Abstract
A variety of evidence suggest that cardiovascular system functions are impaired in altered gravity conditions [1]. In this study we investigated the influence of hypergravity environment (3g) on endothelial cell proliferation, endothelial vasoactive compound production and on in vitro angiogenesis. We found that cultured primary human endothial cells were very sensitive to mild hypergravity conditions. Even if we did not record changes in cell viability and apoptosis, we found significant differences in cell proliferation, prostacyclin (PGI2) synthesis, nitric oxide (NO) synthesis, and in angiogenic responses. Using western blotting technique we detected an increased expression of cycloxygenase-2 (COX-2) in primary endothelial cells exposed for 48 hours to hypergravity, in comparison to those exposed to normal gravity.
| Original language | English |
|---|---|
| Title of host publication | European Space Agency, (Special Publication) ESA SP |
| Pages | 327-328 |
| Number of pages | 2 |
| Edition | 501 |
| Publication status | Published - Sep 2002 |
| Event | Proceedings of the European Symposium on Life in Space for Life on Earth - Stockholm, Sweden Duration: Jun 2 2002 → Jun 7 2002 |
Other
| Other | Proceedings of the European Symposium on Life in Space for Life on Earth |
|---|---|
| Country | Sweden |
| City | Stockholm |
| Period | 6/2/02 → 6/7/02 |
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ASJC Scopus subject areas
- Aerospace Engineering
Cite this
Hypergravity impairs angiogenic response of in vitro cultured human primary endothelial cells. / Spisni, Enzo; Bianco, Maria Cristina; Blasi, Francesca; Santi, Spartaco; Toni, Mattia; Griffoni, Cristiana; Tomasi, Vittorio.
European Space Agency, (Special Publication) ESA SP. 501. ed. 2002. p. 327-328.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Hypergravity impairs angiogenic response of in vitro cultured human primary endothelial cells
AU - Spisni, Enzo
AU - Bianco, Maria Cristina
AU - Blasi, Francesca
AU - Santi, Spartaco
AU - Toni, Mattia
AU - Griffoni, Cristiana
AU - Tomasi, Vittorio
PY - 2002/9
Y1 - 2002/9
N2 - A variety of evidence suggest that cardiovascular system functions are impaired in altered gravity conditions [1]. In this study we investigated the influence of hypergravity environment (3g) on endothelial cell proliferation, endothelial vasoactive compound production and on in vitro angiogenesis. We found that cultured primary human endothial cells were very sensitive to mild hypergravity conditions. Even if we did not record changes in cell viability and apoptosis, we found significant differences in cell proliferation, prostacyclin (PGI2) synthesis, nitric oxide (NO) synthesis, and in angiogenic responses. Using western blotting technique we detected an increased expression of cycloxygenase-2 (COX-2) in primary endothelial cells exposed for 48 hours to hypergravity, in comparison to those exposed to normal gravity.
AB - A variety of evidence suggest that cardiovascular system functions are impaired in altered gravity conditions [1]. In this study we investigated the influence of hypergravity environment (3g) on endothelial cell proliferation, endothelial vasoactive compound production and on in vitro angiogenesis. We found that cultured primary human endothial cells were very sensitive to mild hypergravity conditions. Even if we did not record changes in cell viability and apoptosis, we found significant differences in cell proliferation, prostacyclin (PGI2) synthesis, nitric oxide (NO) synthesis, and in angiogenic responses. Using western blotting technique we detected an increased expression of cycloxygenase-2 (COX-2) in primary endothelial cells exposed for 48 hours to hypergravity, in comparison to those exposed to normal gravity.
UR - http://www.scopus.com/inward/record.url?scp=0036770976&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036770976&partnerID=8YFLogxK
M3 - Conference contribution
SP - 327
EP - 328
BT - European Space Agency, (Special Publication) ESA SP
ER -