Identification and molecular cloning of p75/AIRM1, a novel member of the sialoadhesin family that functions as an inhibitory receptor in human natural killer cells

Michela Falco, Roberto Biassoni, Cristina Bottino, Massimo Vitale, Simona Sivori, Raffaella Augugliaro, Lorenzo Moretta, Alessandro Moretta

Research output: Contribution to journalArticlepeer-review

Abstract

In this study, by the generation of a specific monoclonal antibody, we identified p75/AIRM1 (for adhesion inhibitory receptor molecule 1), a novel inhibitory receptor that is mostly confined to human natural killer cells, p75/AIRM1 is a 75-kD glycoprotein that, upon sodium pervanadate treatment, becomes tyrosine phosphorylated and associates to src homology 2 domain- bearing protein tyrosine phosphatase (SHP)-1. The p75/AIRM1 gene is located on human chromosome 19 and encodes a novel member of the sialoadhesin family characterized by three immunoglobulin-like extracellular domains (one NH2- terminal V-type and two C2-type) and a classical immunoreceptor tyrosine- based inhibitory motif (ITIM) in the cytoplasmic portion. The highest amino acid sequence similarity has been found with the myeloid-specific CD33 molecule and the placental CD33L1 protein. Similar to other sialoadhesin molecules, p75/AIRM1 appears to mediate sialic acid-dependent ligand recognition.

Original languageEnglish
Pages (from-to)793-801
Number of pages9
JournalJournal of Experimental Medicine
Volume190
Issue number6
DOIs
Publication statusPublished - Sep 20 1999

Keywords

  • Immunoglobulin superfamily
  • Inhibitory receptor
  • Molecular cloning
  • Natural killer cells
  • Sialoadhesin

ASJC Scopus subject areas

  • Immunology

Fingerprint Dive into the research topics of 'Identification and molecular cloning of p75/AIRM1, a novel member of the sialoadhesin family that functions as an inhibitory receptor in human natural killer cells'. Together they form a unique fingerprint.

Cite this