Hypertension-associated point mutations in the adducin α and β subunits affect actin cytoskeleton and ion transport

Grazia Tripodi, Flavia Valtorta, Lucia Torielli, Evelina Chieregatti, Sergio Salardi, Livio Trusolino, Andrea Menegon, Patrizia Ferrari, Pier Carlo Marchisio, Giuseppe Bianchi

Research output: Contribution to journalArticle

Abstract

The adducin heterodimer is a protein affecting the assembly of the actin- based cytoskeleton. Point mutations in rat adducin α (F316Y) and β (Q529R) subunits are involved in a form of rat primary hypertension (MHS) associated with faster kidney tubular ion transport. A role for adducin in human primary hypertension has also been suggested. By studying the interaction of actin with purified normal and mutated adducin in a cell-free system and the actin assembly in rat kidney epithelial cells (NRK-52E) transfected with mutated rat adducin cDNA, we show that the adducin isoforms differentially modulate: (a) actin assembly both in a cell-free system and within transfected cells; (b) topography of αV integrin together with focal contact proteins; and (c) Na-K pump activity at V(max) (faster with the mutated isoforms, 1281±90 vs 841±30 nmol K/h · mg pt., P <0.0001). This co-modulation suggests a role for adducin in the constitutive capacity of the epithelia both to transport ions and to expose adhesion molecules. These findings may also lead to the understanding of the relation between adducin polymorphism and blood pressure and to the development of new approaches to the study of hypertension- associated organ damage.

Original languageEnglish
Pages (from-to)2815-2822
Number of pages8
JournalJournal of Clinical Investigation
Volume97
Issue number12
Publication statusPublished - Jun 15 1996

Keywords

  • adhesion molecules
  • blood pressure
  • genetics
  • Na(+)-K(+)ATPase
  • rat

ASJC Scopus subject areas

  • Medicine(all)

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    Tripodi, G., Valtorta, F., Torielli, L., Chieregatti, E., Salardi, S., Trusolino, L., Menegon, A., Ferrari, P., Marchisio, P. C., & Bianchi, G. (1996). Hypertension-associated point mutations in the adducin α and β subunits affect actin cytoskeleton and ion transport. Journal of Clinical Investigation, 97(12), 2815-2822.