Hypoxia-induced electrical changes in striatal neurons

Research output: Contribution to journalArticle

Abstract

We have studied the effects of hypoxia on the membrane properties of striatal neurons intracellularly recorded from a corticostriatal slice preparation. Brief (2-10 min) periods of hypoxia produced reversible membrane depolarizations. Longer periods of hypoxia (12-20 min) produced irreversible membrane depolarizations. In voltage-clamp experiments, hypoxia caused an inward current coupled with an increased membrane conductance. Tetrodotoxin or low calcium (Ca2+)-high magnesium-containing solutions blocked synaptic transmission, but they did not reduce the hypoxia-induced electrical changes. Antagonists of excitatory amino acid (EAA) receptors failed to affect the electrical effects caused by oxygen (O2) deprivation. In low sodium (Na+)containing solutions the hypoxia-induced inward current was largely reduced. Blockade of ATP-dependent Na+-potassium (K+) pump by ouabain enhanced hypoxia-induced membrane depolarizations and/or inward currents. Our findings indicate that, at least for in vitro experiments, the release of EAAs is not required for the acute hypoxia-induced electrical changes in striatal neurons.

Original languageEnglish
Pages (from-to)1141-1145
Number of pages5
JournalJournal of Cerebral Blood Flow and Metabolism
Volume15
Issue number6
Publication statusPublished - 1995

Keywords

  • Excitatory amino acids
  • Hypoxia
  • Ischemia
  • Sodium-potassium ATP-dependent pump
  • Striatum
  • Synaptic transmission

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism
  • Neuroscience(all)

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