I-309 binds to and activates endothelial cell functions and acts as an angiogenic molecule in vivo

Giovanni Bernardini, Gaia Spinetti, Domenico Ribatti, Grazia Camarda, Lucia Morbidelli, Marina Ziche, Angela Santoni, Maurizio C. Capogrossi, Monica Napolitano

Research output: Contribution to journalArticlepeer-review

Abstract

Several chemokines have been shown to act as angiogenic molecules or to modulate the activity of growth factors such as fibroblast growth factor 2 (FGF-2) and vascular endothelial growth factor (VEGF). The detection of the CC chemokine receptor (CCR) 8 message in human umbilical vein endothelial cells (HUVECs) by reverse transcription-polymerase chain reaction (RT-PCR) and RNase protection assay (RPA), prompted us to investigate the potential role exerted by the CC chemokine I-309, a known ligand of such receptor, in both in vitro and in vivo angiogenesis assays. We show here that I-309 binds to endothelial cells, stimulates chemotaxis and invasion of these cells, and enhances HUVEC differentiation into capillary-like structures in an in vitro Matrigel assay. Furthermore, I-309 is an inducer of angiogenesis in vivo in both the rabbit cornea and the chick chorioallantoic membrane assay (CAM).

Original languageEnglish
Pages (from-to)4039-4045
Number of pages7
JournalBlood
Volume96
Issue number13
Publication statusPublished - 2000

ASJC Scopus subject areas

  • Hematology

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