Identification and predictive value of interleukin-6+ interleukin-10+ and interleukin-6-interleukin-10+ cytokine patterns in st-elevation acute myocardial infarction

Enrico Ammirati, Carlo V. Cannistraci, Nicole A. Cristell, Viviana Vecchio, Alessio G. Palini, Per Tornvall, Anna M. Paganoni, Ewa A. Miendlarzewska, Laura M. Sangalli, Alberto Monello, John Pernow, Marie Björnstedt Bennermo, Giancarlo Marenzi, Dayi Hu, Neal G. Uren, Domenico Cianflone, Timothy Ravasi, Angelo A. Manfredi, Attilio Maseri

Research output: Contribution to journalArticle

Abstract

RATIONALE: At the onset of ST-elevation acute myocardial infarction (STEMI), patients can present with very high circulating interleukin-6 (IL-6) levels or very low-IL-6 levels. OBJECTIVE: We compared these 2 groups of patients to understand whether it is possible to define specific STEMI phenotypes associated with outcome based on the cytokine response. METHODS AND RESULTS: We compared 109 patients with STEMI in the top IL-6 level (median, 15.6 pg/mL; IL-6 STEMI) with 96 in the bottom IL-6 level (median, 1.7 pg/mL; IL-6 STEMI) and 103 matched controls extracted from the multiethnic First Acute Myocardial Infarction study. We found minimal clinical differences between IL-6 STEMI and IL-6 STEMI. We assessed the inflammatory profiles of the 2 STEMI groups and the controls by measuring 18 cytokines in blood samples. We exploited clustering analysis algorithms to infer the functional modules of interacting cytokines. IL-6 STEMI patients were characterized by the activation of 2 modules of interacting signals comprising IL-10, IL-8, macrophage inflammatory protein-1α, and C-reactive protein, and monocyte chemoattractant protein-1, macrophage inflammatory protein-1β, and monokine induced by interferon-γ. IL-10 was increased both in IL-6 STEMI and IL-6 STEMI patients compared with controls. IL-6IL-10 STEMI patients had an increased risk of systolic dysfunction at discharge and an increased risk of death at 6 months in comparison with IL-6IL-10 STEMI patients. We combined IL-10 and monokine induced by interferon-γ (derived from the 2 identified cytokine modules) with IL-6 in a formula yielding a risk index that outperformed any single cytokine in the prediction of systolic dysfunction and death. CONCLUSIONS: We have identified a characteristic circulating inflammatory cytokine pattern in STEMI patients, which is not related to the extent of myocardial damage. The simultaneous elevation of IL-6 and IL-10 levels distinguishes STEMI patients with worse clinical outcomes from other STEMI patients. These observations could have potential implications for risk-oriented patient stratification and immune-modulating therapies.

Original languageEnglish
Pages (from-to)1336-1348
Number of pages13
JournalCirculation Research
Volume111
Issue number10
DOIs
Publication statusPublished - Oct 26 2012

Fingerprint

Interleukin-10
Interleukin-6
Myocardial Infarction
Cytokines
Monokines
Macrophage Inflammatory Proteins
ST Elevation Myocardial Infarction
Interferons
Chemokine CCL2
Protein C
Interleukin-8
C-Reactive Protein
Cluster Analysis

Keywords

  • acute myocardial infarction
  • bioengineering
  • computational and systems biology
  • cytokines
  • inflammation
  • interleukin-10
  • interleukin-6

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Identification and predictive value of interleukin-6+ interleukin-10+ and interleukin-6-interleukin-10+ cytokine patterns in st-elevation acute myocardial infarction. / Ammirati, Enrico; Cannistraci, Carlo V.; Cristell, Nicole A.; Vecchio, Viviana; Palini, Alessio G.; Tornvall, Per; Paganoni, Anna M.; Miendlarzewska, Ewa A.; Sangalli, Laura M.; Monello, Alberto; Pernow, John; Björnstedt Bennermo, Marie; Marenzi, Giancarlo; Hu, Dayi; Uren, Neal G.; Cianflone, Domenico; Ravasi, Timothy; Manfredi, Angelo A.; Maseri, Attilio.

In: Circulation Research, Vol. 111, No. 10, 26.10.2012, p. 1336-1348.

Research output: Contribution to journalArticle

Ammirati, E, Cannistraci, CV, Cristell, NA, Vecchio, V, Palini, AG, Tornvall, P, Paganoni, AM, Miendlarzewska, EA, Sangalli, LM, Monello, A, Pernow, J, Björnstedt Bennermo, M, Marenzi, G, Hu, D, Uren, NG, Cianflone, D, Ravasi, T, Manfredi, AA & Maseri, A 2012, 'Identification and predictive value of interleukin-6+ interleukin-10+ and interleukin-6-interleukin-10+ cytokine patterns in st-elevation acute myocardial infarction', Circulation Research, vol. 111, no. 10, pp. 1336-1348. https://doi.org/10.1161/CIRCRESAHA.111.262477
Ammirati, Enrico ; Cannistraci, Carlo V. ; Cristell, Nicole A. ; Vecchio, Viviana ; Palini, Alessio G. ; Tornvall, Per ; Paganoni, Anna M. ; Miendlarzewska, Ewa A. ; Sangalli, Laura M. ; Monello, Alberto ; Pernow, John ; Björnstedt Bennermo, Marie ; Marenzi, Giancarlo ; Hu, Dayi ; Uren, Neal G. ; Cianflone, Domenico ; Ravasi, Timothy ; Manfredi, Angelo A. ; Maseri, Attilio. / Identification and predictive value of interleukin-6+ interleukin-10+ and interleukin-6-interleukin-10+ cytokine patterns in st-elevation acute myocardial infarction. In: Circulation Research. 2012 ; Vol. 111, No. 10. pp. 1336-1348.
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T1 - Identification and predictive value of interleukin-6+ interleukin-10+ and interleukin-6-interleukin-10+ cytokine patterns in st-elevation acute myocardial infarction

AU - Ammirati, Enrico

AU - Cannistraci, Carlo V.

