Abstract
The human MAGE-3 gene encodes a melanoma antigenic epitope recognized by specific cytotoxic T lymphocytes, but its gene product has not been identified thus far. We produced a recombinant MAGE-3 gene product by expression cloning of the entire reading frame in the context of a fusion protein characterized by a 10-histidine tail, allowing purification by metal chelation on a nickel Sepharose column. The semipurified product was used to generate MAGE-3-specific monoclonal antibodies. One reagent could identify by immunoblotting the native MAGE-3 gene product as a M(r) 48,000 protein in lysates of cell lines showing evidence of MAGE-3 gene expression. No apparent cross-reactivity with recombinant or native MAGE-1 gene product was observed. Immunohistochemistry shows that, closely resembling the MAGE-1 gene product, MAGE-3 is a cytoplasmic protein.
| Original language | English |
|---|---|
| Pages (from-to) | 2236-2239 |
| Number of pages | 4 |
| Journal | Cancer Research |
| Volume | 55 |
| Issue number | 11 |
| Publication status | Published - 1995 |
Fingerprint
ASJC Scopus subject areas
- Cancer Research
- Oncology
Cite this
Identification and intracellular location of MAGE-3 gene product. / Kocher, T.; Schultz-Thater, E.; Gudat, F.; Schaefer, C.; Casorati, G.; Juretic, A.; Willimann, T.; Harder, F.; Heberer, M.; Spagnoli, G. C.
In: Cancer Research, Vol. 55, No. 11, 1995, p. 2236-2239.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Identification and intracellular location of MAGE-3 gene product
AU - Kocher, T.
AU - Schultz-Thater, E.
AU - Gudat, F.
AU - Schaefer, C.
AU - Casorati, G.
AU - Juretic, A.
AU - Willimann, T.
AU - Harder, F.
AU - Heberer, M.
AU - Spagnoli, G. C.
PY - 1995
Y1 - 1995
N2 - The human MAGE-3 gene encodes a melanoma antigenic epitope recognized by specific cytotoxic T lymphocytes, but its gene product has not been identified thus far. We produced a recombinant MAGE-3 gene product by expression cloning of the entire reading frame in the context of a fusion protein characterized by a 10-histidine tail, allowing purification by metal chelation on a nickel Sepharose column. The semipurified product was used to generate MAGE-3-specific monoclonal antibodies. One reagent could identify by immunoblotting the native MAGE-3 gene product as a M(r) 48,000 protein in lysates of cell lines showing evidence of MAGE-3 gene expression. No apparent cross-reactivity with recombinant or native MAGE-1 gene product was observed. Immunohistochemistry shows that, closely resembling the MAGE-1 gene product, MAGE-3 is a cytoplasmic protein.
AB - The human MAGE-3 gene encodes a melanoma antigenic epitope recognized by specific cytotoxic T lymphocytes, but its gene product has not been identified thus far. We produced a recombinant MAGE-3 gene product by expression cloning of the entire reading frame in the context of a fusion protein characterized by a 10-histidine tail, allowing purification by metal chelation on a nickel Sepharose column. The semipurified product was used to generate MAGE-3-specific monoclonal antibodies. One reagent could identify by immunoblotting the native MAGE-3 gene product as a M(r) 48,000 protein in lysates of cell lines showing evidence of MAGE-3 gene expression. No apparent cross-reactivity with recombinant or native MAGE-1 gene product was observed. Immunohistochemistry shows that, closely resembling the MAGE-1 gene product, MAGE-3 is a cytoplasmic protein.
UR - http://www.scopus.com/inward/record.url?scp=0029069090&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029069090&partnerID=8YFLogxK
M3 - Article
C2 - 7757970
AN - SCOPUS:0029069090
VL - 55
SP - 2236
EP - 2239
JO - Journal of Cancer Research
JF - Journal of Cancer Research
SN - 0008-5472
IS - 11
ER -