Identification of a human cytomegalovirus mutant in the pp150 matrix phosphoprotein gene with a growth-defective phenotype

D. Zipeto, F. Baldanti, E. Percivalle, G. Gerna, G. Milanesi

Research output: Contribution to journalArticlepeer-review

Abstract

Following amplification by PCR of a portion of the matrix phosphoprotein pp150 gene, electrophoretic analysis revealed the simultaneous presence of two viral variants of human cytomegalovirus in the blood of a heart transplant recipient. Repeated denaturation-annealing cycles during the amplification reaction led to the formation of heteroduplex molecules with altered electrophoretic mobility. Sequence analysis of the amplification products showed the presence of a viral variant carrying an in-frame three nucleotide deletion, which caused the absence of an aspartic acid in the corresponding protein. Attempts to plaque-purify the deletion mutant were unsuccessful, suggesting that the variant was growth-defective.

Original languageEnglish
Pages (from-to)1645-1648
Number of pages4
JournalJournal of General Virology
Volume74
Issue number8
Publication statusPublished - 1993

ASJC Scopus subject areas

  • Immunology
  • Virology

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