TY - JOUR
T1 - Hypertensive congenital adrenal enzymatic defects detected by high-performance liquid chromatography of corticosteroids
AU - Carpenè, G.
AU - Vettoretti, A.
AU - Pedini, F.
AU - Rocco, S.
AU - Mantero, F.
AU - Opocher, G.
PY - 1991/8/16
Y1 - 1991/8/16
N2 - The simultaneous measurement of the adrenal deoxycorticosterone (DOC), 18-OH-DOC, corticosterone (B), 18-OH-B, 11 -deoxycortisol (S) and cortisol (F) present in human plasma in cases of adrenal dysfunction was accomplished using a high-performance liquid chromatographic (HPLC) system with a UV detector and with a radioimmunoassay (RIA). After a solid-phase extraction, plasma samples were separated by HPLC using a gradient of water-acetonitrile-ethanol on a radial compressed reversed-phase column. In a 70-min cycle, a complete separation of adrenal steroids was accomplished. The UV detector allowed direct measurement of F in each plasma sample while in selected cases B and S were directly determined. It was therefore possible quickly to identify patients with hypertensive congenital adrenal enzymatic defects with this method: the 17-α-hydroxylase deficiency characterized by the absence of measurable levels of F with an evident peak corresponding to B and the 11-β-hydroxylase deficiency in which high levels of S without F are detected. The RIA of DOC, B, 18-OH-DOC and 18-OH-B complete the characterization of the adrenal defect. Therefore, with this HPLC method it is possible to recognize the major hypertensive adrenal enzymatic deficiencies such as the defect of 17-α-hydroxylase or 11 -β-hydroxylase. With "RIA" detectors an almost complete spectrum of adrenal steroid secretion can be obtained.
AB - The simultaneous measurement of the adrenal deoxycorticosterone (DOC), 18-OH-DOC, corticosterone (B), 18-OH-B, 11 -deoxycortisol (S) and cortisol (F) present in human plasma in cases of adrenal dysfunction was accomplished using a high-performance liquid chromatographic (HPLC) system with a UV detector and with a radioimmunoassay (RIA). After a solid-phase extraction, plasma samples were separated by HPLC using a gradient of water-acetonitrile-ethanol on a radial compressed reversed-phase column. In a 70-min cycle, a complete separation of adrenal steroids was accomplished. The UV detector allowed direct measurement of F in each plasma sample while in selected cases B and S were directly determined. It was therefore possible quickly to identify patients with hypertensive congenital adrenal enzymatic defects with this method: the 17-α-hydroxylase deficiency characterized by the absence of measurable levels of F with an evident peak corresponding to B and the 11-β-hydroxylase deficiency in which high levels of S without F are detected. The RIA of DOC, B, 18-OH-DOC and 18-OH-B complete the characterization of the adrenal defect. Therefore, with this HPLC method it is possible to recognize the major hypertensive adrenal enzymatic deficiencies such as the defect of 17-α-hydroxylase or 11 -β-hydroxylase. With "RIA" detectors an almost complete spectrum of adrenal steroid secretion can be obtained.
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U2 - 10.1016/S0021-9673(01)88489-2
DO - 10.1016/S0021-9673(01)88489-2
M3 - Article
C2 - 1787153
AN - SCOPUS:0025873528
VL - 553
SP - 201
EP - 204
JO - Journal of Chromatography A
JF - Journal of Chromatography A
SN - 0021-9673
IS - C
ER -