Abstract
Ibuprofen, a chiral non-steroidal anti-inflammatory drug chemically related to fenoprofen and naproxen, has moderate but definite anti-inflammatory, analgesic and antipyretic properties, with considerably less gastrointestinal adverse effect than other drugs in the same family. Currently available in the market are preparations in which bioavailability of ibuprofen is increased by salification with various salts. We have investigated the pharmacological properties of one such salt, ibuprofen-arginine, of biological interest because l-arginine acts as substrate of the nitric oxide (NO) synthesising enzymes. Using epithelial HeLa cells expressing the endothelial NO synthase we show that ibuprofen-arginine releases NO and that this NO protects against the cytotoxic apoptogenic effects of staurosporine. We also found that ibuprofen-arginine is endowed with enhanced anti-inflammatory effects with respect to ibuprofen, as shown by reduced hind paw oedema, neutrophil infiltration and chondrocyte apoptosis in collagen-induced mouse arthritis, a model of chronic inflammation. NO has pleiotropic beneficial effects that may contribute to limit inflammation and anti-inflammatory compounds able to release NO display higher efficacy than the parent drugs in defined clinical settings. Our results open the possibility that NO generation contributes to the enhanced anti-inflammatory effects of ibuprofen-arginine vs. ibuprofen, suggesting co-administration of anti-inflammatory drugs and arginine as an additional way to exploit the beneficial effects of NO.
| Original language | English |
|---|---|
| Pages (from-to) | 221-228 |
| Number of pages | 8 |
| Journal | Pharmacological Research |
| Volume | 60 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Oct 2009 |
Fingerprint
Keywords
- Apoptosis
- Arginine
- Arthritis
- Ibuprofen
- Nitric oxide
- Salification
ASJC Scopus subject areas
- Pharmacology
Cite this
Ibuprofen-arginine generates nitric oxide and has enhanced anti-inflammatory effects. / De Palma, Clara; Di Paola, Rosanna; Perrotta, Cristiana; Mazzon, Emanuela; Cattaneo, Dario; Trabucchi, Emilio; Cuzzocrea, Salvatore; Clementi, Emilio.
In: Pharmacological Research, Vol. 60, No. 4, 10.2009, p. 221-228.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Ibuprofen-arginine generates nitric oxide and has enhanced anti-inflammatory effects
AU - De Palma, Clara
AU - Di Paola, Rosanna
AU - Perrotta, Cristiana
AU - Mazzon, Emanuela
AU - Cattaneo, Dario
AU - Trabucchi, Emilio
AU - Cuzzocrea, Salvatore
AU - Clementi, Emilio
PY - 2009/10
Y1 - 2009/10
N2 - Ibuprofen, a chiral non-steroidal anti-inflammatory drug chemically related to fenoprofen and naproxen, has moderate but definite anti-inflammatory, analgesic and antipyretic properties, with considerably less gastrointestinal adverse effect than other drugs in the same family. Currently available in the market are preparations in which bioavailability of ibuprofen is increased by salification with various salts. We have investigated the pharmacological properties of one such salt, ibuprofen-arginine, of biological interest because l-arginine acts as substrate of the nitric oxide (NO) synthesising enzymes. Using epithelial HeLa cells expressing the endothelial NO synthase we show that ibuprofen-arginine releases NO and that this NO protects against the cytotoxic apoptogenic effects of staurosporine. We also found that ibuprofen-arginine is endowed with enhanced anti-inflammatory effects with respect to ibuprofen, as shown by reduced hind paw oedema, neutrophil infiltration and chondrocyte apoptosis in collagen-induced mouse arthritis, a model of chronic inflammation. NO has pleiotropic beneficial effects that may contribute to limit inflammation and anti-inflammatory compounds able to release NO display higher efficacy than the parent drugs in defined clinical settings. Our results open the possibility that NO generation contributes to the enhanced anti-inflammatory effects of ibuprofen-arginine vs. ibuprofen, suggesting co-administration of anti-inflammatory drugs and arginine as an additional way to exploit the beneficial effects of NO.
AB - Ibuprofen, a chiral non-steroidal anti-inflammatory drug chemically related to fenoprofen and naproxen, has moderate but definite anti-inflammatory, analgesic and antipyretic properties, with considerably less gastrointestinal adverse effect than other drugs in the same family. Currently available in the market are preparations in which bioavailability of ibuprofen is increased by salification with various salts. We have investigated the pharmacological properties of one such salt, ibuprofen-arginine, of biological interest because l-arginine acts as substrate of the nitric oxide (NO) synthesising enzymes. Using epithelial HeLa cells expressing the endothelial NO synthase we show that ibuprofen-arginine releases NO and that this NO protects against the cytotoxic apoptogenic effects of staurosporine. We also found that ibuprofen-arginine is endowed with enhanced anti-inflammatory effects with respect to ibuprofen, as shown by reduced hind paw oedema, neutrophil infiltration and chondrocyte apoptosis in collagen-induced mouse arthritis, a model of chronic inflammation. NO has pleiotropic beneficial effects that may contribute to limit inflammation and anti-inflammatory compounds able to release NO display higher efficacy than the parent drugs in defined clinical settings. Our results open the possibility that NO generation contributes to the enhanced anti-inflammatory effects of ibuprofen-arginine vs. ibuprofen, suggesting co-administration of anti-inflammatory drugs and arginine as an additional way to exploit the beneficial effects of NO.
KW - Apoptosis
KW - Arginine
KW - Arthritis
KW - Ibuprofen
KW - Nitric oxide
KW - Salification
UR - http://www.scopus.com/inward/record.url?scp=69249221334&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=69249221334&partnerID=8YFLogxK
U2 - 10.1016/j.phrs.2009.06.002
DO - 10.1016/j.phrs.2009.06.002
M3 - Article
C2 - 19539763
AN - SCOPUS:69249221334
VL - 60
SP - 221
EP - 228
JO - Pharmacological Research
JF - Pharmacological Research
SN - 1043-6618
IS - 4
ER -