AU - Cristell, Nicole A.

AU - Vecchio, Viviana

AU - Palini, Alessio G.

AU - Tornvall, Per

AU - Paganoni, Anna M.

AU - Miendlarzewska, Ewa A.

AU - Sangalli, Laura M.

AU - Monello, Alberto

AU - Pernow, John

AU - Björnstedt Bennermo, Marie

AU - Marenzi, Giancarlo

AU - Hu, Dayi

AU - Uren, Neal G.

AU - Cianflone, Domenico

AU - Ravasi, Timothy

AU - Manfredi, Angelo A.

AU - Maseri, Attilio

PY - 2012/10/26

Y1 - 2012/10/26

N2 - RATIONALE: At the onset of ST-elevation acute myocardial infarction (STEMI), patients can present with very high circulating interleukin-6 (IL-6) levels or very low-IL-6 levels. OBJECTIVE: We compared these 2 groups of patients to understand whether it is possible to define specific STEMI phenotypes associated with outcome based on the cytokine response. METHODS AND RESULTS: We compared 109 patients with STEMI in the top IL-6 level (median, 15.6 pg/mL; IL-6 STEMI) with 96 in the bottom IL-6 level (median, 1.7 pg/mL; IL-6 STEMI) and 103 matched controls extracted from the multiethnic First Acute Myocardial Infarction study. We found minimal clinical differences between IL-6 STEMI and IL-6 STEMI. We assessed the inflammatory profiles of the 2 STEMI groups and the controls by measuring 18 cytokines in blood samples. We exploited clustering analysis algorithms to infer the functional modules of interacting cytokines. IL-6 STEMI patients were characterized by the activation of 2 modules of interacting signals comprising IL-10, IL-8, macrophage inflammatory protein-1α, and C-reactive protein, and monocyte chemoattractant protein-1, macrophage inflammatory protein-1β, and monokine induced by interferon-γ. IL-10 was increased both in IL-6 STEMI and IL-6 STEMI patients compared with controls. IL-6IL-10 STEMI patients had an increased risk of systolic dysfunction at discharge and an increased risk of death at 6 months in comparison with IL-6IL-10 STEMI patients. We combined IL-10 and monokine induced by interferon-γ (derived from the 2 identified cytokine modules) with IL-6 in a formula yielding a risk index that outperformed any single cytokine in the prediction of systolic dysfunction and death. CONCLUSIONS: We have identified a characteristic circulating inflammatory cytokine pattern in STEMI patients, which is not related to the extent of myocardial damage. The simultaneous elevation of IL-6 and IL-10 levels distinguishes STEMI patients with worse clinical outcomes from other STEMI patients. These observations could have potential implications for risk-oriented patient stratification and immune-modulating therapies.

AB - RATIONALE: At the onset of ST-elevation acute myocardial infarction (STEMI), patients can present with very high circulating interleukin-6 (IL-6) levels or very low-IL-6 levels. OBJECTIVE: We compared these 2 groups of patients to understand whether it is possible to define specific STEMI phenotypes associated with outcome based on the cytokine response. METHODS AND RESULTS: We compared 109 patients with STEMI in the top IL-6 level (median, 15.6 pg/mL; IL-6 STEMI) with 96 in the bottom IL-6 level (median, 1.7 pg/mL; IL-6 STEMI) and 103 matched controls extracted from the multiethnic First Acute Myocardial Infarction study. We found minimal clinical differences between IL-6 STEMI and IL-6 STEMI. We assessed the inflammatory profiles of the 2 STEMI groups and the controls by measuring 18 cytokines in blood samples. We exploited clustering analysis algorithms to infer the functional modules of interacting cytokines. IL-6 STEMI patients were characterized by the activation of 2 modules of interacting signals comprising IL-10, IL-8, macrophage inflammatory protein-1α, and C-reactive protein, and monocyte chemoattractant protein-1, macrophage inflammatory protein-1β, and monokine induced by interferon-γ. IL-10 was increased both in IL-6 STEMI and IL-6 STEMI patients compared with controls. IL-6IL-10 STEMI patients had an increased risk of systolic dysfunction at discharge and an increased risk of death at 6 months in comparison with IL-6IL-10 STEMI patients. We combined IL-10 and monokine induced by interferon-γ (derived from the 2 identified cytokine modules) with IL-6 in a formula yielding a risk index that outperformed any single cytokine in the prediction of systolic dysfunction and death. CONCLUSIONS: We have identified a characteristic circulating inflammatory cytokine pattern in STEMI patients, which is not related to the extent of myocardial damage. The simultaneous elevation of IL-6 and IL-10 levels distinguishes STEMI patients with worse clinical outcomes from other STEMI patients. These observations could have potential implications for risk-oriented patient stratification and immune-modulating therapies.

KW - acute myocardial infarction

KW - bioengineering

KW - computational and systems biology

KW - cytokines

KW - inflammation

KW - interleukin-10

KW - interleukin-6

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