Abstract
Hydrogen sulfide (H 2 S)-based therapy is a promising therapeutic strategy for several biomedical applications. Following the observation that endogenous and exogenous H 2 S plays a prominent role as a bone anabolic agent, we recently developed a silk fibroin (SF) porous scaffold loaded with GYY4137 (GYY), an H 2 S donor, for applications in bone tissue engineering. Here, we assayed whether the combination of SF with H 2 S potentiates the osteoconductive properties of SF. Biocompatibility and osteoanabolic activity were assayed in vitro using human bone marrow mesenchymal stromal cells. Cell cultures were performed on a perfusion bioreactor to obtain results closer to the in vivo microenvironment. Cytotoxicity was excluded by lactate dehydrogenase and live/dead assays. Cell colonization and mineral apposition were evaluated by Haematoxylin & Eosin and Von Kossa/Alizarin Red-S stainings respectively. PCR array for human osteogenesis and immunohistochemical analyses were performed to identify pathways and targets involved. Our findings show that H 2 S-releasing SF scaffolds supported cell adhesion, proliferation and viability. Moreover, H 2 S activated genes and proteins involved in ossification, osteoblast differentiation, bone mineral metabolism and angiogenesis allowing a high and early mineralization. Based on these properties, we suggest the use of H 2 S-releasing SF scaffolds for bone healing and regeneration applications.
| Original language | English |
|---|---|
| Pages (from-to) | 471-482 |
| Number of pages | 12 |
| Journal | Materials Science and Engineering C |
| Volume | 102 |
| DOIs | |
| Publication status | Published - Sep 1 2019 |
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Keywords
- Bone tissue engineering
- Hydrogen sulfide
- Mesenchymal stromal cells
- Osteogenesis
- Scaffold
- Silk fibroin
ASJC Scopus subject areas
- Materials Science(all)
- Condensed Matter Physics
- Mechanics of Materials
- Mechanical Engineering
Cite this
Hydrogen sulfide-releasing silk fibroin scaffold for bone tissue engineering. / Gambari, Laura; Amore, Emanuela; Raggio, Rosasilvia; Bonani, Walter; Barone, Marli; Lisignoli, Gina; Grigolo, Brunella; Motta, Antonella; Grassi, Francesco.
In: Materials Science and Engineering C, Vol. 102, 01.09.2019, p. 471-482.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Hydrogen sulfide-releasing silk fibroin scaffold for bone tissue engineering
AU - Gambari, Laura
AU - Amore, Emanuela
AU - Raggio, Rosasilvia
AU - Bonani, Walter
AU - Barone, Marli
AU - Lisignoli, Gina
AU - Grigolo, Brunella
AU - Motta, Antonella
AU - Grassi, Francesco
PY - 2019/9/1
Y1 - 2019/9/1
N2 - Hydrogen sulfide (H 2 S)-based therapy is a promising therapeutic strategy for several biomedical applications. Following the observation that endogenous and exogenous H 2 S plays a prominent role as a bone anabolic agent, we recently developed a silk fibroin (SF) porous scaffold loaded with GYY4137 (GYY), an H 2 S donor, for applications in bone tissue engineering. Here, we assayed whether the combination of SF with H 2 S potentiates the osteoconductive properties of SF. Biocompatibility and osteoanabolic activity were assayed in vitro using human bone marrow mesenchymal stromal cells. Cell cultures were performed on a perfusion bioreactor to obtain results closer to the in vivo microenvironment. Cytotoxicity was excluded by lactate dehydrogenase and live/dead assays. Cell colonization and mineral apposition were evaluated by Haematoxylin & Eosin and Von Kossa/Alizarin Red-S stainings respectively. PCR array for human osteogenesis and immunohistochemical analyses were performed to identify pathways and targets involved. Our findings show that H 2 S-releasing SF scaffolds supported cell adhesion, proliferation and viability. Moreover, H 2 S activated genes and proteins involved in ossification, osteoblast differentiation, bone mineral metabolism and angiogenesis allowing a high and early mineralization. Based on these properties, we suggest the use of H 2 S-releasing SF scaffolds for bone healing and regeneration applications.
AB - Hydrogen sulfide (H 2 S)-based therapy is a promising therapeutic strategy for several biomedical applications. Following the observation that endogenous and exogenous H 2 S plays a prominent role as a bone anabolic agent, we recently developed a silk fibroin (SF) porous scaffold loaded with GYY4137 (GYY), an H 2 S donor, for applications in bone tissue engineering. Here, we assayed whether the combination of SF with H 2 S potentiates the osteoconductive properties of SF. Biocompatibility and osteoanabolic activity were assayed in vitro using human bone marrow mesenchymal stromal cells. Cell cultures were performed on a perfusion bioreactor to obtain results closer to the in vivo microenvironment. Cytotoxicity was excluded by lactate dehydrogenase and live/dead assays. Cell colonization and mineral apposition were evaluated by Haematoxylin & Eosin and Von Kossa/Alizarin Red-S stainings respectively. PCR array for human osteogenesis and immunohistochemical analyses were performed to identify pathways and targets involved. Our findings show that H 2 S-releasing SF scaffolds supported cell adhesion, proliferation and viability. Moreover, H 2 S activated genes and proteins involved in ossification, osteoblast differentiation, bone mineral metabolism and angiogenesis allowing a high and early mineralization. Based on these properties, we suggest the use of H 2 S-releasing SF scaffolds for bone healing and regeneration applications.
KW - Bone tissue engineering
KW - Hydrogen sulfide
KW - Mesenchymal stromal cells
KW - Osteogenesis
KW - Scaffold
KW - Silk fibroin
UR - http://www.scopus.com/inward/record.url?scp=85064835908&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85064835908&partnerID=8YFLogxK
U2 - 10.1016/j.msec.2019.04.039
DO - 10.1016/j.msec.2019.04.039
M3 - Article
AN - SCOPUS:85064835908
VL - 102
SP - 471
EP - 482
JO - Materials Science and Engineering C
JF - Materials Science and Engineering C
SN - 0928-4931
ER -