Hydrogen sulfide-releasing silk fibroin scaffold for bone tissue engineering

Laura Gambari, Emanuela Amore, Rosasilvia Raggio, Walter Bonani, Marli Barone, Gina Lisignoli, Brunella Grigolo, Antonella Motta, Francesco Grassi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Hydrogen sulfide (H 2 S)-based therapy is a promising therapeutic strategy for several biomedical applications. Following the observation that endogenous and exogenous H 2 S plays a prominent role as a bone anabolic agent, we recently developed a silk fibroin (SF) porous scaffold loaded with GYY4137 (GYY), an H 2 S donor, for applications in bone tissue engineering. Here, we assayed whether the combination of SF with H 2 S potentiates the osteoconductive properties of SF. Biocompatibility and osteoanabolic activity were assayed in vitro using human bone marrow mesenchymal stromal cells. Cell cultures were performed on a perfusion bioreactor to obtain results closer to the in vivo microenvironment. Cytotoxicity was excluded by lactate dehydrogenase and live/dead assays. Cell colonization and mineral apposition were evaluated by Haematoxylin & Eosin and Von Kossa/Alizarin Red-S stainings respectively. PCR array for human osteogenesis and immunohistochemical analyses were performed to identify pathways and targets involved. Our findings show that H 2 S-releasing SF scaffolds supported cell adhesion, proliferation and viability. Moreover, H 2 S activated genes and proteins involved in ossification, osteoblast differentiation, bone mineral metabolism and angiogenesis allowing a high and early mineralization. Based on these properties, we suggest the use of H 2 S-releasing SF scaffolds for bone healing and regeneration applications.

Original languageEnglish
Pages (from-to)471-482
Number of pages12
JournalMaterials Science and Engineering C
Volume102
DOIs
Publication statusPublished - Sep 1 2019

Fingerprint

Fibroins
silk
Hydrogen Sulfide
Silk
hydrogen sulfide
tissue engineering
Hydrogen sulfide
releasing
Scaffolds (biology)
Tissue engineering
bones
Bone
Scaffolds
mineral metabolism
Minerals
Alizarin
osteogenesis
bioreactors
Anabolic Agents
angiogenesis

Keywords

  • Bone tissue engineering
  • Hydrogen sulfide
  • Mesenchymal stromal cells
  • Osteogenesis
  • Scaffold
  • Silk fibroin

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

Cite this

Hydrogen sulfide-releasing silk fibroin scaffold for bone tissue engineering. / Gambari, Laura; Amore, Emanuela; Raggio, Rosasilvia; Bonani, Walter; Barone, Marli; Lisignoli, Gina; Grigolo, Brunella; Motta, Antonella; Grassi, Francesco.

In: Materials Science and Engineering C, Vol. 102, 01.09.2019, p. 471-482.

Research output: Contribution to journalArticle

Gambari, Laura ; Amore, Emanuela ; Raggio, Rosasilvia ; Bonani, Walter ; Barone, Marli ; Lisignoli, Gina ; Grigolo, Brunella ; Motta, Antonella ; Grassi, Francesco. / Hydrogen sulfide-releasing silk fibroin scaffold for bone tissue engineering. In: Materials Science and Engineering C. 2019 ; Vol. 102. pp. 471-482.
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