Identification of a distinct population of CD133 + CXCR4 + cancer stem cells in ovarian cancer

Michele Cioffi, Crescenzo Dalterio, Rosalba Camerlingo, Virginia Tirino, Claudia Consales, Anna Riccio, Caterina Ieranò, Sabrina Chiara Cecere, Nunzia Simona Losito, Stefano Greggi, Sandro Pignata, Giuseppe Pirozzi, Stefania Scala

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

CD133 and CXCR4 were evaluated in the NCI-60 cell lines to identify cancer stem cell rich populations. Screening revealed that, ovarian OVCAR-3, -4 and -5 and colon cancer HT-29, HCT-116 and SW620 over expressed both proteins. We aimed to isolate cells with stem cell features sorting the cells expressing CXCR4 + CD133 + within ovarian cancer cell lines. The sorted population CD133 + CXCR4 + demonstrated the highest efficiency in sphere formation in OVCAR-3, OVCAR-4 and OVCAR-5 cells. Moreover OCT4, SOX2, KLF4 and NANOG were highly expressed in CD133 + CXCR4 + sorted OVCAR-5 cells. Most strikingly CXCR4 + CD133 + sorted OVCAR-5 and -4 cells formed the highest number of tumors when inoculated in nude mice compared to CD133 â ' CXCR4 â ', CD133 + CXCR4 â ', CD133 â ' CXCR4 + cells. CXCR4 + CD133 + OVCAR-5 cells were resistant to cisplatin, overexpressed the ABCG2 surface drug transporter and migrated toward the CXCR4 ligand, CXCL12. Moreover, when human ovarian cancer cells were isolated from 37 primary ovarian cancer, an extremely variable level of CXCR4 and CD133 expression was detected. Thus, in human ovarian cancer cells CXCR4 and CD133 expression identified a discrete population with stem cell properties that regulated tumor development and chemo resistance. This cell population represents a potential therapeutic target.

Original languageEnglish
Article number10357
JournalScientific Reports
Volume5
DOIs
Publication statusPublished - May 28 2015

Fingerprint

Neoplastic Stem Cells
Ovarian Neoplasms
Population
Stem Cells
Cell Line
Nude Mice
Colonic Neoplasms
Cisplatin
Neoplasms
Ligands

ASJC Scopus subject areas

  • General

Cite this

Identification of a distinct population of CD133 + CXCR4 + cancer stem cells in ovarian cancer. / Cioffi, Michele; Dalterio, Crescenzo; Camerlingo, Rosalba; Tirino, Virginia; Consales, Claudia; Riccio, Anna; Ieranò, Caterina; Cecere, Sabrina Chiara; Losito, Nunzia Simona; Greggi, Stefano; Pignata, Sandro; Pirozzi, Giuseppe; Scala, Stefania.

In: Scientific Reports, Vol. 5, 10357, 28.05.2015.

Research output: Contribution to journalArticle

Cioffi M, Dalterio C, Camerlingo R, Tirino V, Consales C, Riccio A et al. Identification of a distinct population of CD133 + CXCR4 + cancer stem cells in ovarian cancer. Scientific Reports. 2015 May 28;5. 10357. https://doi.org/10.1038/srep10357
Cioffi, Michele ; Dalterio, Crescenzo ; Camerlingo, Rosalba ; Tirino, Virginia ; Consales, Claudia ; Riccio, Anna ; Ieranò, Caterina ; Cecere, Sabrina Chiara ; Losito, Nunzia Simona ; Greggi, Stefano ; Pignata, Sandro ; Pirozzi, Giuseppe ; Scala, Stefania. / Identification of a distinct population of CD133 + CXCR4 + cancer stem cells in ovarian cancer. In: Scientific Reports. 2015 ; Vol. 5.
@article{32eb4de0ba0048999482cd778ad3881d,
title = "Identification of a distinct population of CD133 + CXCR4 + cancer stem cells in ovarian cancer",
abstract = "CD133 and CXCR4 were evaluated in the NCI-60 cell lines to identify cancer stem cell rich populations. Screening revealed that, ovarian OVCAR-3, -4 and -5 and colon cancer HT-29, HCT-116 and SW620 over expressed both proteins. We aimed to isolate cells with stem cell features sorting the cells expressing CXCR4 + CD133 + within ovarian cancer cell lines. The sorted population CD133 + CXCR4 + demonstrated the highest efficiency in sphere formation in OVCAR-3, OVCAR-4 and OVCAR-5 cells. Moreover OCT4, SOX2, KLF4 and NANOG were highly expressed in CD133 + CXCR4 + sorted OVCAR-5 cells. Most strikingly CXCR4 + CD133 + sorted OVCAR-5 and -4 cells formed the highest number of tumors when inoculated in nude mice compared to CD133 {\^a} ' CXCR4 {\^a} ', CD133 + CXCR4 {\^a} ', CD133 {\^a} ' CXCR4 + cells. CXCR4 + CD133 + OVCAR-5 cells were resistant to cisplatin, overexpressed the ABCG2 surface drug transporter and migrated toward the CXCR4 ligand, CXCL12. Moreover, when human ovarian cancer cells were isolated from 37 primary ovarian cancer, an extremely variable level of CXCR4 and CD133 expression was detected. Thus, in human ovarian cancer cells CXCR4 and CD133 expression identified a discrete population with stem cell properties that regulated tumor development and chemo resistance. This cell population represents a potential therapeutic target.",
author = "Michele Cioffi and Crescenzo Dalterio and Rosalba Camerlingo and Virginia Tirino and Claudia Consales and Anna Riccio and Caterina Ieran{\`o} and Cecere, {Sabrina Chiara} and Losito, {Nunzia Simona} and Stefano Greggi and Sandro Pignata and Giuseppe Pirozzi and Stefania Scala",
year = "2015",
month = "5",
day = "28",
doi = "10.1038/srep10357",
language = "English",
volume = "5",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",

