanoparticles for tissue engineering applications
AU - Cunha, C.
AU - Panseri, S.
AU - Iannazzo, D.
AU - Piperno, A.
AU - Pistone, A.
AU - Fazio, M.
AU - Russo, A.
AU - Marcacci, M.
AU - Galvagno, S.
PY - 2012/11/23
Y1 - 2012/11/23
N2 - A straightforward technique for functionalization of multiwalled carbon nanotubes (MWCNTs) with magnetite (Fe 3O 4) nanoparticles was developed. Iron oxide nanoparticles were deposited on MWCNT surfaces by a depositionprecipitation method using Fe 3+/Fe 2+ salts precursors in basic solution. The characterizations by HRTEM, XRD, SEM/EDX, AAS and TPR analyses confirmed the successful formation of magnetic iron oxide nanoparticles on the MWCNT surface. Fe 3O 4/MWCNT hybrid composites were analysed in vitro by incubation with mesenchymal stem cells for 1, 3 and 7 days, either in the presence or absence of a static magnetic field. Analysis of cell proliferation was performed by the MTT assay, quantification of cellular stress was performed by the Lactate Dehydrogenase assay and analysis of cell morphology was performed by actin immunofluorescence and scanning electron microscopy. Results demonstrate that the introduction of magnetite into the MWCNT structure increases biocompatibility of oxidized MWCNTs. In addition, the presence of a static magnetic field further increases Fe 3O 4/MWCNT influence on cell behaviour. These results demonstrate this novel Fe 3O 4/MWCNT hybrid composite has good potential for tissue engineering applications.
AB - A straightforward technique for functionalization of multiwalled carbon nanotubes (MWCNTs) with magnetite (Fe 3O 4) nanoparticles was developed. Iron oxide nanoparticles were deposited on MWCNT surfaces by a depositionprecipitation method using Fe 3+/Fe 2+ salts precursors in basic solution. The characterizations by HRTEM, XRD, SEM/EDX, AAS and TPR analyses confirmed the successful formation of magnetic iron oxide nanoparticles on the MWCNT surface. Fe 3O 4/MWCNT hybrid composites were analysed in vitro by incubation with mesenchymal stem cells for 1, 3 and 7 days, either in the presence or absence of a static magnetic field. Analysis of cell proliferation was performed by the MTT assay, quantification of cellular stress was performed by the Lactate Dehydrogenase assay and analysis of cell morphology was performed by actin immunofluorescence and scanning electron microscopy. Results demonstrate that the introduction of magnetite into the MWCNT structure increases biocompatibility of oxidized MWCNTs. In addition, the presence of a static magnetic field further increases Fe 3O 4/MWCNT influence on cell behaviour. These results demonstrate this novel Fe 3O 4/MWCNT hybrid composite has good potential for tissue engineering applications.
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U2 - 10.1088/0957-4484/23/46/465102
DO - 10.1088/0957-4484/23/46/465102
M3 - Article
VL - 23
JO - Nanotechnology
JF - Nanotechnology
SN - 0957-4484
IS - 46
M1 - 465102
ER -