Identification and purification of overlapping cosmid clones of the region Xq24-qter of human X chromosome using HPLC

Biunno Ida, Mauri Pierluigi, Susani Lucia, Pietta Piergiorgio

Research output: Contribution to journalArticlepeer-review

Abstract

The assembly of a large physical map of genomes requires simultaneous analysis of many cosmid clones for overlapping regions. The search for overlapping regions may be achieved by various means. High-performance liquid chromatography (HPLC) provides an alternative to gel electrophoresis since microgram amounts of each DNA fragment may be collected into individual test tubes for further analysis. HPLC has been used to identify overlapping cosmid clones from a pool of cosmid DNA containing the terminal portion of the long arm of the human X chromosome (Xq24-qter). Among 400 cosmids analyzed, 3 were shown to overlap.

Original languageEnglish
Pages (from-to)1353-1355
Number of pages3
JournalGenomics
Volume13
Issue number4
DOIs
Publication statusPublished - 1992

ASJC Scopus subject areas

  • Genetics

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