Hydroxyl radical generation, levels of tumor necrosis factor-alpha, and progression to heart failure after acute myocardial infarction

Marco Valgimigli, Elisa Merli, Patrizia Malagutti, Olga Soukhomovskaia, Giordano Cicchitelli, Alessandra Antelli, Donatella Canistro, Gloria Francolini, Gaetano Macrì, Francesca Mastrorilli, Moreno Paolini, Roberto Ferrari

Research output: Contribution to journalArticlepeer-review


Objectives We used acetylsalicylic acid (ASA) as a probing agent to quantify hydroxyl radical (·OH) in Controls and patients with coronary artery disease and to prospectively investigate ·OH production in patients with myocardial infarction (MI) complicated by heart failure (HF). Background Oxidative stress status (OSS) is a mechanism for transition to HF in experimental heart injury models, but evidence for its causal role in humans is still limited. Methods Thirty healthy subjects (Controls), 12 patients with stable angina (Group 1), and 74 patients with ST-segment elevation MI (Group 2) were enrolled. A dose of 250 mg Flectadol was given intravenously before each blood collection to determine the 2,3-dihydroxybenzoic acid/salicylic acid (DHBA/SA) ratio. We also quantified vitamin E and coenzyme Q10 to monitor antioxidant reserve, as well as tumor necrosis factor (TNF)-alpha, TNF-soluble receptors, interleukin (IL)-6, and IL-1ra to assess inflammatory status. All measurements were repeated at month 6 in Group 2. Results There were no differences between Controls and Group 1. Group 2 showed increased ·OH production, peaking at 24 h, whereas vitamin E and coenzyme Q10 progressively declined. Group 2 patients developing HF during hospitalization (Group 2Bi) presented with an increase of both ·OH production at discharge and inflammatory status, as compared with patients without HF (Group 2Ai), persisting at month 6 in post-MI patients with HF (Group 2Bii). Conclusions We found a distinct pattern of ·OH generation in post-MI patients who show progression to HF. The interplay between OSS and inflammatory status should be targeted as a possible mechanism of progression to post-MI left ventricular dysfunction.

Original languageEnglish
Pages (from-to)2000-2008
Number of pages9
JournalJournal of the American College of Cardiology
Issue number11
Publication statusPublished - Jun 2 2004


  • OH
  • acetylsalicylic acid
  • ASA
  • DHBA
  • dihydroxybenzoic acid
  • heart failure
  • HF
  • hydroxyl radical
  • IL
  • interleukin
  • left ventricular
  • LV
  • MI
  • myocardial infarction
  • OSS
  • oxidative stress status
  • reactive oxygen species
  • ROS
  • SA
  • salicylic acid
  • sTNFR

ASJC Scopus subject areas

  • Nursing(all)


Dive into the research topics of 'Hydroxyl radical generation, levels of tumor necrosis factor-alpha, and progression to heart failure after acute myocardial infarction'. Together they form a unique fingerprint.

Cite this