@article{edcc7db8d57c4beaa0a0c6309aed2675,
title = "Identification of a circulating amino acid signature in frail older persons with type 2 diabetes mellitus: Results from the metabofrail study",
abstract = "Diabetes and frailty are highly prevalent conditions that impact the health status of older adults. Perturbations in protein/amino acid metabolism are associated with both functional impairment and type 2 diabetes mellitus (T2DM). In the present study, we compared the concentrations of a panel of circulating 37 amino acids and derivatives between frail/pre-frail older adults with T2DM and robust non-diabetic controls. Sixty-six functionally impaired older persons aged 70+ with T2DM and 30 age and sex-matched controls were included in the analysis. We applied a partial least squares-discriminant analysis (PLS-DA)-based analytical strategy to characterize the metabotype of study participants. The optimal complexity of the PLS-DA model was found to be two latent variables. The proportion of correct classification was 94.1 ± 1.9% for frail/pre-frail persons with T2DM and 100% for control participants. Functionally impaired older persons with T2DM showed higher levels of 3-methyl histidine, alanine, arginine, glutamic acid, ethanolamine sarcosine, and tryptophan. Control participants had higher levels of ornithine and taurine. These findings indicate that a specific profile of amino acids and derivatives characterizes pre-frail/frail older persons with T2DM. The dissection of these pathways may provide novel insights into the metabolic perturbations involved in the disabling cascade in older persons with T2DM.",
keywords = "Aging, Frailty, Metabolic profiling, Metabolism, Metabolomics, Muscle wasting, Personalized medicine, Precision medicine, Sarcopenia, Systems biology",
author = "{European MID-Frail Consortium} and Riccardo Calvani and Leocadio Rodriguez-Ma{\~n}as and Anna Picca and Federico Marini and Alessandra Biancolillo and Olga Laosa and Laura Pedraza and Jacopo Gervasoni and Aniello Primiano and Giorgia Conta and Isabelle Bourdel-Marchasson and Regueme, {Sophie C.} and Roberto Bernabei and Emanuele Marzetti and Sinclair, {Alan J.} and Giovanni Gambassi",
note = "Funding Information: Funding: This work was supported by grants from the European Commission - EU 7th Framework Programme (Contract N◦ 278803), and Innovative Medicine Initiative-Joint Undertaking (IMI-JU #115621). The work was also partly supported by the nonprofit research foundation “Centro Studi Achille e Linda Lorenzon”. The funders had no role in study design, data collection and analysis, preparation of the manuscript, or decision to publish. Funding Information: Conflicts of Interest: A.J.S., E.M., L.R.-M., R.B., and R.C are partners of the SPRINTT Consortium, which is partly funded by the European Federation of Pharmaceutical Industries and Associations (EFPIA). R.C. served as a consultant for Abbott, Novartis, and Nutricia. E.M. served as a consultant for Abbott, Biophytis, Nestl{\`e}, Novartis, and Nutricia. Funding Information: This work was supported by grants from the European Commission-EU 7th Framework Programme (Contract N? 278803), and Innovative Medicine Initiative-Joint Undertaking (IMI-JU # 115621). The work was also partly supported by the nonprofit research foundation ?Centro Studi Achille e Linda Lorenzon?. The funders had no role in study design, data collection and analysis, preparation of the manuscript, or decision to publish. Acknowledgments: The authors thank Luca Mariotti for his precious technical and administrative support. Publisher Copyright: {\textcopyright} 2020 by the authors. Licensee MDPI, Basel, Switzerland. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.",
year = "2020",
month = jan,
doi = "10.3390/nu12010199",
language = "English",
volume = "12",
journal = "Nutrients",
issn = "2072-6643",
publisher = "NLM (Medline)",
number = "1",
}