TY - JOUR
T1 - Hyposialylated α1-acid glycoprotein inhibits phagocytosis of feline neutrophils
AU - Rossi, G.
AU - Capitani, L.
AU - Ceciliani, F.
AU - Restelli, L.
AU - Paltrinieri, S.
PY - 2013/10
Y1 - 2013/10
N2 - Feline α1-acid glycoprotein (fAGP) modifies both its serum concentration and its glycan moiety during diseases. fAGP is hyposialylated in cats with feline infectious peritonitis (FIP), but not in clinically healthy cats or in cats with other diseases. This study was aimed to determine whether hyposialylated fAGP influences phagocytosis. A flow cytometric method based on ingestion of fluoresceinated bacteria and adapted to feline blood was used to assess phagocytosis of leukocytes incubated with 'non-pathological' fAGP (purified from sera with normal concentrations of AGP) and 'pathological' fAGP (purified from sera with >1.5. mg/mL hyposialylated AGP). The flow cytometric method provided repeatable results for neutrophils (coefficients of variations, CVs 20%) which had also a high individual variability. Compared with saline solution and with non-pathological fAGP, pathological fAGP significantly decreased phagocytosis in neutrophils and monocytes. This study demonstrated that hyposialylated fAGP down-regulates the phagocytic activity of feline neutrophils.
AB - Feline α1-acid glycoprotein (fAGP) modifies both its serum concentration and its glycan moiety during diseases. fAGP is hyposialylated in cats with feline infectious peritonitis (FIP), but not in clinically healthy cats or in cats with other diseases. This study was aimed to determine whether hyposialylated fAGP influences phagocytosis. A flow cytometric method based on ingestion of fluoresceinated bacteria and adapted to feline blood was used to assess phagocytosis of leukocytes incubated with 'non-pathological' fAGP (purified from sera with normal concentrations of AGP) and 'pathological' fAGP (purified from sera with >1.5. mg/mL hyposialylated AGP). The flow cytometric method provided repeatable results for neutrophils (coefficients of variations, CVs 20%) which had also a high individual variability. Compared with saline solution and with non-pathological fAGP, pathological fAGP significantly decreased phagocytosis in neutrophils and monocytes. This study demonstrated that hyposialylated fAGP down-regulates the phagocytic activity of feline neutrophils.
KW - α-1-Acid glycoprotein
KW - Feline
KW - Feline infectious peritonitis
KW - Flow cytometry
KW - Phagocytosis
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UR - http://www.scopus.com/inward/citedby.url?scp=84881552045&partnerID=8YFLogxK
U2 - 10.1016/j.rvsc.2013.04.026
DO - 10.1016/j.rvsc.2013.04.026
M3 - Article
C2 - 23726663
AN - SCOPUS:84881552045
VL - 95
SP - 465
EP - 471
JO - Research in Veterinary Science
JF - Research in Veterinary Science
SN - 0034-5288
IS - 2
ER -