Identification and characterization of CDS2, a mammalian homolog of the Drosophila CDP-diacylglycerol synthase gene

M. Volta, A. Bulfone, C. Gattuso, E. Rossi, M. Mariani, G. G. Consalez, O. Zuffardi, A. Ballabio, S. Banfi, B. Franco

Research output: Contribution to journalArticle

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Abstract

The general strategies of phototransduction in vertebrates and invertebrates share many similarities, but differ significantly in their underlying molecular machinery. The CDS gene encodes the CDP-diacylglycerol synthase (CDS) enzyme and is required for phototransduction in Drosophila. Using a bioinformatic approach, we have identified two novel transcripts (CDS1 and CDS2) highly homologous to the Drosophila CDS gene. We isolated and sequenced the CDS2 full-length cDNA and mapped the two genes to human chromosomes 20p13 (CDS2) and 4q21.1 (CDS1). Sequence analysis revealed that both genes are highly homologous to the Drosophila protein (64.4 and 58.6% identity at the protein level between CDS and CDS2 and between CDS and CDS1, respectively). The mouse homologs for both genes were isolated and used in RNA in situ hybridization studies on adult and embryonic mouse tissue sections. These studies showed that Cds2 is highly expressed in the differentiating neuroblasts of the neural retina and in the central nervous system during embryonic development, while it was not detected in adult retina. Cds1, on the other hand, shows a high level of expression in the photoreceptor layer of adult retina, which strongly suggests a role for Cds1 in phototransduction. Knowledge of the expression pattern of these genes in mammals may shed light on the evolution of vision mechanisms and help in the evaluation of candidate genes for human retinopathies.

Original languageEnglish
Pages (from-to)68-77
Number of pages10
JournalGenomics
Volume55
Issue number1
DOIs
Publication statusPublished - Jan 1 1999

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Diacylglycerol Cholinephosphotransferase
Drosophila
Light Signal Transduction
Genes
Retina
Human Chromosomes
Invertebrates
Computational Biology
Embryonic Development
In Situ Hybridization
Sequence Analysis
Vertebrates
Mammals
Central Nervous System
Complementary DNA
RNA
Gene Expression
Enzymes

ASJC Scopus subject areas

  • Genetics

Cite this

Identification and characterization of CDS2, a mammalian homolog of the Drosophila CDP-diacylglycerol synthase gene. / Volta, M.; Bulfone, A.; Gattuso, C.; Rossi, E.; Mariani, M.; Consalez, G. G.; Zuffardi, O.; Ballabio, A.; Banfi, S.; Franco, B.

In: Genomics, Vol. 55, No. 1, 01.01.1999, p. 68-77.

Research output: Contribution to journalArticle

Volta, M, Bulfone, A, Gattuso, C, Rossi, E, Mariani, M, Consalez, GG, Zuffardi, O, Ballabio, A, Banfi, S & Franco, B 1999, 'Identification and characterization of CDS2, a mammalian homolog of the Drosophila CDP-diacylglycerol synthase gene', Genomics, vol. 55, no. 1, pp. 68-77. https://doi.org/10.1006/geno.1998.5610
Volta, M. ; Bulfone, A. ; Gattuso, C. ; Rossi, E. ; Mariani, M. ; Consalez, G. G. ; Zuffardi, O. ; Ballabio, A. ; Banfi, S. ; Franco, B. / Identification and characterization of CDS2, a mammalian homolog of the Drosophila CDP-diacylglycerol synthase gene. In: Genomics. 1999 ; Vol. 55, No. 1. pp. 68-77.
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