ICAM-1 expression and fluid phase endocytosis (FPE) in cultured brain microvascular endothelial cells (BMECs) after exposure to recombinant Interferon β-la (IFβ-la) and TNFa

G. Defazio, M. Trojano, D. Ribatti, B. Nico, K. DeSalvia, M. Giorelli, G. Russo, L. Roncali, P. Livrea

Research output: Contribution to journalArticlepeer-review

Abstract

Treatment of relapsing-remitting (RR) multiple sclerosis (MS) with IFβ-la reduced relapse rate and gadolinium-enhanced MRI lesions (Jacobs et al, 1996). Cytokines like TNFa may contributr to blood brain barrier (BBB) breakdown which is associated with increased FPE and ICAM-1 expression by BMECs. We examined whether IFNβ-la affected ICAM-1 expression and FPE of horseradish peroxidase in cultured rat BMECs stimulated by TNF a. Treatment of BMECs monolayer with TNFa (100 U/ml) for 24 h significantly increased ICAM-1 expression and FPE, whereas 100-1000 U/ml EFNβ-la for 72 h did not modify baseline ICAM-1 and FPE levels. Incubation of BMEC with 1000 U/ml IFNβ-la for 48 h followed by combined IFβ -la and TNFa for 24 h significantly downregulated TNFa-induced elevation of ICAM 1 and FPE. The therapeutic effect of IFNβ-la on RR MS may involve direct regulatory actions on BMECs.

Original languageEnglish
Pages (from-to)33
Number of pages1
JournalItalian Journal of Neurological Sciences
Volume18
Issue number4
Publication statusPublished - 1997

ASJC Scopus subject areas

  • Neuroscience(all)
  • Clinical Neurology

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