Identification and characterization of adult stem/progenitor cells in the human bladder (bladder spheroids): perspectives of application in pediatric surgery.

Alessandra Fierabracci, Paolo Caione, Monica Di Giovine, Daniela Zavaglia, Gian Franco Bottazzo

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

There is evidence that tissue-specific stem cells reside in certain adult tissues. Their specific properties remain elusive, because they are rare in parent tissues and heterogeneous; furthermore, technical difficulties have been encountered in their identification and the characterization of their progeny. The aim of this study was to isolate stem/progenitor cells from the human bladder. We have devised a method for isolating stem/progenitor cells from the human bladder. This is based on the enzymatic digestion of fresh surgical bladder specimens, followed by culture of cells in the presence of EGF and bFGF. We also used markers that identified and finally characterized these cells. Spheroids with self-replicative potential were obtained from all bladder specimens. The isolated population contained a subset of CD34+ CD45- cells. These spheroids represent a predominant functional type of stem/progenitor cells within the human bladder. This envisage their potential use for the treatment of animal models in pediatric surgery.

Original languageEnglish
Pages (from-to)837-839
Number of pages3
JournalPediatric Surgery International
Volume23
Issue number9
DOIs
Publication statusPublished - Sep 2007

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Adult Stem Cells
Urinary Bladder
Stem Cells
Pediatrics
Epidermal Growth Factor
Digestion
Animal Models
Cell Culture Techniques
Population

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

Cite this

Identification and characterization of adult stem/progenitor cells in the human bladder (bladder spheroids) : perspectives of application in pediatric surgery. / Fierabracci, Alessandra; Caione, Paolo; Di Giovine, Monica; Zavaglia, Daniela; Bottazzo, Gian Franco.

In: Pediatric Surgery International, Vol. 23, No. 9, 09.2007, p. 837-839.

Research output: Contribution to journalArticle

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