Identification and mapping of human cDNAs homologous to Drosophila mutant genes through EST database searching

Sandro Banfi, Giuseppe Borsani, Elena Rossi, Loris Bernard, Alessandro Guffanti, Francesca Rubboli, Anna Marchitiello, Sabrina Giglio, Elisabetta Coluccia, Massimo Zollo, Orsetta Zuffardi, Andrea Ballabio

Research output: Contribution to journalArticle

Abstract

Cross-species comparison is an effective tool used to identify genes and study their function in both normal and pathological conditions. We have applied the power of Drosophila genetics to the vast resource of human cDNAs represented in the expressed sequence tag (EST) database (dbEST) to identify novel human genes of high biological interest. Sixty-six human cDNAs showing significant homology to genes causing Drosophila mutant phenotypes were identified by screening dbEST using the 'text string' option, and their map position was determined using both fluorescence in situ hybridization (FISH) and radiation hybrid mapping. Comparison between these genes and their putative partners in Drosophila may provide important insights into their function in mammals. Furthermore, integration of these genes into the transcription map of the human genome contributes to the positional candidate approach for disease gene identification.

Original languageEnglish
Pages (from-to)167-174
Number of pages8
JournalNature Genetics
Volume13
Issue number2
DOIs
Publication statusPublished - 1996

ASJC Scopus subject areas

  • Genetics(clinical)
  • Genetics

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    Banfi, S., Borsani, G., Rossi, E., Bernard, L., Guffanti, A., Rubboli, F., Marchitiello, A., Giglio, S., Coluccia, E., Zollo, M., Zuffardi, O., & Ballabio, A. (1996). Identification and mapping of human cDNAs homologous to Drosophila mutant genes through EST database searching. Nature Genetics, 13(2), 167-174. https://doi.org/10.1038/ng0696-167