Abstract
We have studied the effect of interferon (IF) β-1a on the basal and TNFα-induced intercellular adhesion molecule 1 (ICAM 1) expression and fluid phase endocytosis (FPE) of horseradish peroxidase in cultured rat brain microvascular endothelial cells. Neither basal ICAM 1 expression nor basal FPE were significantly affected by 24-72 h exposure to 1000 U/ml IFβ-1a. ICAM 1 induction and FPE enhancement caused by 100 U/ml TNFα for 24 h was not influenced by simultaneous administration of 1000 U/ml IFβ-1a. Treatment of cultures with IFβ-1a for 48 h followed by 24-h coincubation with TNFα (100 U/ml) and IFβ-1a (1000 U/ml) resulted in significant downregulation of TNFα-induced ICAM 1 expression and FPE. Downregulation of TNFα-induced ICAM 1 expression was not observed when combined treatment with TNFα (100 U/ml) and IFβ-1a (1000 U/ml) for 24 h was followed by 48 h exposure to IFβ-1a. We concluded that the blood-brain barrier endothelium may be a target of IFβ- 1a. Further, these in vitro findings may correlate with the results of recent clinical trials indicating that chronic treatment of relapsing remitting multiple sclerosis with IFβ-1a prevents both clinical exacerbations and the appearance on Magnetic Resonance Imaging of new lesions enhanced by gadolinium which is taken up by increased transendothelial fluid phase vesicular transport.
| Original language | English |
|---|---|
| Pages (from-to) | 13-20 |
| Number of pages | 8 |
| Journal | Journal of Neuroimmunology |
| Volume | 88 |
| Issue number | 1-2 |
| DOIs | |
| Publication status | Published - Aug 1 1998 |
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Keywords
- Brain microvascular endothelial cell culture
- Fluid phase endocytosis
- ICAM 1
- Interferon β
- Multiple sclerosis
- Tumor necrosis factor α
ASJC Scopus subject areas
- Immunology
- Clinical Neurology
- Immunology and Allergy
- Neurology
Cite this
ICAM 1 expression and fluid phase endocytosis of cultured brain microvascular endothelial cells following exposure to interferon β-1a and TNFα. / Defazio, G.; Trojano, M.; Ribatti, D.; Nico, B.; Giorelli, M.; De Salvia, R.; Russo, G.; Roncali, L.; Livrea, P.
In: Journal of Neuroimmunology, Vol. 88, No. 1-2, 01.08.1998, p. 13-20.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - ICAM 1 expression and fluid phase endocytosis of cultured brain microvascular endothelial cells following exposure to interferon β-1a and TNFα
AU - Defazio, G.
AU - Trojano, M.
AU - Ribatti, D.
AU - Nico, B.
AU - Giorelli, M.
AU - De Salvia, R.
AU - Russo, G.
AU - Roncali, L.
AU - Livrea, P.
PY - 1998/8/1
Y1 - 1998/8/1
N2 - We have studied the effect of interferon (IF) β-1a on the basal and TNFα-induced intercellular adhesion molecule 1 (ICAM 1) expression and fluid phase endocytosis (FPE) of horseradish peroxidase in cultured rat brain microvascular endothelial cells. Neither basal ICAM 1 expression nor basal FPE were significantly affected by 24-72 h exposure to 1000 U/ml IFβ-1a. ICAM 1 induction and FPE enhancement caused by 100 U/ml TNFα for 24 h was not influenced by simultaneous administration of 1000 U/ml IFβ-1a. Treatment of cultures with IFβ-1a for 48 h followed by 24-h coincubation with TNFα (100 U/ml) and IFβ-1a (1000 U/ml) resulted in significant downregulation of TNFα-induced ICAM 1 expression and FPE. Downregulation of TNFα-induced ICAM 1 expression was not observed when combined treatment with TNFα (100 U/ml) and IFβ-1a (1000 U/ml) for 24 h was followed by 48 h exposure to IFβ-1a. We concluded that the blood-brain barrier endothelium may be a target of IFβ- 1a. Further, these in vitro findings may correlate with the results of recent clinical trials indicating that chronic treatment of relapsing remitting multiple sclerosis with IFβ-1a prevents both clinical exacerbations and the appearance on Magnetic Resonance Imaging of new lesions enhanced by gadolinium which is taken up by increased transendothelial fluid phase vesicular transport.
AB - We have studied the effect of interferon (IF) β-1a on the basal and TNFα-induced intercellular adhesion molecule 1 (ICAM 1) expression and fluid phase endocytosis (FPE) of horseradish peroxidase in cultured rat brain microvascular endothelial cells. Neither basal ICAM 1 expression nor basal FPE were significantly affected by 24-72 h exposure to 1000 U/ml IFβ-1a. ICAM 1 induction and FPE enhancement caused by 100 U/ml TNFα for 24 h was not influenced by simultaneous administration of 1000 U/ml IFβ-1a. Treatment of cultures with IFβ-1a for 48 h followed by 24-h coincubation with TNFα (100 U/ml) and IFβ-1a (1000 U/ml) resulted in significant downregulation of TNFα-induced ICAM 1 expression and FPE. Downregulation of TNFα-induced ICAM 1 expression was not observed when combined treatment with TNFα (100 U/ml) and IFβ-1a (1000 U/ml) for 24 h was followed by 48 h exposure to IFβ-1a. We concluded that the blood-brain barrier endothelium may be a target of IFβ- 1a. Further, these in vitro findings may correlate with the results of recent clinical trials indicating that chronic treatment of relapsing remitting multiple sclerosis with IFβ-1a prevents both clinical exacerbations and the appearance on Magnetic Resonance Imaging of new lesions enhanced by gadolinium which is taken up by increased transendothelial fluid phase vesicular transport.
KW - Brain microvascular endothelial cell culture
KW - Fluid phase endocytosis
KW - ICAM 1
KW - Interferon β
KW - Multiple sclerosis
KW - Tumor necrosis factor α
UR - http://www.scopus.com/inward/record.url?scp=0032146241&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032146241&partnerID=8YFLogxK
U2 - 10.1016/S0165-5728(98)00064-2
DO - 10.1016/S0165-5728(98)00064-2
M3 - Article
C2 - 9688319
AN - SCOPUS:0032146241
VL - 88
SP - 13
EP - 20
JO - Journal of Neuroimmunology
JF - Journal of Neuroimmunology
SN - 0165-5728
IS - 1-2
ER -