Identification and intracellular location of MAGE-3 gene product

T. Kocher, E. Schultz-Thater, F. Gudat, C. Schaefer, G. Casorati, A. Juretic, T. Willimann, F. Harder, M. Heberer, G. C. Spagnoli

Research output: Contribution to journalArticle

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Abstract

The human MAGE-3 gene encodes a melanoma antigenic epitope recognized by specific cytotoxic T lymphocytes, but its gene product has not been identified thus far. We produced a recombinant MAGE-3 gene product by expression cloning of the entire reading frame in the context of a fusion protein characterized by a 10-histidine tail, allowing purification by metal chelation on a nickel Sepharose column. The semipurified product was used to generate MAGE-3-specific monoclonal antibodies. One reagent could identify by immunoblotting the native MAGE-3 gene product as a M(r) 48,000 protein in lysates of cell lines showing evidence of MAGE-3 gene expression. No apparent cross-reactivity with recombinant or native MAGE-1 gene product was observed. Immunohistochemistry shows that, closely resembling the MAGE-1 gene product, MAGE-3 is a cytoplasmic protein.

Original languageEnglish
Pages (from-to)2236-2239
Number of pages4
JournalCancer Research
Volume55
Issue number11
Publication statusPublished - 1995

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Genes
Gene Expression
Reading Frames
Proteins
Cytotoxic T-Lymphocytes
Nickel
Immunoblotting
Histidine
Sepharose
Tail
Organism Cloning
Epitopes
Melanoma
Metals
Immunohistochemistry
Monoclonal Antibodies
Cell Line

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Kocher, T., Schultz-Thater, E., Gudat, F., Schaefer, C., Casorati, G., Juretic, A., ... Spagnoli, G. C. (1995). Identification and intracellular location of MAGE-3 gene product. Cancer Research, 55(11), 2236-2239.

Identification and intracellular location of MAGE-3 gene product. / Kocher, T.; Schultz-Thater, E.; Gudat, F.; Schaefer, C.; Casorati, G.; Juretic, A.; Willimann, T.; Harder, F.; Heberer, M.; Spagnoli, G. C.

In: Cancer Research, Vol. 55, No. 11, 1995, p. 2236-2239.

Research output: Contribution to journalArticle

Kocher, T, Schultz-Thater, E, Gudat, F, Schaefer, C, Casorati, G, Juretic, A, Willimann, T, Harder, F, Heberer, M & Spagnoli, GC 1995, 'Identification and intracellular location of MAGE-3 gene product', Cancer Research, vol. 55, no. 11, pp. 2236-2239.
Kocher T, Schultz-Thater E, Gudat F, Schaefer C, Casorati G, Juretic A et al. Identification and intracellular location of MAGE-3 gene product. Cancer Research. 1995;55(11):2236-2239.
Kocher, T. ; Schultz-Thater, E. ; Gudat, F. ; Schaefer, C. ; Casorati, G. ; Juretic, A. ; Willimann, T. ; Harder, F. ; Heberer, M. ; Spagnoli, G. C. / Identification and intracellular location of MAGE-3 gene product. In: Cancer Research. 1995 ; Vol. 55, No. 11. pp. 2236-2239.
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