Identification and characterization of a κB/Rel binding site in the regulatory region of the amyloid precursor protein gene

M. Grilli, M. Ribola, A. Alberici, A. Valerio, M. Memo, P. Spano

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Abstract

Several observations support the hypothesis that pathogenetic mechanisms of β amyloid formation in Alzheimer's disease may involve alterations in amyloid precursor protein (APP) gene expression. In this regard, molecular dissection of the APP gene transcriptional regulation is of primary importance. We report evidence that members of the family of transcription factors NFκB/Rel can specifically recognize two identical sequences located in the 5'-regulatory region of APP. These sequences, which we refer to as APPκB sites, interact preferentially with p50-containing members of the family. In particular, p50 homodimers and p50/p65 and p50/c-Rel heterodimers act as transcriptional activators at the APPκB site. Finally, the nuclear complex specifically binding to the APPκR sites proves to be an integral part of neurons and lymphocytes.

Original languageEnglish
Pages (from-to)26774-26777
Number of pages4
JournalJournal of Biological Chemistry
Volume270
Issue number45
DOIs
Publication statusPublished - 1995

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Amyloid beta-Protein Precursor
Nucleic Acid Regulatory Sequences
Genes
Binding Sites
Gene expression
Dissection
Lymphocytes
Amyloid
Neurons
Alzheimer Disease
Transcription Factors
Gene Expression

ASJC Scopus subject areas

  • Biochemistry

Cite this

Identification and characterization of a κB/Rel binding site in the regulatory region of the amyloid precursor protein gene. / Grilli, M.; Ribola, M.; Alberici, A.; Valerio, A.; Memo, M.; Spano, P.

In: Journal of Biological Chemistry, Vol. 270, No. 45, 1995, p. 26774-26777.

Research output: Contribution to journalArticle

Grilli, M. ; Ribola, M. ; Alberici, A. ; Valerio, A. ; Memo, M. ; Spano, P. / Identification and characterization of a κB/Rel binding site in the regulatory region of the amyloid precursor protein gene. In: Journal of Biological Chemistry. 1995 ; Vol. 270, No. 45. pp. 26774-26777.
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