Hyposialylated α1-acid glycoprotein inhibits phagocytosis of feline neutrophils

G. Rossi, L. Capitani, F. Ceciliani, L. Restelli, S. Paltrinieri

Research output: Contribution to journalArticlepeer-review

Abstract

Feline α1-acid glycoprotein (fAGP) modifies both its serum concentration and its glycan moiety during diseases. fAGP is hyposialylated in cats with feline infectious peritonitis (FIP), but not in clinically healthy cats or in cats with other diseases. This study was aimed to determine whether hyposialylated fAGP influences phagocytosis. A flow cytometric method based on ingestion of fluoresceinated bacteria and adapted to feline blood was used to assess phagocytosis of leukocytes incubated with 'non-pathological' fAGP (purified from sera with normal concentrations of AGP) and 'pathological' fAGP (purified from sera with >1.5. mg/mL hyposialylated AGP). The flow cytometric method provided repeatable results for neutrophils (coefficients of variations, CVs 20%) which had also a high individual variability. Compared with saline solution and with non-pathological fAGP, pathological fAGP significantly decreased phagocytosis in neutrophils and monocytes. This study demonstrated that hyposialylated fAGP down-regulates the phagocytic activity of feline neutrophils.

Original languageEnglish
Pages (from-to)465-471
Number of pages7
JournalResearch in Veterinary Science
Volume95
Issue number2
DOIs
Publication statusPublished - Oct 2013

Keywords

  • α-1-Acid glycoprotein
  • Feline
  • Feline infectious peritonitis
  • Flow cytometry
  • Phagocytosis

ASJC Scopus subject areas

  • veterinary(all)

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