Identification of a glucocorticoid response element in the human γ chain fibrinogen promoter

Rosanna Asselta, Stefano Duga, Michele Modugno, Massimo Malcovati, Maria Luisa Tenchini

Research output: Contribution to journalArticle

Abstract

The effect of the synthetic glucocorticoid hormone dexamethasone on human γ chain fibrinogen gene expression was examined. The whole promoter region of 3.8 kb of this gene and progressive 5'-deletions were inserted into a promoterless expression vector, upstream of the luciferase gene and transiently transfected into the human hepatoma HepG2 cells, in the presence or in the absence of dexamethasone stimulation. Deletion analysis allowed to identify a region located between -1359 and -954 bp upstream from the transcription start site, involved in hormone inducibility. On the basis of a computer-assisted analysis, a putative GRE was found in this region at bases -1116 to -1102. Specific point mutations eliminating this putative GRE led to complete loss of glucocorticoid inducibility, thus indicating its functional role. Binding of the rat glucocorticoid receptor to this site was demonstrated by mobility-shift assays.

Original languageEnglish
Pages (from-to)1144-1150
Number of pages7
JournalThrombosis and Haemostasis
Volume79
Issue number6
Publication statusPublished - 1998

ASJC Scopus subject areas

  • Hematology

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