Hypergravity impairs angiogenic response of in vitro cultured human primary endothelial cells

Enzo Spisni; Maria Cristina Bianco; Francesca Blasi; Spartaco Santi; Mattia Toni; Cristiana Griffoni; Vittorio Tomasi

Hypergravity impairs angiogenic response of in vitro cultured human primary endothelial cells

Enzo Spisni, Maria Cristina Bianco, Francesca Blasi, Spartaco Santi, Mattia Toni, Cristiana Griffoni, Vittorio Tomasi

Istituti Ortopedici Rizzoli

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

A variety of evidence suggest that cardiovascular system functions are impaired in altered gravity conditions [1]. In this study we investigated the influence of hypergravity environment (3g) on endothelial cell proliferation, endothelial vasoactive compound production and on in vitro angiogenesis. We found that cultured primary human endothial cells were very sensitive to mild hypergravity conditions. Even if we did not record changes in cell viability and apoptosis, we found significant differences in cell proliferation, prostacyclin (PGI2) synthesis, nitric oxide (NO) synthesis, and in angiogenic responses. Using western blotting technique we detected an increased expression of cycloxygenase-2 (COX-2) in primary endothelial cells exposed for 48 hours to hypergravity, in comparison to those exposed to normal gravity.

Original language	English
Title of host publication	European Space Agency, (Special Publication) ESA SP
Pages	327-328
Number of pages	2
Edition	501
Publication status	Published - Sep 2002
Event	Proceedings of the European Symposium on Life in Space for Life on Earth - Stockholm, Sweden Duration: Jun 2 2002 → Jun 7 2002

Other

Other	Proceedings of the European Symposium on Life in Space for Life on Earth
Country	Sweden
City	Stockholm
Period	6/2/02 → 6/7/02

ASJC Scopus subject areas

Aerospace Engineering

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Hypergravity impairs angiogenic response of in vitro cultured human primary endothelial cells. / Spisni, Enzo; Bianco, Maria Cristina; Blasi, Francesca; Santi, Spartaco; Toni, Mattia; Griffoni, Cristiana; Tomasi, Vittorio.

European Space Agency, (Special Publication) ESA SP. 501. ed. 2002. p. 327-328.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

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abstract = "A variety of evidence suggest that cardiovascular system functions are impaired in altered gravity conditions [1]. In this study we investigated the influence of hypergravity environment (3g) on endothelial cell proliferation, endothelial vasoactive compound production and on in vitro angiogenesis. We found that cultured primary human endothial cells were very sensitive to mild hypergravity conditions. Even if we did not record changes in cell viability and apoptosis, we found significant differences in cell proliferation, prostacyclin (PGI2) synthesis, nitric oxide (NO) synthesis, and in angiogenic responses. Using western blotting technique we detected an increased expression of cycloxygenase-2 (COX-2) in primary endothelial cells exposed for 48 hours to hypergravity, in comparison to those exposed to normal gravity.",

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AU - Spisni, Enzo

AU - Bianco, Maria Cristina

AU - Blasi, Francesca

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AU - Toni, Mattia

AU - Griffoni, Cristiana

AU - Tomasi, Vittorio

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AB - A variety of evidence suggest that cardiovascular system functions are impaired in altered gravity conditions [1]. In this study we investigated the influence of hypergravity environment (3g) on endothelial cell proliferation, endothelial vasoactive compound production and on in vitro angiogenesis. We found that cultured primary human endothial cells were very sensitive to mild hypergravity conditions. Even if we did not record changes in cell viability and apoptosis, we found significant differences in cell proliferation, prostacyclin (PGI2) synthesis, nitric oxide (NO) synthesis, and in angiogenic responses. Using western blotting technique we detected an increased expression of cycloxygenase-2 (COX-2) in primary endothelial cells exposed for 48 hours to hypergravity, in comparison to those exposed to normal gravity.

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