ICAM 1 expression and fluid phase endocytosis of cultured brain microvascular endothelial cells following exposure to interferon β-1a and TNFα

G. Defazio, M. Trojano, D. Ribatti, B. Nico, M. Giorelli, R. De Salvia, G. Russo, L. Roncali, P. Livrea

Research output: Contribution to journalArticle


We have studied the effect of interferon (IF) β-1a on the basal and TNFα-induced intercellular adhesion molecule 1 (ICAM 1) expression and fluid phase endocytosis (FPE) of horseradish peroxidase in cultured rat brain microvascular endothelial cells. Neither basal ICAM 1 expression nor basal FPE were significantly affected by 24-72 h exposure to 1000 U/ml IFβ-1a. ICAM 1 induction and FPE enhancement caused by 100 U/ml TNFα for 24 h was not influenced by simultaneous administration of 1000 U/ml IFβ-1a. Treatment of cultures with IFβ-1a for 48 h followed by 24-h coincubation with TNFα (100 U/ml) and IFβ-1a (1000 U/ml) resulted in significant downregulation of TNFα-induced ICAM 1 expression and FPE. Downregulation of TNFα-induced ICAM 1 expression was not observed when combined treatment with TNFα (100 U/ml) and IFβ-1a (1000 U/ml) for 24 h was followed by 48 h exposure to IFβ-1a. We concluded that the blood-brain barrier endothelium may be a target of IFβ- 1a. Further, these in vitro findings may correlate with the results of recent clinical trials indicating that chronic treatment of relapsing remitting multiple sclerosis with IFβ-1a prevents both clinical exacerbations and the appearance on Magnetic Resonance Imaging of new lesions enhanced by gadolinium which is taken up by increased transendothelial fluid phase vesicular transport.

Original languageEnglish
Pages (from-to)13-20
Number of pages8
JournalJournal of Neuroimmunology
Issue number1-2
Publication statusPublished - Aug 1 1998



  • Brain microvascular endothelial cell culture
  • Fluid phase endocytosis
  • ICAM 1
  • Interferon β
  • Multiple sclerosis
  • Tumor necrosis factor α

ASJC Scopus subject areas

  • Immunology
  • Clinical Neurology
  • Immunology and Allergy
  • Neurology

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