Identification and characterization of ZFP-57, a novel zinc finger transcription factor in the mammalian peripheral nervous system

María B Durán Alonso, Georg Zoid, Carla Taveggia, Frank Bosse, Christiane Zoidl, Mary Rahman, Eric Parmantier, Charlotte H. Dean, Brett S. Harris, Lawrence Wrabetz, Hans Werner Müller, Kristjan R. Jessen, Rhona Mirsky

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

To isolate new zinc finger genes expressed at early stages of peripheral nerve development, we have used PCR to amplify conserved zinc finger sequences. RNA from rat embryonic day 12 and 13 sciatic nerves, a stage when nerves contain Schwann cell precursors, was used to identify several genes not previously described in Schwann cells. One of them, zinc finger protein (ZFP)-57, proved to be the homologue of a mouse gene found in F9 teratocarcinoma cells. Its mRNA expression profile within embryonic and adult normal and transected peripheral nerves, and its distribution in the rest of the nervous system is described. High levels of expression are seen in embryonic nerves and spinal cord. These drop rapidly during the first few weeks after birth, a pattern mirrored in other parts of the nervous system. ZFP-57 localizes to the nucleus of Schwann and other cells. The sequence contains an N-terminal Krüppel-associated box (KRAB) domain and ZFP-57 constructs containing green fluorescent protein reveal that the protein colocalizes with heterochromatin protein 1a to centromeric heterochromatin in a characteristic speckled pattern in NIH3T3 cells. The KRAB domain is required for this localization, because constructs lacking it target the protein to the nucleus but not to the centromeric heterochromatin. When fused to a heterologous DNA binding domain, the KRAB domain of ZFP-57 represses transcription, and full-length ZFP-57 represses Schwann cell transcription from myelin basic protein and P 0 promoters in co-transfection assays. Zfp-57 mRNA is up-regulated in Schwann cells in response to leukemia inhibitory factor and fibroblast growth factor 2.

Original languageEnglish
Pages (from-to)25653-25664
Number of pages12
JournalJournal of Biological Chemistry
Volume279
Issue number24
DOIs
Publication statusPublished - Jun 11 2004

Fingerprint

Zinc Fingers
Peripheral Nervous System
Neurology
Zinc
Transcription Factors
Schwann Cells
Heterochromatin
Proteins
Cells
Genes
Transcription
Peripheral Nerves
Nervous System
Teratocarcinoma
Leukemia Inhibitory Factor
Messenger RNA
Myelin Basic Protein
Fibroblast Growth Factor 2
Sciatic Nerve
Green Fluorescent Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Identification and characterization of ZFP-57, a novel zinc finger transcription factor in the mammalian peripheral nervous system. / Alonso, María B Durán; Zoid, Georg; Taveggia, Carla; Bosse, Frank; Zoidl, Christiane; Rahman, Mary; Parmantier, Eric; Dean, Charlotte H.; Harris, Brett S.; Wrabetz, Lawrence; Müller, Hans Werner; Jessen, Kristjan R.; Mirsky, Rhona.

In: Journal of Biological Chemistry, Vol. 279, No. 24, 11.06.2004, p. 25653-25664.

Research output: Contribution to journalArticle

Alonso, MBD, Zoid, G, Taveggia, C, Bosse, F, Zoidl, C, Rahman, M, Parmantier, E, Dean, CH, Harris, BS, Wrabetz, L, Müller, HW, Jessen, KR & Mirsky, R 2004, 'Identification and characterization of ZFP-57, a novel zinc finger transcription factor in the mammalian peripheral nervous system', Journal of Biological Chemistry, vol. 279, no. 24, pp. 25653-25664. https://doi.org/10.1074/jbc.M400415200
Alonso, María B Durán ; Zoid, Georg ; Taveggia, Carla ; Bosse, Frank ; Zoidl, Christiane ; Rahman, Mary ; Parmantier, Eric ; Dean, Charlotte H. ; Harris, Brett S. ; Wrabetz, Lawrence ; Müller, Hans Werner ; Jessen, Kristjan R. ; Mirsky, Rhona. / Identification and characterization of ZFP-57, a novel zinc finger transcription factor in the mammalian peripheral nervous system. In: Journal of Biological Chemistry. 2004 ; Vol. 279, No. 24. pp. 25653-25664.
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