}

TY - JOUR

T1 - Identification of a distinct population of CD133 + CXCR4 + cancer stem cells in ovarian cancer

AU - Cioffi, Michele

AU - Dalterio, Crescenzo

AU - Camerlingo, Rosalba

AU - Tirino, Virginia

AU - Consales, Claudia

AU - Riccio, Anna

AU - Ieranò, Caterina

AU - Cecere, Sabrina Chiara

AU - Losito, Nunzia Simona

AU - Greggi, Stefano

AU - Pignata, Sandro

AU - Pirozzi, Giuseppe

AU - Scala, Stefania

PY - 2015/5/28

Y1 - 2015/5/28

N2 - CD133 and CXCR4 were evaluated in the NCI-60 cell lines to identify cancer stem cell rich populations. Screening revealed that, ovarian OVCAR-3, -4 and -5 and colon cancer HT-29, HCT-116 and SW620 over expressed both proteins. We aimed to isolate cells with stem cell features sorting the cells expressing CXCR4 + CD133 + within ovarian cancer cell lines. The sorted population CD133 + CXCR4 + demonstrated the highest efficiency in sphere formation in OVCAR-3, OVCAR-4 and OVCAR-5 cells. Moreover OCT4, SOX2, KLF4 and NANOG were highly expressed in CD133 + CXCR4 + sorted OVCAR-5 cells. Most strikingly CXCR4 + CD133 + sorted OVCAR-5 and -4 cells formed the highest number of tumors when inoculated in nude mice compared to CD133 â ' CXCR4 â ', CD133 + CXCR4 â ', CD133 â ' CXCR4 + cells. CXCR4 + CD133 + OVCAR-5 cells were resistant to cisplatin, overexpressed the ABCG2 surface drug transporter and migrated toward the CXCR4 ligand, CXCL12. Moreover, when human ovarian cancer cells were isolated from 37 primary ovarian cancer, an extremely variable level of CXCR4 and CD133 expression was detected. Thus, in human ovarian cancer cells CXCR4 and CD133 expression identified a discrete population with stem cell properties that regulated tumor development and chemo resistance. This cell population represents a potential therapeutic target.

AB - CD133 and CXCR4 were evaluated in the NCI-60 cell lines to identify cancer stem cell rich populations. Screening revealed that, ovarian OVCAR-3, -4 and -5 and colon cancer HT-29, HCT-116 and SW620 over expressed both proteins. We aimed to isolate cells with stem cell features sorting the cells expressing CXCR4 + CD133 + within ovarian cancer cell lines. The sorted population CD133 + CXCR4 + demonstrated the highest efficiency in sphere formation in OVCAR-3, OVCAR-4 and OVCAR-5 cells. Moreover OCT4, SOX2, KLF4 and NANOG were highly expressed in CD133 + CXCR4 + sorted OVCAR-5 cells. Most strikingly CXCR4 + CD133 + sorted OVCAR-5 and -4 cells formed the highest number of tumors when inoculated in nude mice compared to CD133 â ' CXCR4 â ', CD133 + CXCR4 â ', CD133 â ' CXCR4 + cells. CXCR4 + CD133 + OVCAR-5 cells were resistant to cisplatin, overexpressed the ABCG2 surface drug transporter and migrated toward the CXCR4 ligand, CXCL12. Moreover, when human ovarian cancer cells were isolated from 37 primary ovarian cancer, an extremely variable level of CXCR4 and CD133 expression was detected. Thus, in human ovarian cancer cells CXCR4 and CD133 expression identified a discrete population with stem cell properties that regulated tumor development and chemo resistance. This cell population represents a potential therapeutic target.

UR - http://www.scopus.com/inward/record.url?scp=84930683609&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84930683609&partnerID=8YFLogxK

U2 - 10.1038/srep10357

DO - 10.1038/srep10357

M3 - Article

AN - SCOPUS:84930683609

VL - 5

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

M1 - 10357

ER -