Hyperglycemia-induced thrombin formation in diabetes

The possible role of oxidative stress

A. Ceriello, R. Giacomello, G. Stel, E. Motz, C. Taboga, L. Tonutti, M. Pirisi, E. Falleti, E. Bartoli

Research output: Contribution to journalArticle

180 Citations (Scopus)

Abstract

Diabetes is characterized by the existence of a thrombosis-prone condition, possibly related to hyperglycemia. However, the mechanism linking hyperglycemia to the activation of the coagulation cascade is still unclear. It has been recently suggested that diabetes is accompanied by increased oxidative stress. In this work, the possibility that oxidative stress may be involved in the hyperglycemia-induced coagulation activation has been evaluated. Prothrombin fragment 1 + 2 (F1+2), which represents a reliable marker of the amount of thrombin released in the circulation, has been chosen for studying thrombin formation in vivo. In nine type II diabetic patients and in seven healthy control subjects, matched for age and body mass index, three different experiments were performed: oral glucose tolerance test (OGTT), intravenous antioxidant glutathione (GSH) administration for 2 h, and OGTT plus intravenous GSH administration. Samples were drawn at -15 min and every 30 min from 0 to 180 min. During the OGTT, F1+2 significantly increased in both diabetic and healthy subjects. GSH administration during OGTT normalized this phenomenon. GSH administered alone significantly decreased F1+2 in diabetic patients, while no effect was observed in the normal subjects. These data suggest that hyperglycemia may induce thrombin activation, possibly inducing an oxidative stress, and that antioxidant GSH may counterbalance this effect.

Original languageEnglish
Pages (from-to)924-928
Number of pages5
JournalDiabetes
Volume44
Issue number8
Publication statusPublished - 1995

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Glucose Tolerance Test
Thrombin
Hyperglycemia
Oxidative Stress
Healthy Volunteers
Antioxidants
Intravenous Administration
Glutathione
Body Mass Index
Thrombosis

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Internal Medicine

Cite this

Ceriello, A., Giacomello, R., Stel, G., Motz, E., Taboga, C., Tonutti, L., ... Bartoli, E. (1995). Hyperglycemia-induced thrombin formation in diabetes: The possible role of oxidative stress. Diabetes, 44(8), 924-928.

Hyperglycemia-induced thrombin formation in diabetes : The possible role of oxidative stress. / Ceriello, A.; Giacomello, R.; Stel, G.; Motz, E.; Taboga, C.; Tonutti, L.; Pirisi, M.; Falleti, E.; Bartoli, E.

In: Diabetes, Vol. 44, No. 8, 1995, p. 924-928.

Research output: Contribution to journalArticle

Ceriello, A, Giacomello, R, Stel, G, Motz, E, Taboga, C, Tonutti, L, Pirisi, M, Falleti, E & Bartoli, E 1995, 'Hyperglycemia-induced thrombin formation in diabetes: The possible role of oxidative stress', Diabetes, vol. 44, no. 8, pp. 924-928.
Ceriello A, Giacomello R, Stel G, Motz E, Taboga C, Tonutti L et al. Hyperglycemia-induced thrombin formation in diabetes: The possible role of oxidative stress. Diabetes. 1995;44(8):924-928.
Ceriello, A. ; Giacomello, R. ; Stel, G. ; Motz, E. ; Taboga, C. ; Tonutti, L. ; Pirisi, M. ; Falleti, E. ; Bartoli, E. / Hyperglycemia-induced thrombin formation in diabetes : The possible role of oxidative stress. In: Diabetes. 1995 ; Vol. 44, No. 8. pp. 924-928.
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