Hybrid Rop-pIII proteins for the display of constrained peptides on filamentous phage capsids

N. Santiago Vispo, F. Felici, L. Castagnoli, G. Cesareni

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

In order to increase the versatility of phage display technology, it is desirable to be able to impose some structural constraints on the peptides that are presented by the phage particles. This is currently not feasible since the conformation of the capsid proteins, used to link the foreign peptide to the phage, are either unknown (pIII) or too simple (pVIII) to permit the engineering of peptide inserts into a constrained context. To reach this scope we have modified the amino-terminus of gene III by appending a well-characterized protein motif, the four-helix bundle of the bacterial protein Rop. Phage particles displaying Rop can be separated from wild-type (wt) particles by affinity purification with an antibody. Rop can be extensively modified by substituting its solvent-exposed residues and/or by inserting peptides either into the carboxy-terminal tail or into the bend region that connects the two α-helices of the monomer. These results open the possibility to construct peptide libraries where the peptides are constrained either into an Ω-loop type conformation or an α-helix. Libraries formed by peptides inserted into the carboxy-terminus can also be constructed. Furthermore, the system that we have developed permits to produce large quantities of the elements of the libraries in the cytoplasm or to display them on the capsid of filamentous phages.

Original languageEnglish
Pages (from-to)917-922
Number of pages6
JournalAnnales de Biologie Clinique
Volume51
Issue number10-11
Publication statusPublished - 1993

Fingerprint

Inovirus
Bacteriophages
capsid
Capsid
Display devices
peptides
bacteriophages
Peptides
Peptide Library
Proteins
proteins
Conformations
Amino Acid Motifs
Bacterial Proteins
peptide libraries
Capsid Proteins
bacterial proteins
Libraries
coat proteins
Cytoplasm

Keywords

  • peptides
  • phage

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Medicine (miscellaneous)

Cite this

Hybrid Rop-pIII proteins for the display of constrained peptides on filamentous phage capsids. / Santiago Vispo, N.; Felici, F.; Castagnoli, L.; Cesareni, G.

In: Annales de Biologie Clinique, Vol. 51, No. 10-11, 1993, p. 917-922.

Research output: Contribution to journalArticle

Santiago Vispo, N. ; Felici, F. ; Castagnoli, L. ; Cesareni, G. / Hybrid Rop-pIII proteins for the display of constrained peptides on filamentous phage capsids. In: Annales de Biologie Clinique. 1993 ; Vol. 51, No. 10-11. pp. 917-922.
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