Abstract
Aim: Recent investigations have reported improved myocardial function during hypothermia following resuscitation from cardiac arrest. The effects of hypothermia on myocyte contractility were investigated under conditions of normal perfusion and after a 10. min interval of ischemia. Methods: Ventricular myocytes were obtained from 10 male Sprague-Dawley rats weighing 400±50g. The myocytes were randomized to be perfused at: 37°C, 34°C, 32°C, or 30°C. A subsequent set of myocytes was subjected to 10min of ischemia at 37°C, prior to being randomized to reperfusion at: 37°C, 34°C, 32°C or 30°C. Myocyte contractility was expressed as length-shortening percentage. Intracellular Ca2+ transients were assessed in a separate group of myocytes preloaded with Fura-2/AM. Sensitivity to Ca2+ was tested by increasing perfusate Ca2+ content, i.e. 0.5mM, 1mM and 2mM. Results: During normal perfusion and following reperfusion after 10min of ischemia, myocyte contractility increased at 34°C compared to 37°C (P2+ transients were greater during perfusion at 34°C compared to those at 37°C (P2+ concentration from 0.5mM to 2mM resulted in greater myocyte contractility during perfusion at 30°C compared to that observed at 37°C (P2+ transients and sensitivity to Ca2+ persisted after ischemia. Conclusions: Hypothermia improved myocyte contractility, intracellular Ca2+ transients and sensitivity to Ca2+ under conditions of normal perfusion and following reperfusion after 10min of ischemia.
| Original language | English |
|---|---|
| Pages (from-to) | 898-903 |
| Number of pages | 6 |
| Journal | Resuscitation |
| Volume | 81 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - Jul 2010 |
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Keywords
- Contractility
- Hypothermia
- Intracellular Ca
- Myocytes
ASJC Scopus subject areas
- Cardiology and Cardiovascular Medicine
- Emergency
- Emergency Medicine
- Medicine(all)
Cite this
Hypothermia improves ventricular myocyte contractility under conditions of normal perfusion and after an interval of ischemia. / Ristagno, Giuseppe; Tantillo, Simona; Sun, Shijie; Weil, Max Harry; Tang, Wanchun.
In: Resuscitation, Vol. 81, No. 7, 07.2010, p. 898-903.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Hypothermia improves ventricular myocyte contractility under conditions of normal perfusion and after an interval of ischemia
AU - Ristagno, Giuseppe
AU - Tantillo, Simona
AU - Sun, Shijie
AU - Weil, Max Harry
AU - Tang, Wanchun
PY - 2010/7
Y1 - 2010/7
N2 - Aim: Recent investigations have reported improved myocardial function during hypothermia following resuscitation from cardiac arrest. The effects of hypothermia on myocyte contractility were investigated under conditions of normal perfusion and after a 10. min interval of ischemia. Methods: Ventricular myocytes were obtained from 10 male Sprague-Dawley rats weighing 400±50g. The myocytes were randomized to be perfused at: 37°C, 34°C, 32°C, or 30°C. A subsequent set of myocytes was subjected to 10min of ischemia at 37°C, prior to being randomized to reperfusion at: 37°C, 34°C, 32°C or 30°C. Myocyte contractility was expressed as length-shortening percentage. Intracellular Ca2+ transients were assessed in a separate group of myocytes preloaded with Fura-2/AM. Sensitivity to Ca2+ was tested by increasing perfusate Ca2+ content, i.e. 0.5mM, 1mM and 2mM. Results: During normal perfusion and following reperfusion after 10min of ischemia, myocyte contractility increased at 34°C compared to 37°C (P2+ transients were greater during perfusion at 34°C compared to those at 37°C (P2+ concentration from 0.5mM to 2mM resulted in greater myocyte contractility during perfusion at 30°C compared to that observed at 37°C (P2+ transients and sensitivity to Ca2+ persisted after ischemia. Conclusions: Hypothermia improved myocyte contractility, intracellular Ca2+ transients and sensitivity to Ca2+ under conditions of normal perfusion and following reperfusion after 10min of ischemia.
AB - Aim: Recent investigations have reported improved myocardial function during hypothermia following resuscitation from cardiac arrest. The effects of hypothermia on myocyte contractility were investigated under conditions of normal perfusion and after a 10. min interval of ischemia. Methods: Ventricular myocytes were obtained from 10 male Sprague-Dawley rats weighing 400±50g. The myocytes were randomized to be perfused at: 37°C, 34°C, 32°C, or 30°C. A subsequent set of myocytes was subjected to 10min of ischemia at 37°C, prior to being randomized to reperfusion at: 37°C, 34°C, 32°C or 30°C. Myocyte contractility was expressed as length-shortening percentage. Intracellular Ca2+ transients were assessed in a separate group of myocytes preloaded with Fura-2/AM. Sensitivity to Ca2+ was tested by increasing perfusate Ca2+ content, i.e. 0.5mM, 1mM and 2mM. Results: During normal perfusion and following reperfusion after 10min of ischemia, myocyte contractility increased at 34°C compared to 37°C (P2+ transients were greater during perfusion at 34°C compared to those at 37°C (P2+ concentration from 0.5mM to 2mM resulted in greater myocyte contractility during perfusion at 30°C compared to that observed at 37°C (P2+ transients and sensitivity to Ca2+ persisted after ischemia. Conclusions: Hypothermia improved myocyte contractility, intracellular Ca2+ transients and sensitivity to Ca2+ under conditions of normal perfusion and following reperfusion after 10min of ischemia.
KW - Contractility
KW - Hypothermia
KW - Intracellular Ca
KW - Myocytes
UR - http://www.scopus.com/inward/record.url?scp=77953705444&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77953705444&partnerID=8YFLogxK
U2 - 10.1016/j.resuscitation.2010.03.016
DO - 10.1016/j.resuscitation.2010.03.016
M3 - Article
C2 - 20395031
AN - SCOPUS:77953705444
VL - 81
SP - 898
EP - 903
JO - Resuscitation
JF - Resuscitation
SN - 0300-9572
IS - 7
ER -